Transcription analysis of the extracellular adherence protein from staphylococcus aureus in authentic human infection and in vitro

Journal of Infectious Diseases

Volumn 199, Issue 10, 2009, Pages 1471-1478

  Joost, Insa ^a   Blass, Daniel ^a   Burian, Marc ^b   Goerke, Christiane ^b   Wolz, Christiane ^b   Müller, Lutz Von ^a   Becker, Karsten ^c   Preissner, Klaus ^d   Herrmann, Mathias ^a   Bischoff, Markus ^a  

^a SAARLAND UNIVERSITY   (Germany)

^b UNIVERSITY HOSPITAL TÜBINGEN   (Germany)

^c UNIVERSITY HOSPITAL MÜNSTER   (Germany)

^d JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL PROTEIN; DNA TOPOISOMERASE (ATP HYDROLYSING) B; STAPHYLOCOCCAL EXTRACELLULAR ADHERENCE PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; BACTERIUM ISOLATE; BLOOD CULTURE; CLINICAL ARTICLE; CONTROLLED STUDY; GENE EXPRESSION; GENETIC TRANSCRIPTION; HUMAN; HUMAN TISSUE; NONHUMAN; PRIORITY JOURNAL; STAPHYLOCOCCUS AUREUS; STAPHYLOCOCCUS INFECTION; WOUND HEALING IMPAIRMENT; WOUND INFECTION;

ALGORITHMS; BACTERIAL PROTEINS; DNA PRIMERS; DNA, BACTERIAL; DNA, COMPLEMENTARY; HUMANS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA-BINDING PROTEINS; STAPHYLOCOCCAL INFECTIONS; STAPHYLOCOCCUS AUREUS; TRANSCRIPTION, GENETIC; WOUNDS AND INJURIES;

EID: 65549097794     PISSN: 00221899     EISSN: None     Source Type: Journal    
DOI: 10.1086/598484     Document Type: Article

References (46)

