Immunogenicity of a Cholera Toxin B Subunit Porphyromonas gingivalis Fimbrial Antigen Fusion Protein Expressed in E. coli

Molecular Biotechnology

Volumn 41, Issue 2, 2009, Pages 157-164

  Kim, Tae Geum ^a   Huy, Nguyen Xuan ^a   Kim, Mi Young ^a   Jeong, Dong Keun ^a   Jang, Yong Suk ^a   Yang, Moon Sik ^a   Langridge, William H R ^b   Lee, Jin Yong ^c  

^a Chonbuk National University   (South Korea)

^b LOMA LINDA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^c Kyung Hee University   (South Korea)

Author keywords

Cholera toxin B subunit; Fimbrial antigen; Periodontal disease; Porphyromonas gingivalis

Indexed keywords

AMINO ACID RESIDUES; ANTIBODY TITERS; B CELLS; BINDING ACTIVITIES; C-TERMINUS; CHOLERA TOXIN B SUBUNIT; CLASS-SWITCHING; DENATURING CONDITIONS; E. COLI; EXPERIMENTAL DATA; EXPRESSION VECTORS; FIMBRIAL ANTIGEN; FRAGMENT ENCODING; FUSION PROTEINS; IMMUNOBLOT ANALYSIS; IMMUNOGENICITY; IMMUNOMODULATION; ORAL BACTERIA; PENTAMERS; PERIODONTAL DISEASE; PORPHYROMONAS GINGIVALIS; PROTEIN SEQUENCES; RENATURATION; SUBUNIT VACCINE;

AMINATION; AMINES; AMINO ACIDS; ANTIGENS; BODY FLUIDS; CELL MEMBRANES; CHROMATOGRAPHIC ANALYSIS; COLUMN CHROMATOGRAPHY; ENCODING (SYMBOLS); ESCHERICHIA COLI; ORGANIC ACIDS; PURIFICATION; TOXIC MATERIALS;

ANTIBODIES;

BACTERIAL VECTOR; CHOLERA TOXIN B SUBUNIT; CHOLERA TOXIN B SUBUNIT FIMBRIA A1 FUSION PROTEIN; CHOLERA TOXIN B SUBUNIT FIMBRIA A2 FUSION PROTEIN; COMPLEMENTARY DNA; FIMBRIA A1 PROTEIN; FIMBRIA A2 PROTEIN; GANGLIOSIDE GM1; HYBRID PROTEIN; IMMUNOGLOBULIN A ANTIBODY; IMMUNOGLOBULIN G ANTIBODY; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ADJUVANT THERAPY; AMINO ACID SEQUENCE; ANIMAL EXPERIMENT; ANTIBODY RESPONSE; ANTIBODY TITER; ARTICLE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; DOWNSTREAM PROCESSING; DRUG EFFICACY; DRUG POTENTIATION; DRUG PURIFICATION; DRUG RECEPTOR BINDING; DRUG SYNTHESIS; ESCHERICHIA COLI; EXPRESSION VECTOR; FEASIBILITY STUDY; FIMBRIA; IMMUNOGENICITY; IMMUNOMODULATION; IMMUNOSTIMULATION; MOLECULAR CLONING; MOUSE; NONHUMAN; PORPHYROMONAS GINGIVALIS; PROTEIN DENATURATION; VACCINATION;

ADMINISTRATION, ORAL; ANTIBODIES, BACTERIAL; BACTERIAL VACCINES; BACTEROIDACEAE INFECTIONS; CHOLERA TOXIN; ESCHERICHIA COLI; FECES; FIMBRIAE PROTEINS; IMMUNIZATION; IMMUNOGLOBULIN A; IMMUNOGLOBULIN G; PORPHYROMONAS GINGIVALIS; RECOMBINANT FUSION PROTEINS;

BACTERIA (MICROORGANISMS); MUS; NEGIBACTERIA; PORPHYROMONAS GINGIVALIS;

EID: 65549169379     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12033-008-9102-3     Document Type: Article

References (32)

