Chapter 10 Detection of a Mitochondrial Kinase Complex That Mediates PKA-MEK-ERK-Dependent Phosphorylation of Mitochondrial Proteins Involved in the Regulation of Steroid Biosynthesis

Methods in Enzymology

Volumn 457, Issue B, 2009, Pages 169-192

  Paz, Cristina ^a   Poderoso, Cecilia ^a   Maloberti, Paula ^a   Maciel, Fabiana Cornejo ^a   Mendez, Carlos ^a   Poderoso, Juan J ^a   Podestá, Ernesto J ^a  

^a UNIVERSIDAD DE BUENOS AIRES   (Argentina)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIC AMP DEPENDENT PROTEIN KINASE; MITOCHONDRIAL PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; MITOGEN ACTIVATED PROTEIN KINASE KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE KINASE 2; PALMITOYL COENZYME A HYDROLASE; PHOSPHOPROTEIN; MITOGEN ACTIVATED PROTEIN KINASE KINASE; PROTEIN KINASE; STEROID; THIOL ESTER HYDROLASE;

CELL DISRUPTION; CONFOCAL MICROSCOPY; ENZYME ISOLATION; ENZYME LOCALIZATION; ENZYME PURIFICATION; ENZYME SUBSTRATE COMPLEX; HUMAN; MITOCHONDRION; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; REVIEW; STEROIDOGENESIS; WESTERN BLOTTING; ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ENZYMOLOGY; GENETIC TRANSFECTION; GENETICS; METABOLISM; MUTATION; PHOSPHORYLATION; PROTEIN PROCESSING;

ANIMALS; CYCLIC AMP-DEPENDENT PROTEIN KINASES; HUMANS; MITOCHONDRIA; MITOGEN-ACTIVATED PROTEIN KINASE KINASES; MUTATION; PHOSPHORYLATION; PROTEIN KINASES; PROTEIN PROCESSING, POST-TRANSLATIONAL; STEROIDS; THIOLESTER HYDROLASES; TRANSFECTION;

EID: 65449169284     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(09)05010-1     Document Type: Review

References (31)

