Fluorescence fluctuation spectroscopy on viral-like particles reveals variable Gag stoichiometry

Biophysical Journal

Volumn 96, Issue 5, 2009, Pages 1961-1969

  Chen, Yan ^a,d   Wu, Bin ^a   Musier Forsyth, Karin ^b   Mansky, Louis M ^c,d   Mueller, Joachim D ^a,d  

^a University of Minnesota   (United States)

^b OHIO STATE UNIVERSITY   (United States)

^c UNIVERSITY OF MINNESOTA   (United States)

^d UNIVERSITY OF MINNESOTA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GAG PROTEIN; GREEN FLUORESCENT PROTEIN; PLASMID DNA;

ANIMAL CELL; ARTICLE; BRIGHTNESS; CELL STRAIN COS1; CHEMICAL LABELING; COMPLEX FORMATION; CONTROLLED STUDY; FLUORESCENCE SPECTROSCOPY; GENETIC TRANSFECTION; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PARTICLE SIZE; PROTEIN ANALYSIS; PROTEIN EXPRESSION; STOICHIOMETRY; VIRUS ENVELOPE; VIRUS LIKE AGENT; ANIMAL; CERCOPITHECUS; CHEMISTRY; METHODOLOGY; MICROPHOTOGRAPHY; POLYACRYLAMIDE GEL ELECTROPHORESIS; SPECTROFLUOROMETRY; STATISTICAL MODEL; TRANSMISSION ELECTRON MICROSCOPY;

HUMAN IMMUNODEFICIENCY VIRUS 1;

ANIMALS; CERCOPITHECUS AETHIOPS; COS CELLS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; GAG GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; HIV-1; LINEAR MODELS; MICROSCOPY, ELECTRON, TRANSMISSION; PHOTOMICROGRAPHY; SPECTROMETRY, FLUORESCENCE; TRANSFECTION;

EID: 65549100184     PISSN: 00063495     EISSN: 15420086     Source Type: Journal    
DOI: 10.1016/j.bpj.2008.10.067     Document Type: Article

References (36)

