Cellular internalization of arginine-rich peptides into tobacco suspension cells: A structure-activity relationship study

Journal of Peptide Science

Volumn 15, Issue 4, 2009, Pages 259-263

  Mizuno, Takashi ^a   Miyashita, Masahiro ^a   Miyagawa, Hisashi ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Arginine rich peptide; Cell wall; Cell penetrating peptide; Fluorescence; Plant cell

Indexed keywords

ARGININE RICH PEPTIDE; CELL PENETRATING PEPTIDE; TRYPSIN; UNCLASSIFIED DRUG; 3',6' DIACETYLFLUORESCEIN; 3',6'-DIACETYLFLUORESCEIN; ARGININE; FLUORESCEIN DERIVATIVE; FLUORESCENT DYE; OLIGOPEPTIDE;

ADSORPTION; AMINO ACID SEQUENCE; ARTICLE; CELL SUSPENSION; CELL WALL; CHEMICAL LABELING; CYTOPLASM; INTERNALIZATION; MACROMOLECULE; MICROSCOPY; NONHUMAN; PLANT CELL; PRIORITY JOURNAL; PROTEIN STRUCTURE; PROTEIN SYNTHESIS; SEQUENCE ANALYSIS; SPECTROFLUOROMETRY; STRUCTURE ACTIVITY RELATION; TOBACCO; ACTIVE TRANSPORT; CELL CULTURE; CHEMISTRY; DRUG DELIVERY SYSTEM; FLUORESCENCE MICROSCOPY; METABOLISM; SYNTHESIS;

NICOTIANA TABACUM;

ARGININE; BIOLOGICAL TRANSPORT, ACTIVE; CELL WALL; CELLS, CULTURED; DRUG DELIVERY SYSTEMS; FLUORESCEINS; FLUORESCENT DYES; MICROSCOPY, FLUORESCENCE; OLIGOPEPTIDES; SPECTROMETRY, FLUORESCENCE; STRUCTURE-ACTIVITY RELATIONSHIP; TOBACCO;

EID: 65349084336     PISSN: 10752617     EISSN: 10991387     Source Type: Journal    
DOI: 10.1002/psc.1079     Document Type: Article

References (18)

1. Dietz GP, Bähr M. Delivery of bioactive molecules into the cell: the Trojan horse approach. Mol. Cell. Neurosci. 2004; 27: 85-131.
2. Vivès E, Brodin P, Lebleu B. A truncated HIV-1 Tat protein basic domain rapidly translocates through the plasma membrane and accumulates in the cell nucleus. J. Biol. Chem. 1997; 272: 16010-16017.
3. Derossi D, Joliot AH, Chassaing G, Prochiantz A. The third helix of the Antennapedia homeodomain translocates through biological membranes. J. Biol. Chem. 1994; 269: 10444-10450.
4. Elmquist A, Lindgren M, Bartfai T, Langel Ü. VE-cadherin-derived cell-penetrating peptide, pVEC, with carrier functions. Exp. Cell Res. 2001; 269: 237-244.
5. Mitchell DJ, Kim DT, Steinman L, Fathman CG, Rothbard JB. Polyarginine enters cells more efficiently than other polycationic homopolymers. J. Peptide Res. 2000; 56: 318-325.
6. Futaki S, Suzuki T, Ohashi W, Yagami T, Tanaka S, Ueda K, Sugiura Y. Arginine-rich peptides. An abundant source of membrane-permeable peptides having potential as carriers for intracellular protein delivery. J. Biol. Chem. 2001; 276: 5836-5840.
7. Duchardt F, Fotin-MleczekM, Schwarz H, Fischer R, Brock R. A comprehensive model for the cellular uptake of cationic cell-penetrating peptides. Traffic 2007; 8: 848-866.
8. Nakase I, Takeuchi T, Tanaka G, Futaki S. Methodological and cellular aspects that govern the internalization mechanisms of arginine-rich cell-penetrating peptides. Adv. Drug Delivery Rev. 2008; 60: 598-607.
9. Chang M, Chou JC, Lee HJ. Cellular internalization of fluorescent proteins via arginine-rich intracellular delivery peptide in plant cells. Plant Cell Physiol. 2005; 46: 482-488.
10. Chugh A, Eudes F. Translocation and nuclear accumulation of monomer and dimer of HIV-1 Tat basic domain in triticale mesophyll protoplasts. Biochim. Biophys. Acta 2007; 1768: 419-426.
11. Chugh A, Eudes F. Cellular uptake of cell-penetrating peptides pVEC and transportan in plants. J. Peptide Sci. 2008; 14: 477-481.
12. Unnamalai N, Kang BG, Lee WS. Cationic oligopeptide-mediated delivery of dsRNA for post-transcriptional gene silencing in plant cells. FEBS Lett. 2004; 566: 307-310.
13. Kosuge M, Takeuchi T, Nakase I, Jones AT, Futaki S. Cellular internalization and distribution of arginine-rich peptides as a function of extracellular peptide concentration, serum, and plasma membrane associated proteoglycans. Bioconjugate Chem. 2008; 19: 656-664.
14. Richard JP, Melikov K, Vives E, Ramos C, Verbeure B, Gait MJ, Chernomordik LV, Lebleu B. Cell-penetrating peptides. A reevaluation of the mechanism of cellular uptake. J. Biol. Chem. 2003; 278: 585-590.
15. Mäe M, Myrberg H, Jiang Y, Paves H, Valkna A, Langel Ü. Internalisation of cell-penetrating peptides into tobacco protoplasts. Biochim. Biophys. Acta 2005; 1669: 101-107.
16. El-Andaloussi S, Jarver P, Johansson HJ, Langel Ü. Cargo-dependent cytotoxicity and delivery efficacy of cell-penetrating peptides: A comparative study. Biochem. J. 2007; 407 285-292.
17. Yandek LE, Pokorny A, Floren A, Knoelke K, Langel Ü, Almeida PF. Mechanism of the cell-penetrating peptide transportan 10 permeation of lipid bilayers. Biophys. J. 2007; 92: 2434-2444.
18. Tünnemann G, Martin RM, Haupt S, Patsch C, Edenhofer F, Cardoso MC. Cargo-dependent mode of uptake and bioavailability of TAT-containing proteins and peptides in living cells. FASEB J. 2006; 20: 1775-1784.