Comparative proteome analysis using amine-reactive isobaric tagging reagents coupled with 2D LC/MS/MS in 3T3-L1 adipocytes following hypoxia or normoxia

Biochemical and Biophysical Research Communications

Volumn 383, Issue 1, 2009, Pages 135-140

  Choi, Sunkyu ^a,c   Cho, Kun ^a   Kim, Jaeyoon ^b   Yea, Kyungmoo ^b   Park, Gunwook ^a   Lee, Jeonghwa ^a   Ryu, Sung Ho ^b   Kim, Jeongkwon ^c   Kim, Young Hwan ^a  

^a Korea Basic Science Institute (KBSI)   (South Korea)

^b Pohang University of Science and Technology (POSTECH)   (South Korea)

^c Chungnam National University   (South Korea)

Author keywords

Adipocytes; Hypoxia; iTRAQ; Multi dimensional separation

Indexed keywords

AMINE; ANTIOXIDANT; CELL PROTEIN; ELONGATION FACTOR; GLYCOLYTIC ENZYME; PEPTIDE; PROTEOME;

ADIPOCYTE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL LYSATE; CELL STRAIN 3T3; COMPARATIVE STUDY; CONTROLLED STUDY; HYPOXIA; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METABOLISM; MOUSE; NONHUMAN; PRIORITY JOURNAL; PROTEOMICS; REAL TIME POLYMERASE CHAIN REACTION; STRESS;

3T3-L1 CELLS; ADIPOCYTES; ANIMALS; CELL HYPOXIA; CHROMATOGRAPHY, LIQUID; GLYCOLYSIS; MICE; OXIDATIVE STRESS; OXYGEN; PROTEIN BIOSYNTHESIS; PROTEOME; PROTEOMICS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; TANDEM MASS SPECTROMETRY;

EID: 64949193660     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2009.03.124     Document Type: Article

References (39)