1. Lowy FD. Staphylococcus aureus infections. N Engl J Med 1998; 339: 520-32.
2. Gjodsbol K, Christensen JJ, Karlsmark T, Jorgensen B, Klein BM, Krogfelt KA. Multiple bacterial species reside in chronic wounds: a longitudinal study. Int Wound J 2006; 3:225-31.
3. Emori TG, Gaynes RP. An overview of nosocomial infections, including the role of the microbiology laboratory. Clin Microbiol Rev 1993; 6: 428-42.
4. Lipsky BA, Berendt AR, Deery HG, et al. Diagnosis and treatment of diabetic foot infections. Plast Reconstr Surg 2006; 117:212S-38S.
5. Foster TJ. Immune evasion by staphylococci. Nat Rev Microbiol 2005; 3:948-58.
6. Chavakis T, Preissner KT, Herrmann M. The anti-inflammatory activities of Staphylococcus aureus. Trends Immunol 2007; 28:408-18.
7. Chavakis T, Wiechmann K, Preissner KT, Herrmann M. Staphylococcus aureus interactions with the endothelium: the role of bacterial "secretable expanded repertoire adhesive molecules" (SERAM) in disturbing host defense systems. Thromb Haemost 2005; 94:278-85.
8. Harraghy N, Hussain M, Haggar A, et al. The adhesive and immunomodulating properties of the multifunctional Staphylococcus aureus protein Eap. Microbiology 2003; 149:2701-7.
9. Hussain M, von Eiff C, Sinha B, et al. eap gene as novel target for specific identification of Staphylococcus aureus. J Clin Microbiol 2008; 46:470-6.
10. Johnson M, Cockayne A, Morrissey JA. Iron-regulated biofilm formation in Staphylococcus aureus Newman requires ica and the secreted protein Emp. Infect Immun 2008; 76:1756-65.
11. Scriba TJ, Sierro S, Brown EL, Phillips RE, Sewell AK, Massey RC. The Staphylococcus aureus Eap protein activates expression of proinflammatory cytokines. Infect Immun 2008; 76:2164-8.
12. Schneider D, Liaw L, Daniel C, et al. Inhibition of breast cancer cell adhesion and bone metastasis by the extracellular adherence protein of Staphylococcus aureus. Biochem Biophys Res Commun 2007; 357:282-8.
13. Athanasopoulos AN, Economopoulou M, Orlova VV, et al. The extracellular adherence protein (Eap) of Staphylococcus aureus inhibits wound healing by interfering with host defense and repair mechanisms. Blood 2006; 107:2720-7.
14. Hansen U, Hussain M, Villone D, et al. The anchorless adhesin Eap (extracellular adherence protein) from Staphylococcus aureus selectively recognizes extracellular matrix aggregates but binds promiscuously to monomeric matrix macromolecules. Matrix Biol 2006; 25:252-60.
15. Sobke AC, Selimovic D, Orlova V, et al. The extracellular adherence protein from Staphylococcus aureus abrogates angiogenic responses of endothelial cells by blocking Ras activation. FASEB J 2006; 20:2621-3.
16. Xie C, Alcaide P, Geisbrecht BV, et al. Suppression of experimental autoimmune encephalomyelitis by extracellular adherence protein of Staphylococcus aureus. J Exp Med 2006; 203:985-94.
17. McGavin MH, Krajewska-Pietrasik D, Ryden C, Hook M. Identification of a Staphylococcus aureus extracellular matrix-binding protein with broad specificity. Infect Immun 1993; 61:2479-85.
18. Palma M, Haggar A, Flock JI. Adherence of Staphylococcus aureus is enhanced by an endogenous secreted protein with broad binding activity. J Bacteriol 1999; 181:2840-5.
19. Kreikemeyer B, McDevitt D, Podbielski A. The role of the Map protein in Staphylococcus aureus matrix protein and eukaryotic cell adherence. Int J Med Microbiol 2002; 292:283-95.
20. Haggar A, Hussain M, Lonnies H, Herrmann M, Norrby-Teglund A, Flock JI. Extracellular adherence protein from Staphylococcus aureus enhances internalization into eukaryotic cells. Infect Immun 2003; 71:2310-7.
21. Hussain M, Haggar A, Heilmann C, Peters G, Flock JI, Herrmann M. Insertional inactivation of Eap in Staphylococcus aureus strain Newman confers reduced staphylococcal binding to fibroblasts. Infect Immun 2002; 70:2933-40.
22. Chavakis T, Hussain M, Kanse SM, et al. Staphylococcus aureus extracellular adherence protein serves as anti-inflammatory factor by inhibiting the recruitment of host leukocytes. Nat Med 2002; 8:687-93.
23. Haggar A, Ehrnfelt C, Holgersson J, Flock JI. The extracellular adherence protein from Staphylococcus aureus inhibits neutrophil binding to endothelial cells. Infect Immun 2004; 72:6164-7.