1. RJ Lamont HF Jenkinson 1998 Life below the gun line: Pathogenic mechanisms of Porphyromonas gingivalis Microbiology and Molecular Biology Reviews 62 1244 1263
2. S Hamada A Amano S Kimura I Nakagawa S Kawabata I Morisaki 1998 The importance of fimbriae in the virulence and ecology of some oral bacteria Oral Microbiology and Immunology 13 129 138 10.1111/j.1399-302X.1998.tb00724.x
3. J-Y Lee HT Sojar GS Bedi RJ Genco 1992 Synthetic peptides analogous to the fimbrillin sequence inhibit adherence of Porphyromonas gingivalis Infection and Immunity 60 1662 1670
4. A Amano A Sharma J-Y Lee HT Sojar PA Raj RJ Genco 1996 Structural domains of Porphyromonas gingivalis recombinant fimbrillin that mediate binding to salivary praline-rich protein and statherin Infection and Immunity 64 1631 1637
5. HT Sojar J-Y Lee RJ Genco 1995 Fibronectin binding domain of P. gingivalis fimbriae Biochemical and Biophysical Research Communications 216 785 792 10.1006/bbrc.1995.2690
6. HT Sojar A Sharma RJ Genco 2002 Porphyromonas gingivalis fimbriae bind to cytokeratin of epithelial cells Infection and Immunity 70 96 101 10.1128/IAI.70.1.96-101.2002 (Pubitemid 34028603)
7. R Jotwani CW Cutler 2004 Fimbriated Porphyromonas gingivalis is more efficient than fimbriae-deficient P. gingivalis in entering human dendritic cells in vitro and induces inflammatory Th1 effector response Infection and Immunity 72 1725 1732 10.1128/IAI.72.3.1725-1732.2004 (Pubitemid 38263797)
8. G Hajishengalis H Sojar RJ Genco E DeNardin 2004 Intracellular signaling and cytokine induction upon interactions of Porphyromonas gingivalis fimbriae with pattern-recognition receptors Immunological Investigations 33 157 172 10.1081/IMM-120030917
9. AM DeNardin HT Sojar SG Grossi LA Christersson RJ Genco 1991 Humoral immunity of older adults with periodontal disease to Porphyromonas gingivalis Infection and Immunity 59 4363 4370
10. F Condorelli G Scalia G Cali B Rossetti G Nicoletti AM Lo Bue 1998 Isolation of Porphyromonas gingivalis and detection of immunoglobulin A specific to fimbrial antigen in gingival crevicular fluid Journal of Clinical Microbiology 36 2322 2325
11. H Isogai F Yoshimura T Suzuki W Kagota K Takano 1988 Specific inhibition of adherence of an oral strain of Bacteroides gingivalis 381 to epithelial cells by monoclonal antibodies against the bacterial fimbriae Archives of Oral Biology 33 479 485 10.1016/0003-9969(88)90028-3
12. Q Fan T Sims H Sojar R Genco RC Page 2001 Fimbriae of Porphyromonas gingivalis induce opsonic antibodies that significantly enhance phagocytosis and killing by human polymorphonuclear leukocytes Oral Microbiology and Immunology 16 144 152 10.1034/j.1399-302X.2001.016003144.x (Pubitemid 32418003)
13. R Malek JG Fisher A Caleca M Stinson CJ Van Oss J-Y Lee 1994 Inactivation of the Porphyromonas gingivalis fimA gene blocks periodontal damage in gnotobiotic rats Journal of Bacteriology 176 1052 1059
14. A Weinberg CA Belton Y Park RJ Lamont 1997 Role of fimbriae in Porphyromonas gingivalis invasion of gingival epithelial cells Infection and Immunity 65 313 316
15. I Umemoto N Hamada 2003 Characterization of biologically active cell surface components of a periodontal pathogen. The roles of major and minor fimbriae of Porphyromonas gingivalis Journal of Periodontology 74 119 122 10.1902/jop.2003.74.1.119 (Pubitemid 36175743)
16. T Arakawa J Yu WHR Langridge 2001 Synthesis of a cholera toxin B subunit-rotavirus NSP4 fusion protein in potato Plant Cell Reports 20 343 348 10.1007/s002990000312 (Pubitemid 32623620)
