On-line estimation of viable cells in a hybridoma culture at various DO levels using ATP balancing and redox potential measurement

Biotechnology and Bioengineering

Volumn 49, Issue 3, 1996, Pages 277-283

  Eyer, Kurt ^a,b   Heinzle, Elmar ^a  

^a ETH ZURICH   (Switzerland)

^b NESTLÉ RESEARCH CENTER   (Switzerland)

Author keywords

ATP balance; cell culture; dissolved oxygen; hybridoma; on line viable cell concentration; redox potential

Indexed keywords

ADENOSINETRIPHOSPHATE; COENZYMES; CORRELATION METHODS; ESTIMATION; FLOW OF FLUIDS; GLUCOSE; OXIDATION; OXYGEN; PH; PHASE COMPOSITION; REDOX REACTIONS;

BUBBLE AERATION; DISSOLVED OXYGEN; GLUTAMINE; GLYCOLYSIS; HYBRIDOMA CULTURE; LACTATE; REDOX POTENTIAL MEASUREMENT; REDUCED DICOTINAMIDE ADENINE; SURFACE AERATION;

ANIMAL CELL CULTURE;

ADENOSINE TRIPHOSPHATE;

AERATION; ANIMAL CELL; ARTICLE; BIOMASS; CELL CULTURE; CELL DENSITY; CELL VIABILITY; CELLULAR DISTRIBUTION; FLOW CYTOMETRY; GLYCOLYSIS; HYBRIDOMA; MOUSE; NONHUMAN; OXIDATION REDUCTION REACTION; OXIDATIVE PHOSPHORYLATION; OXYGEN CONSUMPTION;

ANIMALIA;

EID: 0030569899     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0290(19960205)49:3<277::AID-BIT5>3.0.CO;2-H     Document Type: Article

References (19)

1. Davey, C. L., Kell, D. B., Kemp. R., Meredith, R. W. 1988. On the audio- and radio-frequency dielectric behavior of anchorage-dependent, mouse L929-derived LS fibroblasts. Bioelectrochem. Bioenerg. 20: 83-98.
2. Ever, K. 1993. Entwicklung von On-line Analysemethoden zur Prozesskontrolle bei tierischen Zellkulturen, Dissertation ETH no. 10412, Zurich, Switzerland.
3. Eyer, K., Oeggerli, A., Heinzle, E. 1995. On-line gas analysis in animal cell cultivation: II. Methods of oxygen uptake rate estimation and its application to controlled feeding of glutamine. Biotechnol. Bioeng. 45: 54-62.
4. Glacken, M. W., Fleischaker, R. J., Sinskey, A. J. 1986. Reduction of waste product excretion via nutrient control: Possible strategies for maximizing product and cell yields on serum in cultures of mammalian cells. Biotechnol. Bioeng. 23: 1376-1389.
5. Hwang, C., Sinskey, A. J. 1991. The role of oxidation-reduction potential in monitoring growth of cultured mammalian cells, pp. 548-568. In: R. E. Spier, J. B. Griffiths, and B. Meigner (eds.). Production of biologicals from animal cells in culture. Butterworth-Heinemann, Oxford.
6. Kilburn, D. G., Fitzgerald, P., Blake-Colemann, B., Clarke, D., Griffith, B. 1989. On-line monitoring of cell mass in mammalian cell cultures by acoustic densitometry. Biotechnol. Bioeng. 33: 1379-1384.
7. Konstantinov, K. B., Pambayun, R. Matanguihan, R., Yoshida, T. Perusich, C. M., Hu, W.-S. 1992. On-line monitoring of hybridoma cell growth using a laser turbidity sensor. Biotechnol. Bioeng. 40: 1337-1342.
8. Leist, C., Meyer, H.-P., Fiechter, A. 1986. Process control during the suspension culture of a human melanoma cell line in a mechanically stirred loop reactor. J. Biotechnol. 4: 235-246.
9. MacMichael, G., Armiger, W. B., Lee, J. F., Mutharasan, R. 1987. On-line measurement of hybridoma growth by culture fluorescence. Biotechnol. Techn. 1: 213-218.
10. Merten, O. W., Palfi, G. E., Steiner, J. 1987. Invasive infrared sensors for the determination of the cell number in a continuous fermentation of hybridomas. Dev. Biol. Stand. 66: 357-360.
11. Oeggerli, A. 1992. On-line Überwachung von biologischen Prozessen mittels Quadrupol-Massenspektrometrie. Dissertation ETH no. 9816, Zurich, Switzerland.
12. Oeggerli, A., Eyer, K., Heinzle, E. 1995. On-line gas analysis in animal cell cultivation: I. Control of dissolved oxygen and pH. Biotechnol. Bioeng. 45: 42-53.
13. Peters, J. H., Baumgarten, H. 1990. Monoklonale Antikörper: Herstellung und Charakterisierung. Springer Verlag, Berlin.
14. Ramirez, O. T., Mutharasan, R. 1990. Cell cycle- and growthphase-dependent variations in size distribution, antibody productivity, and oxygen demand in hybridoma cultures. Biotechnol. Bioeng. 36: 839-848.
15. Roels, J. A. 1983. Energetics and kinetics in biotechnology. Elsevier, Amsterdam.
16. Schoen, R. C., Bentley, K. L., Klebe, R. J. 1982. Monoclonal antibody against human fibronectin which inhibits cell attachments. Hybridoma 1: 99-108.
17. Sen, S., Srienc, F., Hu, W.-S. 1989. Distinct volume distribution of viable and non-viable cells: a flow cytometric study. Cytotechnology 2: 85-94.
18. Zeng, A.-P., Ross, A., Deckwer, W.-D. 1990. A method to estimate the efficiency of oxidative phosphorylation and biomass yield from ATP of a facultative anaerobe in continuous culture. Biotechnol. Bioeng. 36: 965-969.
19. Zhou, W., Hu, W.-S. 1994. On-line characterization of a hybridoma cell culture process. Biotechnol. Bioeng. 44: 170-177.