1. Thompson, N. L., A. M. Lieto, and N. W. Allen. 2002. Recent advances in fluorescence correlation spectroscopy. Curr. Opin. Struct. Biol. 12:634-641.
2. Magde, D., E. Elson, and W. W. Webb. 1972. Thermodynamic fluctuations in a reacting system: measurement by fluorescence correlation spectroscopy. Phys. Rev. Lett. 29:705-708.
3. Chen, Y., J. D. Müller, P. T. C. So, and E. Gratton. 1999. The photon counting histogram in fluorescence fluctuation spectroscopy. Biophys. J. 77:553-567.
4. Berland, K. M. 2004. Fluorescence correlation spectroscopy: a new tool for quantification of molecular interactions. Methods Mol. Biol. 261:383-398.
5. Hwang, L. C., and T. Wohland. 2007. Recent advances in fluorescence cross-correlation spectroscopy. Cell Biochem. Biophys. 49:1-13.
6. Kohl, T., and P. Schwille. 2005. Fluorescence correlation spectroscopy with autofluorescent proteins. Adv. Biochem. Eng. Biotechnol. 95: 107-142.
7. Kolin, D. L., and P. W. Wiseman. 2007. Advances in image correlation spectroscopy: measuring number densities, aggregation states, and dynamics of fluorescently labeled macromolecules in cells. Cell Biochem. Biophys. 49:141-164.
8. Saffarian, S., Y. Li, E. L. Elson, and L. J. Pike. 2007. Oligomerization of the EGF receptor investigated by live cell fluorescence intensity distribution analysis. Biophys. J. 93:1021-1031.
9. Thompson, N. L., and B. L. Steele. 2007. Total internal reflection with fluorescence correlation spectroscopy. Nat. Protocols. 2:878-890.
10. Chen, Y., L. N. Wei, and J. D. Müller. 2003. Probing protein oligomerization in living cells with fluorescence fluctuation spectroscopy. Proc. Natl. Acad. Sci. USA. 100:15492-15497.
11. Chen, Y., and J. D. Müller. 2007. Determining the stoichiometry of protein hetero-complexes in living cells with fluorescence fluctuation spectroscopy. Proc. Natl. Acad. Sci. USA. 104:3147-3152.
12. Chen, Y., L. N. Wei, and J. D. Müller. 2005. Unraveling protein-protein interactions in living cells with fluorescence fluctuation brightness analysis. Biophys. J. 88:4366-4377.
13. Gheysen, D., E. Jacobs, F. de Foresta, C. Thiriart, M. Francotte, et al. 1989. Assembly and release of HIV-1 precursor Pr55gag virus-like particles from recombinant baculovirus-infected insect cells. Cell. 59:103-112.
14. Karacostas, V., K. Nagashima, M. A. Gonda, and B. Moss. 1989. Human immunodeficiency virus-like particles produced by a vaccinia virus expression vector. Proc. Natl. Acad. Sci. USA. 86:8964-8967.
15. Briggs, J. A., M. N. Simon, I. Gross, H. G. Krausslich, S. D. Fuller, et al. 2004. The stoichiometry of Gag protein in HIV-1. Nat. Struct. Mol. Biol. 11:672-675.
16. Layne, S. P., M. J. Merges, M. Dembo, J. L. Spouge, S. R. Conley, et al. 1992. Factors underlying spontaneous inactivation and susceptibility to neutralization of human immunodeficiency virus. Virology. 189: 695-714.
17. Piatak, M., Jr., M. S. Saag, L. C. Yang, S. J. Clark, J. C. Kappes, et al. 1993. High levels of HIV-1 in plasma during all stages of infection determined by competitive PCR. Science. 259:1749-1754.
18. Vogt, V. M., and M. N. Simon. 1999. Mass determination of Rous sarcoma virus virions by scanning transmission electron microscopy. J. Virol. 73:7050-7055.
19. Wright, E. R., J. B. Schooler, H. J. Ding, C. Kieffer, C. Fillmore, et al. 2007. Electron cryotomography of immature HIV-1 virions reveals the structure of the CA and SP1 Gag shells. EMBO J. 26:2218-2226.
20. Yeager, M., E. M. Wilson-Kubalek, S. G. Weiner, P. O. Brown, and A. Rein. 1998. Supramolecular organization of immature and mature murine leukemia virus revealed by electron cryo-microscopy: implications for retroviral assembly mechanisms. Proc. Natl. Acad. Sci. USA. 95:7299-7304.
21. Zhu, P., E. Chertova, J. Bess, Jr., J. D. Lifson, L. O. Arthur, et al. 2003. Electron tomography analysis of envelope glycoprotein trimers on HIV and simian immunodeficiency virus virions. Proc. Natl. Acad. Sci. USA. 100:15812-15817.
22. Derdowski, A., L. Ding, and P. Spearman. 2004. A novel fluorescence resonance energy transfer assay demonstrates that the human immunodeficiency virus type 1 Pr55Gag I domain mediates Gag-Gag interactions. J. Virol. 78:1230-1242.
23. Hillesheim, L. N., and J. D. Müller. 2003. The photon counting histogram in fluorescence fluctuation spectroscopy with non-ideal photodetectors. Biophys. J. 85:1948-1958.
24. Müller, J. D., Y. Chen, and E. Gratton. 2000. Resolving heterogeneity on the single molecular level with the photon counting histogram. Biophys. J. 78:474-486.
25. Doultree, J. C., R. E. Kiernan, J. Y. Lee, D. S. Bowden, D. A. McPhee, et al. 1992. A new electron microscope positive staining method for viruses in suspension. J. Virol. Methods. 37:321-335.
26. Wu, B., Y. Chen, and J. D. Muller. 2008. Fluorescence correlation spectroscopy of finite-sized particles. Biophys. J. 94:2800-2808.
27. Olmstead, S. 2004. Study of cotransfection and coexpression in cells. MS thesis. University of Minnesota/Twin Cities.
28. Larson, D. R., M. C. Johnson, W. W. Webb, and V. M. Vogt. 2005. Visualization of retrovirus budding with correlated light and electron microscopy. Proc. Natl. Acad. Sci. USA. 102:15453-15458.
29. Pornillos, O., D. S. Higginson, K. M. Stray, R. D. Fisher, J. E. Garrus, et al. 2003. HIV Gag mimics the Tsg101-recruiting activity of the human Hrs protein. J. Cell Biol. 162:425-434.
30. Fuller, S. D., T. Wilk, B. E. Gowen, H. G. Krausslich, and V. M. Vogt. 1997. Cryo-electron microscopy reveals ordered domains in the immature HIV-1 particle. Curr. Biol. 7:729-738.
31. Resh, M. D. 2005. Intracellular trafficking of HIV-1 Gag: how Gag interacts with cell membranes and makes viral particles. AIDS Rev. 7:84-91.
32. Demirov, D. G., and E. O. Freed. 2004. Retrovirus budding. Virus Res. 106:87-102.
33. Yap, M. W., and J. P. Stoye. 2003. Bending out and breaking away: host-cell accomplices in retroviral escape. J. Biol. 3:3.
34. Briggs, J. A., T. Wilk, R. Welker, H. G. Krausslich, and S. D. Fuller. 2003. Structural organization of authentic, mature HIV-1 virions and cores. EMBO J. 22:1707-1715.
35. Li, S., C. P. Hill, W. I. Sundquist, and J. T. Finch. 2000. Image reconstructions of helical assemblies of the HIV-1 CA protein. Nature. 407:409-413.
36. Nguyen, T. T., R. F. Bruinsma, and W. M. Gelbart. 2006. Continuum theory of retroviral capsids. Phys. Rev. Lett. 96:078102.