Chapter 22 Methods for Assessing and Modulating UCP2 Expression and Function

Methods in Enzymology

Volumn 457, Issue B, 2009, Pages 395-404

  Velloso, Lício A ^a   Degasperi, Giovanna R ^a   Vercesi, Aníbal E ^a   Saad, Mário A ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

[No Author keywords available]

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA COACTIVATOR 1ALPHA; UNCOUPLING PROTEIN 2; ION CHANNEL; MITOCHONDRIAL PROTEIN; MITOCHONDRIAL UNCOUPLING PROTEIN 2; OXYGEN; PEROXISOME PROLIFERATOR ACTIVATED RECEPTOR GAMMA COACTIVATOR 1, RAT; PEROXISOME PROLIFERATOR-ACTIVATED RECEPTOR GAMMA COACTIVATOR-1, RAT; RNA BINDING PROTEIN; TRANSCRIPTION FACTOR;

ANIMAL TISSUE; COLD EXPOSURE; CONTROLLED STUDY; FATTY ACID TRANSPORT; GENE REPRESSION; HYPOTHALAMUS; IMMUNOBLOTTING; INSULIN RELEASE; MALE; MITOCHONDRIAL RESPIRATION; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATIVE STRESS; PANCREAS ISLET CELL; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN FUNCTION; RAT; REAL TIME POLYMERASE CHAIN REACTION; REVIEW; ANIMAL; ARTICLE; COLD; GENE EXPRESSION REGULATION; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; MITOCHONDRION; MOUSE; NUCLEIC ACID PROBE; PANCREAS ISLET; POLYMERASE CHAIN REACTION; WISTAR RAT;

ANIMALIA;

ANIMALS; ANTISENSE ELEMENTS (GENETICS); COLD TEMPERATURE; GENE EXPRESSION REGULATION; HYPOTHALAMUS; IMMUNOBLOTTING; ION CHANNELS; ISLETS OF LANGERHANS; MALE; MICE; MITOCHONDRIA; MITOCHONDRIAL PROTEINS; OXYGEN; POLYMERASE CHAIN REACTION; RATS; RATS, WISTAR; RNA-BINDING PROTEINS; TRANSCRIPTION FACTORS;

EID: 65449131205     PISSN: 00766879     EISSN: None     Source Type: Book Series    
DOI: 10.1016/S0076-6879(09)05022-8     Document Type: Review

References (19)

1. Bradford M.M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72 (1976) 248-254
2. Brand M.D., Affourtit C., Esteves T.C., Green K., Lambert A.J., Miwa S., Pakay J.L., and Parker N. Mitochondrial superoxide: Production, biological effects, and activation of uncoupling proteins. Free Radic. Biol. Med. 37 (2004) 755-767
3. Brand M.D., and Esteves T.C. Physiological functions of the mitochondrial uncoupling proteins UCP2 and UCP3. Cell Metab. 2 (2005) 85-93
4. Cannon B., Shabalina I.G., Kramarova T.V., Petrovic N., and Nedergaard J. Uncoupling proteins: A role in protection against reactive oxygen species-or not?. Biochim. Biophys. Acta 1757 (2006) 449-458
5. Chance B., and Williams G.R. Respiratory enzymes in oxidative phosphorylation. VI. The effects of adenosine diphosphate on azide-treated mitochondria. J. Biol. Chem. 221 (1956) 477-489
6. Degasperi G.R., Romanatto T., Denis R.G., Araujo E.P., Moraes J.C., Inada N.M., Vercesi A.E., and Velloso L.A. UCP2 protects hypothalamic cells from TNF-alpha-induced damage. FEBS Lett. 582 (2008) 3103-3110
7. De Souza C.T., Araujo E.P., Prada P.O., Saad M.J., Boschero A.C., and Velloso L.A. Short-term inhibition of peroxisome proliferator-activated receptor-gamma coactivator-1alpha expression reverses diet-induced diabetes mellitus and hepatic steatosis in mice. Diabetologia 48 (2005) 1860-1871
8. De Souza C.T., Araujo E.P., Stoppiglia L.F., Pauli J.R., Ropelle E., Rocco S.A., Marin R.M., Franchini K.G., Carvalheira J.B., Saad M.J., Boschero A.C., Carneiro E.M., et al. Inhibition of UCP2 expression reverses diet-induced diabetes mellitus by effects on both insulin secretion and action. FASEB J. 21 (2007) 1153-1163
9. De Souza C.T., Gasparetti A.L., Pereira-da-Silva M., Araujo E.P., Carvalheira J.B., Saad M.J., Boschero A.C., Carneiro E.M., and Velloso L.A. Peroxisome proliferator-activated receptor gamma coactivator-1-dependent uncoupling protein-2 expression in pancreatic islets of rats: A novel pathway for neural control of insulin secretion. Diabetologia 46 (2003) 1522-1531
10. Esteves T.C., and Brand M.D. The reactions catalysed by the mitochondrial uncoupling proteins UCP2 and UCP3. Biochim. Biophys. Acta 1709 (2005) 35-44
11. Handschin C., and Spiegelman B.M. Peroxisome proliferator-activated receptor gamma coactivator 1 coactivators, energy homeostasis, and metabolism. Endocr. Rev. 27 (2006) 728-735
12. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 (1970) 680-685
13. Oliveira R.L., Ueno M., de Souza C.T., Pereira-da-Silva M., Gasparetti A.L., Bezzera R.M., Alberici L.C., Vercesi A.E., Saad M.J., and Velloso L.A. Cold-induced PGC-1alpha expression modulates muscle glucose uptake through an insulin receptor/Akt-independent, AMPK-dependent pathway. Am. J. Physiol. Endocrinol. Metab. 287 (2004) E686-E695
14. Reynafarje B., Costa L.E., and Lehninger A.L. O2 solubility in aqueous media determined by a kinetic method. Anal. Biochem. 145 (1985) 406-418
15. Simoneau J.A., Kelley D.E., Neverova M., and Warden C.H. Overexpression of muscle uncoupling protein 2 content in human obesity associates with reduced skeletal muscle lipid utilization. FASEB J. 12 (1998) 1739-1745
16. Sims N.R. Rapid isolation of metabolically active mitochondria from rat brain and subregions using Percoll density gradient centrifugation. J. Neurochem. 55 (1990) 698-707
17. Sims N.R., and Blass J.P. Expression of classical mitochondrial respiratory responses in homogenates of rat forebrain. J. Neurochem. 47 (1986) 496-505
18. van Suylichem P.T., Wolters G.H., and van Schilfgaarde R. The efficacy of density gradients for islet purification: A comparison of seven density gradients. Transpl. Int. 3 (1990) 156-161
19. Zhang C.Y., Baffy G., Perret P., Krauss S., Peroni O., Grujic D., Hagen T., Vidal-Puig A.J., Boss O., Kim Y.B., Zheng X.X., Wheeler M.B., et al. Uncoupling protein-2 negatively regulates insulin secretion and is a major link between obesity, beta cell dysfunction, and type 2 diabetes. Cell 105 (2001) 745-755