1. Hosogai N., Fukuhara A., Oshima K., Miyata Y., Tanaka S., Segawa K., Furukawa S., Tochino Y., Komuro R., Matsuda M., and Shimomura I. Adipose tissue hypoxia in obesity and its impact on adipocytokine dysregulation. Diabetes 56 (2007) 901-911
2. Pallavicini M.G., Lalande M.E., Miller R.G., and Hill R.P. Cell cycle distribution of chronically hypoxic cells and determination of the clonogenic potential of cells accumulated in G2 + M phases after irradiation of a solid tumor in vivo. Cancer Res. 39 (1979) 1891-1897
3. Amellem O., and Pettersen E.O. Cell inactivation and cell cycle inhibition as induced by extreme hypoxia: the possible role of cell cycle arrest as a protection against hypoxia-induced lethal damage. Cell Prolif. 24 (1991) 127-141
4. Bunn H.F., and Poyton R.O. Oxygen sensing and molecular adaptation to hypoxia. Physiol. Rev. 76 (1996) 839-885
5. Semenza G.L. HIF-1, O(2), and the 3 PHDs: how animal cells signal hypoxia to the nucleus. Cell 107 (2001) 1-3
6. Bruick R.K. Oxygen sensing in the hypoxic response pathway: regulation of the hypoxia-inducible transcription factor. Genes Dev. 17 (2003) 2614-2623
7. Segawa K., Fukuhara A., Hosogai N., Morita K., Okuno Y., Tanaka M., Nakagawa Y., Kihara S., Funahashi T., Komuro R., Matsuda M., and Shimomura I. Visfatin in adipocytes is upregulated by hypoxia through HIF1alpha-dependent mechanism. Biochem. Biophys. Res. Commun. 349 (2006) 875-882
8. Hauner H. Secretory factors from human adipose tissue and their functional role. Proc. Nutr. Soc. 64 (2005) 163-169
9. Rosen E.D., and Spiegelman B.M. Adipocytes as regulators of energy balance and glucose homeostasis. Nature 444 (2006) 847-853
10. Brook C.G., Lloyd J.K., and Wolf O.H. Relation between age of onset of obesity and size and number of adipose cells. Br. Med. J. 2 (1972) 25-27
11. Wang B., Wood I.S., and Trayhurn P. Dysregulation of the expression and secretion of inflammation-related adipokines by hypoxia in human adipocytes. Pflugers Arch. 455 (2007) 479-492
12. Wood I.S., Wang B., Lorente-Cebrian S., and Trayhurn P. Hypoxia increases expression of selective facilitative glucose transporters (GLUT) and 2-deoxy-d-glucose uptake in human adipocytes. Biochem. Biophys. Res. Commun. 361 (2007) 468-473
13. Al-Shahrour F., Diaz-Uriarte R., and Dopazo J. FatiGO: a web tool for finding significant associations of gene ontology terms with groups of genes. Bioinformatics 20 (2004) 578-580
14. Wolters D.A., Washburn M.P., and Yates III J.R. An automated multidimensional protein identification technology for shotgun proteomics. Anal. Chem. 73 (2001) 5683-5690
15. Link A.J., Eng J., Schieltz D.M., Carmack E., Mize G.J., Morris D.R., Garvik B.M., and Yates III J.R. Direct analysis of protein complexes using mass spectrometry. Nat. Biotechnol. 17 (1999) 676-682
16. Cabiscol E., Belli G., Tamarit J., Echave P., Herrero E., and Ros J. Mitochondrial Hsp60, resistance to oxidative stress, and the labile iron pool are closely connected in Saccharomyces cerevisiae. J. Biol. Chem. 277 (2002) 44531-44538
17. Calandra T., Bernhagen J., Mitchell R.A., and Bucala R. The macrophage is an important and previously unrecognized source of macrophage migration inhibitory factor. J. Exp. Med. 179 (1994) 1895-1902
18. Thiele M., and Bernhagen J. Link between macrophage migration inhibitory factor and cellular redox regulation. Antioxid. Redox Signal. 7 (2005) 1234-1248
19. Beinert H., and Kennedy M.C. 19th Sir Hans Krebs lecture. Engineering of protein bound iron-sulfur clusters. A tool for the study of protein and cluster chemistry and mechanism of iron-sulfur enzymes. Eur. J. Biochem. 186 (1989) 5-15
20. Patel M., and Li Q.Y. Age dependence of seizure-induced oxidative stress. Neuroscience 118 (2003) 431-437
21. Powell C.S., and Jackson R.M. Mitochondrial complex I, aconitase, and succinate dehydrogenase during hypoxia-reoxygenation: modulation of enzyme activities by MnSOD. Am. J. Physiol. Lung Cell. Mol. Physiol. 285 (2003) L189-L198
22. Neumann C.A., and Fang Q. Are peroxiredoxins tumor suppressors?. Curr. Opin. Pharmacol. 7 (2007) 375-380
23. Fujii J., and Ikeda Y. Advances in our understanding of peroxiredoxin, a multifunctional, mammalian redox protein. Redox Rep. 7 (2002) 123-130
24. Kim H.J., Chae H.Z., Kim Y.J., Kim Y.H., Hwangs T.S., Park E.M., and Park Y.M. Preferential elevation of Prx I and Trx expression in lung cancer cells following hypoxia and in human lung cancer tissues. Cell Biol. Toxicol. 19 (2003) 285-298
25. Ho Y.S., and Crapo J.D. Isolation and characterization of complementary DNAs encoding human manganese-containing superoxide dismutase. FEBS Lett. 229 (1988) 256-260
26. White P., Doctor R.B., Dahl R.H., and Chen J. Coincident microvillar actin bundle disruption and perinuclear actin sequestration in anoxic proximal tubule. Am. J. Physiol. Renal Physiol. 278 (2000) F886-F893
27. Molitoris B.A., Leiser J., and Wagner M.C. Role of the actin cytoskeleton in ischemia-induced cell injury and repair. Pediatr. Nephrol. 11 (1997) 761-767
28. Duranteau J., Chandel N.S., Kulisz A., Shao Z., and Schumacker P.T. Intracellular signaling by reactive oxygen species during hypoxia in cardiomyocytes. J. Biol. Chem. 273 (1998) 11619-11624
29. Hohler B., Lange B., Holzapfel B., Goldenberg A., Hanze J., Sell A., Testan H., Moller W., and Kummer W. Hypoxic upregulation of tyrosine hydroxylase gene expression is paralleled, but not induced, by increased generation of reactive oxygen species in PC12 cells. FEBS Lett. 457 (1999) 53-56
30. Hogg T., Mesters J.R., and Hilgenfeld R. Inhibitory mechanisms of antibiotics targeting elongation factor Tu. Curr. Protein Pept. Sci. 3 (2002) 121-131
31. Yang Y.F., Chou M.Y., Fan C.Y., Chen S.F., Lyu P.C., Liu C.C., and Tseng T.L. The possible interaction of CDA14 and protein elongation factor 1alpha. Biochim. Biophys. Acta 1784 (2008) 312-318
32. McLeod L.E., and Proud C.G. ATP depletion increases phosphorylation of elongation factor eEF2 in adult cardiomyocytes independently of inhibition of mTOR signalling. FEBS Lett. 531 (2002) 448-452
33. Connolly E., Braunstein S., Formenti S., and Schneider R.J. Hypoxia inhibits protein synthesis through a 4E-BP1 and elongation factor 2 kinase pathway controlled by mTOR and uncoupled in breast cancer cells. Mol. Cell. Biol. 26 (2006) 3955-3965
34. Liu L., Cash T.P., Jones R.G., Keith B., Thompson C.B., and Simon M.C. Hypoxia-induced energy stress regulates mRNA translation and cell growth. Mol. Cell 21 (2006) 521-531
35. Heacock C.S., and Sutherland R.M. Induction characteristics of oxygen regulated proteins. Int. J. Radiat. Oncol. Biol. Phys. 12 (1986) 1287-1290
36. Kanski J., Behring A., Pelling J., and Schoneich C. Proteomic identification of 3-nitrotyrosine-containing rat cardiac proteins: effects of biological aging. Am. J. Physiol. Heart Circ. Physiol. 288 (2005) H371-H381
37. Hoang V.M., Foulk R., Clauser K., Burlingame A., Gibson B.W., and Fisher S.J. Functional proteomics: examining the effects of hypoxia on the cytotrophoblast protein repertoire. Biochemistry 40 (2001) 4077-4086
38. Schofield C.J., and Ratcliffe P.J. Oxygen sensing by HIF hydroxylases. Nat. Rev. Mol. Cell Biol. 5 (2004) 343-354
39. Semenza G.L. Targeting HIF-1 for cancer therapy. Nat. Rev. Cancer 3 (2003) 721-732