Subcellular fractionation of human eosinophils: Isolation of functional specific granules on isoosmotic density gradients

Journal of Immunological Methods

Volumn 344, Issue 1, 2009, Pages 64-72

  Neves, Josiane S ^a   Perez, Sandra A C ^b   Spencer, Lisa A ^a   Melo, Rossana C N ^a,c   Weller, Peter F ^a  

^a BETH ISRAEL DEACONESS MEDICAL CENTER   (United States)

^b INSTITUTO OSWALDO CRUZ   (Brazil)

^c FEDERAL UNIVERSITY OF JUIZ DE FORA   (Brazil)

Author keywords

Eosinophils; Granules; Iodixanol; Isoosmotic gradient

Indexed keywords

IODIXANOL; NITROGEN;

ARTICLE; CELL FRACTIONATION; CELL GRANULE; CELL ISOLATION; CELL MEMBRANE; CONTROLLED STUDY; DENSITY GRADIENT; DENSITY GRADIENT CENTRIFUGATION; EOSINOPHIL; FLOW CYTOMETRY; HUMAN; HUMAN CELL; PRIORITY JOURNAL; STAINING; TRANSMISSION ELECTRON MICROSCOPY;

ANTIGENS, CD; BLOOD PROTEINS; CELL FRACTIONATION; CENTRIFUGATION, DENSITY GRADIENT; CYTOPLASMIC GRANULES; EOSINOPHILS; HUMANS; MICROSCOPY, ELECTRON, TRANSMISSION; PLATELET MEMBRANE GLYCOPROTEINS; PROTEOGLYCANS; SUBCELLULAR FRACTIONS; TRIIODOBENZOIC ACIDS;

EID: 65049087251     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2009.03.006     Document Type: Article

References (27)

