Chapter 17 Determination of Yeast Mitochondrial KHE Activity, Osmotic Swelling and Mitophagy

Methods in Enzymology

Volumn 457, Issue B, 2009, Pages 305-317

  Nowikovsky, Karin ^a   Devenish, Rodney J ^b   Froschauer, Elisabeth ^a   Schweyen, Rudolf J ^a  

^a UNIVERSITY OF VIENNA   (Austria)

^b MONASH UNIVERSITY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN; POTASSIUM ION; POTASSIUM PROTON EXCHANGE PROTEIN; PROTEIN MDM38P; UNCLASSIFIED DRUG; CARRIER PROTEIN; POTASSIUM; SACCHAROMYCES CEREVISIAE PROTEIN; YOL027C PROTEIN, S CEREVISIAE;

AUTOPHAGY; DOWN REGULATION; ENZYME ACTIVATION; MITOCHONDRION; MITOPHAGY; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; REVIEW; SWELLING; YEAST; ARTICLE; FLUORESCENCE MICROSCOPY; GENE EXPRESSION REGULATION; GENETIC PROCEDURES; GENETICS; GROWTH, DEVELOPMENT AND AGING; METABOLISM; METHODOLOGY; MITOCHONDRIAL MEMBRANE POTENTIAL; OSMOSIS; SACCHAROMYCES CEREVISIAE;

AUTOPHAGY; BIOSENSING TECHNIQUES; GENE EXPRESSION REGULATION; MEMBRANE POTENTIAL, MITOCHONDRIAL; MEMBRANE PROTEINS; MICROSCOPY, FLUORESCENCE; MITOCHONDRIA; MITOCHONDRIAL MEMBRANE TRANSPORT PROTEINS; OSMOSIS; POTASSIUM; POTASSIUM-HYDROGEN ANTIPORTERS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS;

EID: 65449189191     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(09)05017-4     Document Type: Review

References (19)

1. Bevis B.J., and Glick B.S. Rapidly maturing variants of the Discosoma red fluorescent protein (DsRed). Nat. Biotechnol. 20 (2002) 83-87
2. + exchange in mitochondrial membrane vesicles involves Yol027/Letm1 proteins. Biochim. Biophys. Acta 1711 (2005) 41-48
3. + exchanger. J. Biol. Chem. 255 (1980) 11273-11279
4. Gietz R.D., and Woods R.A. Transformation of yeast by lithium acetate/singlestranded carrier DNA/polyethylene glycol method. Methods Enzymol. 350 (2002) 87-96
5. Heikal A.A., Hess S.T., Baird G.S., Tsien R.Y., and Webb W.W. Molecular spectroscopy and dynamics of intrinsically fluorescent proteins: Coral red (dsRed) and yellow (Citrine). Proc. Natl. Acad. Sci. USA 97 (2000) 11996-12001
6. Klebe R.J., Harriss J.V., Sharp Z.D., and Douglas M.G. A general method for polyethylene-glycol-induced genetic transformation of bacteria and yeast. Gene 25 (1983) 333-341
7. + exchange in yeast mitochondria: Sensitivity to inhibitors, solubilization and reconstitution of the activity in proteoliposomes. Biochim. Biophys. Acta 1108 (1992) 169-176
8. Mitchell P., and Moyle J. Chemiosmotic hypothesis of oxidative phosphorylation. Nature 213 (1967) 137-139
9. + exchange activity, osmotic swelling and mitophagy. Cell Death Differ. 14 (2007) 1647-1656
10. Nowikovsky K., Schweyen R.J., and Bernardi P. Pathophysiology of mitochondrial volume homeostasis: Potassium transport and permeability transition. Biochim. Biophys. Acta. (2009) (In press)
11. Ohkuma S., and Poole B. Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH by various agents. Proc. Natl. Acad. Sci. USA 75 (1978) 3327-3331
12. Preston R.A., Murphy R.F., and Jones E.W. Assay of vacuolar pH in yeast and identification of acidification-defective mutants. Proc. Natl. Acad. Sci. USA 86 (1989) 7027-7031
13. Reers M., Smiley S.T., Mottola-Hartshorn C., Chen A., Lin M., and Chen L.B. Mitochondrial membrane potential monitored by JC-1 dye. Methods Enzymol. 260 (1995) 406-417
14. Rosado C.J., Mijaljica D., Hatzinisiriou I., Prescott M., and Devenish R.J. Rosella: A fluorescent pH-biosensor for reporting vacuolar turnover of cytosol and organelles in yeast. Autophagy 4 (2008) 205-213
15. Sherman F. Getting started with yeast. Methods Enzymol. 194 (1991) 3-21
16. Vida T.A., and Emr S.D. A new vital stain for visualizing vacuolar membrane dynamics and endocytosis in yeast. J. Cell Biol 128 (1995) 779-792
17. Westermann B., and Neupert W. Mitochondria-targeted green fluorescent proteins: Convenient tools for the study of organelle biogenesis in Saccharomyces cerevisiae. Yeast 16 (2000) 1421-1427
18. Yoshimori T., Yamamoto A., Moriyama Y., Futai M., and Tashiro Y. Bafilomycin A1, a specific inhibitor of vacuolar-type H(+)-ATPase, inhibits acidification and protein degradation in lysosomes of cultured cells. J. Biol. Chem. 266 (1991) 17707-17712
19. Zinser E., and Daum G. Isolation and biochemical characterization of organelles from the yeast, Saccharomyces cerevisiae. Yeast 11 (1995) 493-536