Role of the carboxyl-terminal region of Porphyromonas gingivalis fimbrillin in binding to salivary proteins

Infection and Immunity

Volumn 65, Issue 2, 1997, Pages 422-427

  Nagata, Hideki ^a   Sharma, Ashu ^a   Sojar, Hakimuddin T ^a   Amano, Atsuo ^b   Levine, Michael J ^a   Genco, Robert J ^a,c  

^a UNIVERSITY AT BUFFALO   (United States)

^b OSAKA UNIVERSITY   (Japan)

^c UNIVERSITY AT BUFFALO   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

HYDROXYAPATITE; STATHERIN;

ARTICLE; BACTERIAL COLONIZATION; BACTERIUM ADHERENCE; BINDING SITE; CARBOXY TERMINAL SEQUENCE; FIMBRIA; MOUTH CAVITY; NONHUMAN; PORPHYROMONAS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN PURIFICATION;

EID: 0031017469     PISSN: 00199567     EISSN: None     Source Type: Journal    
DOI: 10.1128/iai.65.2.422-427.1997     Document Type: Article

References (26)

1. Amano, A., A. Sharma, J.-Y. Lee, H. T. Sojar, P. A. Raj, and R. J. Genco. 1996. Structural binding domains of Porphyromonas gingivalis recombinant fimbrillin to salivary prolinc-rich protein and statherin. Infect. Immun. 64: 1631-1637.
2. Amano, A., H. T. Sojar, J.-Y. Lee, A. Sharma, M. J. Levine, and R. J. Genco. 1994. Salivary receptors for recombinant fimbrillin of Porphyromonas gingivalis. Infect. Immun. 62:3372-3380.
3. Birnboim, H. C., and J. Doly. 1979. A rapid alkaline lysis extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 7:1513-1523.
4. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-254.
5. Bradway, S. D., E. J. Bergey, F. A. Scannapieco, N. Ramasubbu, S. Zawack, and M. J. Levine. 1992. Formation of salivary-mucosal pellicle: the role of transglutaminase. Biochem. J. 284:557-564.
6. Dickinson, D. P., M. K. Kubiniec, F. Yoshimura, and R. J. Genco. 1988. Molecular cloning and sequencing of the gene encoding the fimbrial subunit protein of Bacteroides gingivalis. J. Bacteriol. 170:1658-1665.
7. Fujiwara, T., S. Morishima, I. Takahashi, and S. Hamada. 1993. Molecular cloning and sequence of the fimbrillin gene of Porphyromonas gingivalis strains and characterization of recombinant proteins. Biochem. Biophys. Res. Commun. 197:241-247.
8. Goulbourne, P. A., and R. P. Ellen. 1991. Evidence that Porphyromonas (Bacteroides)gingivalis fimbriae function in adhesion to Actinomyces viscosus. J. Bacteriol. 173:5266-5274.
9. Hunter, W. M., and F. C. Greenwood. 1962. Preparation of iodine-131 labeled human growth hormone of high specific activity. Nature (London) 194:495-496.
10. Isogai, H., E. Isogai, F. Yoshimura, T. Suzuki, W. Kagota, and K. Takano. 1988. Specific inhibition of adherence of an oral strain of Bacteroides gingivalis 381 to epithelial cells by monoclonal antibodies against the bacterial fimbriae. Arch. Oral Biol. 33:479-485.
11. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227:680-685.
12. Lamont, R. J., C. A. Bavan, S. Gil, R. E. Persson, and B. Rosan. 1993. Involvement of Porphyromonas gingivalis fimbriae in adherence to Streptococcus gordonii. Oral Microbiol. Immunol. 8:272-276.
13. Lee, J.-Y., H. T. Sojar, G. S. Bedi, and R. J. Genco. 1992. Synthetic peptides analogous to the fimbrillin sequence inhibit adherence of Porphyromonas gingivalis. Infect. Immun. 60:1662-1670.
14. Lee, J.-Y., H. T. Sojar, G. S. Bedi, and R. J. Genco. 1991. Porphyromonas gingivalis fimbrillin: size, amino-terminal sequence, and antigenic heterogeneity. Infect. Immun. 59:383-389.
15. Malek, R., J. G. Fisher, A. Caleca, M. Stinson, C. J. vanOss, J.-Y. Lee, M.-I. Cho, R. J. Genco, R. T. Evans, and D. W. Dyer. 1994. Inactivation of the Porphyromonas gingivalis fimA gene blocks periodontal damage in gnotobiotic rats. J. Bacteriol. 176:1052-1059.
16. Mayrand, D., and S. C. Holt. 1988. Biology of asaccharolytic black-pigmented Bacteroides species. Microbiol. Rev. 52:134-152.
17. Naito, Y., H. Tohda, K. Okuda, and I. Takazoe. 1993. Adherence and hydrophobicity of invasive and noninvasive strains of Porphyromonas gingivalis. Oral Microbiol. Immunol. 8:195-202.
18. Okuda, K. 1993. Attachment mechanisms and colonization, p. 139-158. In H. N. Shah, D. Mayrand, and R. J. Genco (ed.), Biology of the species Porphyromonas gingivalis. CRC Press Inc., Boca Raton, Fla.
19. Ramasubbu, N., M. S. Reddy, E. J. Bergey, G. G. Haraszthy, S.-D. Soni, and M. J. Levine. 1991. Large-scale purification and characterization of the major phosphoproteins and mucins of human submandibular-sublingual saliva. Biochem. J. 280:341-352.
20. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
21. Sharma, A., H. T. Sojar, J.-Y. Lee, and R. J. Genco. 1993. Expression of a functional Porphyromonas gingivalis fimbrillin polypeptide in Escherichia coli: purification, physicochemical and immunochemical characterization, and binding characteristics. Infect. Immun. 61:3570-3573.
22. Shomers, J. P., L. A. Tabak, M. J. Levine, I. D. Mandel, and S. A. Ellison. 1982. The isolation of a family of cysteine-containing phosphoproteins from human submandibular-sublingual saliva. J. Dent. Res. 61:973-977.
23. Slots, J., and R. J. Gibbons. 1978. Attachment of Bacteroides melaninogenicus subsp. asaccharolyticus to oral surfaces and its possible role in colonization of the mouth and of periodontal pockets. Infect. Immun. 19:256-264.
24. Sojar, H. T., J.-Y. Lee, and R. J. Genco. 1995. Fibronectin binding domain of P. gingivalis fimbriae. Biochem. Biophys. Res. Commun. 216:785-792.
25. Sojar, H. T., J.-Y. Lee, G. S. Bedi, M.-I. Cho, and R. J. Genco. 1991. Purification, characterization and immunolocalization of fimbrial protein from Porphyromonas (Bacteroides) gingivalis. Biochem. Biophys. Res. Commun. 175:713-719.
26. Zambon, J. J. 1990. Microbiology of periodontal disease, p. 147-160. In R. J. Genco, H. M. Goldman, and D. W. Cohen (ed.), Contemporary periodontics. The C. V. Mosby Co., St. Louis, Mo.