1. Castle J.D. Purification of organelles from mammalian cells. Curr. Protoc. Immunol. (2003) 8, 8.1B, 1
2. Castle J.D. Overview of cell fractionation. Curr. Protoc. Protein Sci. (2004) 4, 4.1, 1
3. Ford T., Graham J., and Rickwood D. Iodixanol: a nonionic iso-osmotic centrifugation medium for the formation of self-generated gradients. Anal. Biochem. 220 (1994) 360
4. Gleich G.J. Mechanisms of eosinophil-associated inflammation. J. Allergy Clin. Immunol. 105 (2000) 651
5. Graham J., Ford T., and Rickwood D. The preparation of subcellular organelles from mouse liver in self-generated gradients of iodixanol. Anal. Biochem. 220 (1994) 367
6. Karawajczyk M., Seveus L., Garcia R., Bjornsson E., Peterson C.G., Roomans G.M., and Venge P. Piecemeal degranulation of peripheral blood eosinophils: a study of allergic subjects during and out of the pollen season. Am. J. Respir. Cell Mol. Biol. 23 (2000) 521
7. Kroegel C., Yukawa T., Dent G., Venge P., Chung K.F., and Barnes P.J. Stimulation of degranulation from human eosinophils by platelet-activating factor. J. Immunol. 142 (1989) 3518
8. Lacy P., Levi-Schaffer F., Mahmudi-Azer S., Bablitz B., Hagen S.C., Velazquez J., Kay A.B., and Moqbel R. Intracellular localization of interleukin-6 in eosinophils from atopic asthmatics and effects of interferon gamma. Blood 91 (1998) 2508
9. Lacy P., Mahmudi-Azer S., Bablitz B., Hagen S.C., Velazquez J.R., Man S.F., and Moqbel R. Rapid mobilization of intracellularly stored RANTES in response to interferon-gamma in human eosinophils. Blood 94 (1999) 23
10. Lacy P., Logan M.R., Bablitz B., and Moqbel R. Fusion protein vesicle-associated membrane protein 2 is implicated in IFN-gamma-induced piecemeal degranulation in human eosinophils from atopic individuals. J. Allergy Clin. Immunol. 107 (2001) 671
11. Levi-Schaffer F., Lacy P., Severs N.J., Newman T.M., North J., Gomperts B., Kay A.B., and Moqbel R. Association of granulocyte-macrophage colony-stimulating factor with the crystalloid granules of human eosinophils. Blood 85 (1995) 2579
12. Levi-Schaffer F., Barkans J., Newman T.M., Ying S., Wakelin M., Hohenstein R., Barak V., Lacy P., Kay A.B., and Moqbel R. Identification of interleukin-2 in human peripheral blood eosinophils. Immunology 87 (1996) 155
13. Lewis D.M., Lewis J.C., Loegering D.A., and Gleich G.J. Localization of the guinea pig eosinophil major basic protein to the core of the granule. J. Cell Biol. 77 (1978) 702
14. Li X., and Donowitz M. Fractionation of subcellular membrane vesicles of epithelial and nonepithelial cells by OptiPrep density gradient ultracentrifugation. Methods Mol. Biol. 440 (2008) 97
15. Logan M.R., Lacy P., Bablitz B., and Moqbel R. Expression of eosinophil target SNAREs as potential cognate receptors for vesicle-associated membrane protein-2 in exocytosis. J. Allergy Clin. Immunol. 109 (2002) 299
16. Logan M.R., Lacy P., Odemuyiwa S.O., Steward M., Davoine F., Kita H., and Moqbel R. A critical role for vesicle-associated membrane protein-7 in exocytosis from human eosinophils and neutrophils. Allergy 61 (2006) 777
17. Mahmudi-Azer S., Downey G.P., and Moqbel R. Translocation of the tetraspanin CD63 in association with human eosinophil mediator release. Blood 99 (2002) 4039
18. Melo R.C., Spencer L.A., Perez S.A., Ghiran I., Dvorak A.M., and Weller P.F. Human eosinophils secrete preformed, granule-stored interleukin-4 through distinct vesicular compartments. Traffic 6 (2005) 1047
19. Moqbel R., Ying S., Barkans J., Newman T.M., Kimmitt P., Wakelin M., Taborda-Barata L., Meng Q., Corrigan C.J., Durham S.R., and Kay A.B. Identification of messenger RNA for IL-4 in human eosinophils with granule localization and release of the translated product. J. Immunol. 155 (1995) 4939
20. Neves J.S., Perez S.A., Spencer L.A., Melo R.C., Reynolds L., Ghiran I., Mahmudi-Azer S., Odemuyiwa S.O., Dvorak A.M., Moqbel R., and Weller P.F. Eosinophil granules function extracellularly as receptor-mediated secretory organelles. Proc. Natl. Acad. Sci. U. S. A. 105 (2008) 18478
21. Persson T., Calafat J., Janssen H., Karawajczyk M., Carlsson S.R., and Egesten A. Specific granules of human eosinophils have lysosomal characteristics: presence of lysosome-associated membrane proteins and acidification upon cellular activation. Biochem. Biophys. Res. Commun. 291 (2002) 844
22. Rothenberg M.E., and Hogan S.P. The eosinophil. Annu. Rev. Immunol. 24 (2006) 147
23. Spencer L.A., Melo R.C., Perez S.A., Bafford S.P., Dvorak A.M., and Weller P.F. Cytokine receptor-mediated trafficking of preformed IL-4 in eosinophils identifies an innate immune mechanism of cytokine secretion. Proc. Natl. Acad. Sci. U. S. A. 103 (2006) 3333
24. Spencer L.A., Szela C.T., Perez S.A., Kirchhoffer C.L., Neves J.S., Radke A.L., and Weller P.F. Human eosinophils constitutively express multiple Th1, Th2, and immunoregulatory cytokines that are secreted rapidly and differentially. J. Leukoc. Biol. 85 (2008) 117
25. Stankiewicz M., Jonas W., Hadas E., Cabaj W., and Douch P.G. Supravital staining of eosinophils. Int. J. Parasitol. 26 (1996) 445
26. West B.C., Gelb N.A., and Rosenthal A.S. Isolation and partial characterization of human eosinophil granules. Comparison to neutrophils. Am. J. Pathol. 81 (1975) 575
27. Woerly G., Lacy P., Younes A.B., Roger N., Loiseau S., Moqbel R., and Capron M. Human eosinophils express and release IL-13 following CD28-dependent activation. J. Leukoc. Biol. 72 (2002) 769