1. Al-Hakim A., Rui X., Tsao J., Albert P.R., and Schimmer B.P. Forskolin-resistant Y1 adrenal cell mutants are deficient in adenylyl cyclase type 4. Mol. Cell. Endocrinol. 214 (2004) 155-165
2. Angelo R.G., and Rubin C.S. Characterization of structural features that mediate the tethering of Caenorhabditis elegans protein kinase A to a novel a kinase anchor protein. Insights into the anchoring of PKAI isoforms. J. Biol. Chem. 275 (2000) 4351-4362
3. Ascoli M. Characterization of several clonal lines of cultured Leydig tumor cells: Gonadotropin receptors and steroidogenic responses. Endocrinology 108 (1981) 88-95
4. Bardwell A.J., Abdollahi M., and Bardwell L. Docking sites on mitogen-activated protein kinase (MAPK) kinases, MAPK phosphatases and the Elk-1 transcription factor compete for MAPK binding and are crucial for enzymic activity. Biochem. J. 370 (2003) 1077-1085
5. Castilla R., Gadaleta M., Castillo A.F., Duarte A., Neuman I., Paz C., Cornejo Maciel F., and Podesta E.J. New enzymes involved in the mechanism of action of epidermal growth factor in a clonal strain of Leydig tumor cells. Endocrinology 149 (2008) 3743-3752
6. Castillo F., Cornejo Maciel F., Castilla R., Duarte A., Maloberti P., Paz C., and Podesta E.J. cAMP increases mitochondrial cholesterol transport through the induction of arachidonic acid release inside this organelle in MA-10 Leydig cells. FEBS J. 273 (2006) 5011-5021
7. Cornejo Maciel F., Maloberti P., Neuman I., Cano F., Castilla R., Castillo F., Paz C., and Podesta E.J. An arachidonic acid-preferring acyl-CoA synthetase is a hormone-dependent and obligatory protein in the signal transduction pathway of steroidogenic hormones. J. Mol. Endocrinol. 34 (2005) 655-666
8. Dada L.A., Paz C., Mele P., Solano A.R., Cornejo Maciel F., and Podesta E.J. The cytosol as site of phosphorylation of the cyclic AMP-dependent protein kinase in adrenal steroidogenesis. J. Steroid Biochem. Mol. Biol. 39 (1991) 889-896
9. Diviani D., and Scott J.D. AKAP signaling complexes at the cytoskeleton. J. Cell Sci. 114 (2001) 1431-1437
10. Fantz D.A., Jacobs D., Glossip D., and Kornfeld K. Docking sites on substrate proteins direct extracellular signal-regulated kinase to phosphorylate specific residues. J. Biol. Chem. 276 (2001) 27256-27265
11. Feliciello A., Gottesman M.E., and Avvedimento E.V. The biological functions of A-kinase anchor proteins. J. Mol. Biol. 308 (2001) 99-114
12. Finkielstein C., Maloberti P., Mendez C.F., Paz C., Cornejo Maciel F., Cymeryng C., Neuman I., Dada L., Mele P.G., Solano A., and Podesta E.J. An adrenocorticotropin-regulated phosphoprotein intermediary in steroid synthesis is similar to an acyl-CoA thioesterase enzyme. Eur. J. Biochem. 256 (1998) 60-66
13. Gerber L.K., Aronow B.J., and Matlib M.A. Activation of a novel long-chain free fatty acid generation and export system in mitochondria of diabetic rat hearts. Am. J. Physiol. Cell Physiol. 291 (2006) C1198-C1207
14. Hunt M.C., and Alexson S.E. The role Acyl-CoA thioesterases play in mediating intracellular lipid metabolism. Prog. Lipid Res. 41 (2002) 99-130
15. Lin D., Sugawara T., Strauss III J.F., Clark B.J., Stocco D.M., Saenger P., Rogol A., and Miller W.L. Role of steroidogenic acute regulatory protein in adrenal and gonadal steroidogenesis. Science 267 (1995) 1828-1831
16. Lindquist P.J., Svensson L.T., and Alexson S.E. Molecular cloning of the peroxisome proliferator-induced 46-kDa cytosolic acyl-CoA thioesterase from mouse and rat liver-Recombinant expression in Escherichia coli, tissue expression, and nutritional regulation. Eur. J. Biochem. 251 (1998) 631-640
17. Maloberti P., Castilla R., Castillo F., Maciel F.C., Mendez C.F., Paz C., and Podesta E.J. Silencing the expression of mitochondrial acyl-CoA thioesterase I and acyl-CoA synthetase 4 inhibits hormone-induced steroidogenesis. FEBS J. 272 (2005) 1804-1814
18. Maloberti P., Lozano R.C., Mele P.G., Cano F., Colonna C., Mendez C.F., Paz C., and Podesta E.J. Concerted regulation of free arachidonic acid and hormone-induced steroid synthesis by acyl-CoA thioesterases and acyl-CoA synthetases in adrenal cells. Eur. J. Biochem. 269 (2002) 5599-5607
19. Maloberti P., Mele P.G., Neuman I., Cornejo Maciel F., Cano F., Bey P., Paz C., and Podesta E.J. Regulation of arachidonic acid release in steroidogenesis: Role of a new acyl-CoA thioestrase (ARTISt). Endocr. Res. 26 (2000) 653-662
20. Mele P.G., Dada L.A., Paz C., Neuman I., Cymeryng C.B., Mendez C.F., Finkielstein C.V., Cornejo Maciel F., and Podesta E.J. Involvement of arachidonic acid and the lipoxygenase pathway in mediating luteinizing hormone-induced testosterone synthesis in rat Leydig cells. Endocr. Res. 23 (1997) 15-26
21. Neher R., Milani A., Solano A.R., and Podesta E.J. Compartmentalization of corticotropin-dependent steroidogenic factors in adrenal cortex: Evidence for a post-translational cascade in stimulation of the cholesterol side-chain split. Proc. Natl. Acad. Sci. USA 79 (1982) 1727-1731
22. Paz C., Dada L., Cornejo Maciel F., Mele P., Cymeryng C., Neuman I., Mendez C., Finkielstein C., Solano A., Minkiyu P., Fisher W.H., Towbin H., et al. Purification of a novel 43-kDa protein (p43) intermediary in the activation of steroidogenesis from rat adrenal gland. Eur. J. Biochem. 224 (1994) 709-716
23. Poderoso C., Converso D.P., Maloberti P., Duarte A., Neuman I., Galli S., Maciel F.C., Paz C., Carreras M.A.C., Poderoso J.J., and Podestá E.J. A mitochondrial kinase complex is essential to mediate an ERK1/2-dependent phosphorylation of a key regulatory protein in steroid biosynthesis. PLoS ONE 3 (2008) e1443
24. Rae P.A., Gutmann N.S., Tsao J., and Schimmer B.P. Mutations in cyclic AMP-dependent protein kinase and corticotropin (ACTH)-sensitive adenylate cyclase affect adrenal steroidogenesis. Proc. Natl. Acad. Sci. USA 76 (1979) 1896-1900
25. Robinson F.L., Whitehurst A.W., Raman M., and Cobb M.H. Identification of novel point mutations in ERK2 that selectively disrupt binding to MEK1. J. Biol. Chem. 277 (2002) 14844-14852
26. Rubin C.S. A kinase anchor proteins and the intracellular targeting of signals carried by cyclic AMP. Biochim. Biophys. Acta 1224 (1994) 467-479
27. Schagger H., and von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166 (1987) 368-379
28. Schimmer B.P., Tsao J., and Knapp M. Isolation of mutant adrenocortical tumor cells resistant to cyclic nucleotides. Mol. Cell. Endocrinol. 8 (1977) 135-145
29. Stocco D.M., and Clark B.J. Regulation of the acute production of steroids in steroidogenic cells. Endocr. Rev. 17 (1996) 221-244
30. Svensson L.T., Engberg S.T., Aoyama T., Usuda N., Alexson S.E., and Hashimoto T. Molecular cloning and characterization of a mitochondrial peroxisome proliferator-induced acyl-CoA thioesterase from rat liver. Biochem. J. 329 Pt 3 (1998) 601-608
31. Yamada J., Hamuro J., Sano Y., Maruyama K., and Kinoshita S. Allogeneic corneal tolerance in rodents with long-term graft survival. Transplantation 79 (2005) 1362-1369