17. TG Kim N Befus WHR Langridge 2004 Co-immunization with an HIV-1 Tat transduction peptide-rotavirus enterotoxin fusion protein stimulates Th1 mucosal immune response in mice Vaccine 22 431 438 10.1016/j.vaccine.2003.07.015 (Pubitemid 37510274)
18. MT Dertzbaugh CO Elson 1993 Comparative effectiveness of the cholera toxin B subunits and alkaline phosphatase as carrier for oral vaccines Infection and Immunity 61 48 55
19. JB Sun J Holmgren C Czerkinsky 1994 Cholera toxin B subunit: An efficient transmucosal carrier-delivery system for induction of peripheral immunological tolerance Proceedings of the National Academy of Sciences of the United States of America 93 7196 7201 10.1073/pnas.93.14.7196
20. HL Weiner 1994 Oral tolerance Proceedings of the National Academy of Sciences of the United States of America 91 10762 10765 10.1073/pnas.91.23.10762 (Pubitemid 24349564)
21. G Hajishengallis P Ratti E Harokopakis 2005 Peptide mapping of bacterial fimbrial epitopes interacting with pattern recognition receptors The Journal of Biological Chemistry 280 38902 38913 10.1074/jbc.M507326200 (Pubitemid 41713839)
22. T Ogawa Y Kusumoto H Uchida S Nagashima H Ogo S Hamada 1991 Immunological activities of synthetic peptide segments of fimbrial protein from Porphyromonas gingivalis Biochemical and Biophysical Research Communications 180 1335 1341 10.1016/S0006-291X(05)81342-7
23. T Ogawa 1994 The potential protective immune responses to synthetic peptides containing conserved epitopes of Porphyromonas gingivalis fimbrial protein Journal of Medical Microbiology 41 349 358
24. H Nagata A Sharma HT Sojar A Amano MJ Levine RJ Genco 1997 Role of the carboxyl-terminal regions of Porphyromonas gingivalis fimbrillin in binding to salivary proteins Infection and Immunity 65 422 427
25. TG Kim WHR Langridge 2003 Assembly of cholera toxin B subunit full-length rotavirus NSP4 fusion protein oligomers in transgenic potato Plant Cell Reports 21 884 890
26. C Jespersgaard G Hajishengallis TE Greenway DJ Smith MW Russell SM Michalek 1999 Functional and immunogenic characterization of two cloned regions of Streptococcus mutans glucosyltransferase I Infection and Immunity 67 810 816
27. J-Y Lee HT Sojar A Amano RJ Genco 1995 Purification of major fimbrial proteins of Porphyromonas gingivalis Protein Expression and Purification 6 496 500 10.1006/prep.1995.1066
28. WJ Loesche NS Grossman 2001 Periodontal disease as a specific, albeit chronic, infection: Diagnosis and treatment Clinical Microbiology Reviews 14 727 752 10.1128/CMR.14.4.727-752.2001 (Pubitemid 32979629)
29. MT Dertzbaugh CO Elson 1993 Reduction in oral immunogenicity of the cholera toxin B subunits by N-terminal peptide addition Infection and Immunity 61 384 390
30. S Liljeqvist S Stahl C Andreoni H Binz M Uhlen M Murby 1997 Fusions to the cholera toxin B subunit: influence on pentamerization and GM1 binding Journal of Immunological Methods 210 125 135 10.1016/S0022-1759(97)00170-1 (Pubitemid 28266557)
31. Mitchell, V. S., Philipose, N. M., & Sanford, J. P. (1993). The children's vaccine initiative. National Academy Press.
32. E-A Shin J-Y Lee T-G Kim YK Park WHR Langridge 2006 Synthesis and assembly of an adjuvanted Porphyromonas gingivalis fimbrial antigen fusion protein in plants Protein Expression and Purification 47 99 109 10.1016/j.pep.2005.09.005 (Pubitemid 44389377)