24. Lee LY, Miyamoto YJ, McIntyre BW, et al. The Staphylococcus aureus Map protein is an immunomodulator that interferes with T cellmediated responses. J Clin Invest 2002; 110:1461-71.
25. Bjarnsholt T, Kirketerp-Moller K, Jensen PO, et al.Whychronic wounds will not heal: a novel hypothesis. Wound Repair Regen 2008; 16:2-10.
26. James GA, Swogger E, Wolcott R, et al. Biofilms in chronic wounds. Wound Repair Regen 2008; 16:37-44.
27. Goerke C, Campana S, Bayer MG, Doring G, Botzenhart K, Wolz C. Direct quantitative transcript analysis of the agr regulon of Staphylococcus aureus during human infection in comparison to the expression profile in vitro. Infect Immun 2000; 68:1304-11.
28. Goerke C, Fluckiger U, Steinhuber A, Zimmerli W, Wolz C. Impact of the regulatory loci agr, sarA and sae of Staphylococcus aureus on the induction of alpha-toxin during device-related infection resolved by direct quantitative transcript analysis. Mol Microbiol 2001; 40:1439-47.
29. Goerke C, Bayer MG, Wolz C. Quantification of bacterial transcripts during infection using competitive reverse transcription-PCR (RTPCR) and LightCycler RT-PCR. Clin Diagn Lab Immunol 2001; 8:279-82.
30. Goerke C, Wolz C. Regulatory and genomic plasticity of Staphylococcus aureus during persistent colonization and infection. Int J Med Microbiol 2004; 294:195-202.
31. Goerke C, Fluckiger U, Steinhuber A, et al. Role of Staphylococcus aureus global regulators sae and sigma B in virulence gene expression during device-related infection. Infect Immun 2005; 73:3415-21.
32. Wolz C, Goerke C, Landmann R, Zimmerli W, Fluckiger U. Transcription of clumping factor A in attached and unattached Staphylococcus aureus in vitro and during device-related infection. Infect Immun 2002; 70:2758-62.
33. Wright JS III, Jin R, Novick RP. Transient interference with staphylococcal quorum sensing blocks abscess formation. Proc Natl Acad Sci USA 2005; 102:1691-6.
34. FDA Wound Healing Clinical Focus Group. Guidance for industry: chronic cutaneous ulcer and burn wounds-developing products for treatment. Wound Repair Regen 2001; 9:258-68.
35. Harmsen D, Claus H, Witte W, et al. Typing of methicillin-resistant Staphylococcus aureus in a university hospital setting by using novel software for spa repeat determination and database management. J Clin Microbiol 2003; 41:5442-8.
36. Harraghy N, Kormanec J, Wolz C, et al. sae is essential for expression of the staphylococcal adhesins Eap and Emp. Microbiology 2005; 151:1789-800.
37. Hussain M, Becker K, von Eiff C, Peters G, Herrmann M. Analogs of Eap protein are conserved and prevalent in clinical Staphylococcus aureus isolates. Clin Diagn Lab Immunol 2001; 8:1271-6.
38. Boden MK, Flock JI. Evidence for three different fibrinogen-binding proteins with unique properties from Staphylococcus aureus strain Newman. Microb Pathog 1992; 12:289-98.
39. Dunman PM, Murphy E, Haney S, et al. Transcription profiling-based identification of Staphylococcus aureus genes regulated by the agr and/or sarA loci. J Bacteriol 2001; 183:7341-53.
40. Buckling A, Neilson J, Lindsay J, et al. Clonal distribution and phasevariable expression of a major histocompatibility complex analogue protein in Staphylococcus aureus. J Bacteriol 2005; 187:2917-9.
41. Steinhuber A, Landmann R, Goerke C, Wolz C, Fluckiger U. Bioluminescence imaging to study the promoter activity of hla of Staphylococcus aureus in vitro and in vivo. Int J Med Microbiol 2008; 298:599-605.
42. Perel P, Roberts I, Sena E, et al. Comparison of treatment effects between animal experiments and clinical trials: systematic review. BMJ 2007; 334:197.
43. Davis HL. Novel vaccines and adjuvant systems: the utility of animal models for predicting immunogenicity in humans. Hum Vaccin 2007; 4:73-7.
44. Zimmerli W, Waldvogel FA, Vaudaux P, Nydegger UE. Pathogenesis of foreign body infection: description and characteristics of an animal model. J Infect Dis 1982; 146:487-97.
45. Lucet JC, Herrmann M, Rohner P, Auckenthaler R, Waldvogel FA, Lew DP. Treatment of experimental foreign body infection caused by methicillin-resistant Staphylococcus aureus. Antimicrob Agents Chemother 1990; 34:2312-7.
46. Geiger T, Goerke C, Mainiero M, Kraus D, Wolz C. The virulence regulator Sae of Staphylococcus aureus: promoter activities and response to phagocytosis-related signals. J Bacteriol 2008; 190:3419-28.