Evaluation of Single-nucleotide primer extension for detection and typing of phylogenetic markers used for investigation of microbial communities

Applied and Environmental Microbiology

Volumn 75, Issue 9, 2009, Pages 2850-2860

  Nikolausz, Marcell ^a   Chatzinotas, Antonis ^a   Táncsics, András ^b   Imfeld, Gwenaël ^a   Kästner, Matthias ^a  

^a HELMHOLTZ CENTRE FOR ENVIRONMENTAL RESEARCH UFZ   (Germany)

^b EÖTVÖS LORÁND UNIVERSITY   (Hungary)

Author keywords

[No Author keywords available]

Indexed keywords

16S RRNA; 16S RRNA GENE; BACTERIAL CULTURES; DETECTION SENSITIVITY; FLUORESCENT LABELING; HIGH SIGNAL-TO-NOISE RATIO; HYBRID DUPLEX; MICROBIAL COMMUNITIES; PARALLEL DETECTION; PCR PRODUCTS; PRIMER EXTENSION; SIGNAL RATIOS; SIMULTANEOUS DETECTION; SPECIFIC PRIMERS;

DNA SEQUENCES; FADING (RADIO); MULTIPLEXING; NUCLEIC ACIDS; NUCLEOTIDES; SIGNAL TO NOISE RATIO;

SIGNAL DETECTION;

DNA; FLUORESCENT DYE; GENE PRODUCT; RNA 16S; PRIMER DNA; RIBOSOME DNA;

BACTERIUM; BIOENGINEERING; DETECTION METHOD; DNA; GENE EXPRESSION; GENETIC MARKER; HYBRIDIZATION; MICROBIAL COMMUNITY; PHYLOGENETICS; POLYMERASE CHAIN REACTION; RNA;

ARTICLE; BACTERIUM CULTURE; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; DNA DETERMINATION; DNA HYBRIDIZATION; DNA TEMPLATE; EXTENSION GENE; MICROBIAL COMMUNITY; MOLECULAR PHYLOGENY; NONHUMAN; NUCLEIC ACID ANALYSIS; NUCLEOTIDE SEQUENCE; POLYMERASE CHAIN REACTION; RNA ANALYSIS; SIGNAL NOISE RATIO; SINGLE NUCLEOTIDE PRIMER EXTENSION; BACTERIUM; CLASSIFICATION; EVALUATION; GENETICS; METHODOLOGY; MOLECULAR BIOLOGY; MOLECULAR GENETICS; SENSITIVITY AND SPECIFICITY;

BACTERIA (MICROORGANISMS);

BACTERIA; BASE SEQUENCE; DNA PRIMERS; DNA, RIBOSOMAL; MOLECULAR BIOLOGY; MOLECULAR SEQUENCE DATA; RNA, RIBOSOMAL, 16S; SENSITIVITY AND SPECIFICITY;

EID: 65549169440     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.01910-08     Document Type: Article

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