Gene cloning, expression, and characterization of a new carboxylesterase from Serratia sp. SES-01: Comparison with Escherichia coli BioHe enzyme

Journal of Microbiology and Biotechnology

Volumn 19, Issue 2, 2009, Pages 147-154

  Kwon, Min A ^a   Kim, Hyun Suk ^a   Oh, Joon Young ^b   Song, Bong Keun ^a   Song, Jae Kwang ^a  

^a Korea Research Institute of Chemical Technology   (South Korea)

^b Taegwang Chemicals Co Ltd   (South Korea)

Author keywords

Biocatalyst; BioH; Biotin biosynthesis; Carboxylesterase; Serratia sp.

Indexed keywords

2 PROPANOL; ACETONE; ACETONITRILE; ALCOHOL; BUTANOL; CARBOXYLESTERASE; DIMETHYL SULFOXIDE; GENOMIC DNA; METHANOL; ORGANIC SOLVENT; THIOL ESTER HYDROLASE; TRIBUTYRIN;

ALKALINITY; AMINO ACID SEQUENCE; ARTICLE; BACTERIAL GENETICS; BACTERIUM CULTURE; BACTERIUM ISOLATION; CONTROLLED STUDY; DNA LIBRARY; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ANALYSIS; LOW TEMPERATURE; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; POLYMERASE CHAIN REACTION; PROTEIN FAMILY; SEQUENCE HOMOLOGY; SERRATIA; SERRATIA PROTEAMACULANS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CARBOXYLESTERASE; CLONING, MOLECULAR; DNA, BACTERIAL; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENE LIBRARY; MOLECULAR SEQUENCE DATA; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA; SERRATIA; SUBSTRATE SPECIFICITY;

ESCHERICHIA COLI; SERRATIA PROTEAMACULANS; SERRATIA SP.;

EID: 64549138978     PISSN: 10177825     EISSN: 17388872     Source Type: Journal    
DOI: 10.4014/jmb.0804.287     Document Type: Article

References (16)

1. Akatsuka, H., E. Kawai, N. Sakurai, and K. Omori. 2003. The Serratia marcescens bioH gene encodes an esterase. Gene 302: 185-192.
2. Bornscheuer, U. T. 2002. Microbial carboxyl esterases: Classification, properties and application in biocatalysis. FEMS Microbiol. Rev. 26: 73-81.
3. Bower, S., J. B. Perkins, R. R. Yocum, C. L. Howitt, P. Rahaim, and J. Pero. 1996. Cloning, sequencing, and characterization of the Bacillus subtilis biotin biosynthetic operon. J. Bacteriol. 178: 4122-4130.
4. Eom, G. T., J. S. Rhee, and J. K. Song. 2006. An efficient secretion system for type I secretion pathway-dependent lipase, TliA, in Escherichia coli: Effect of relative expression levels and expression timing of passenger protein and ABC transporter. J. Microbiol. Biotechnol. 16: 1422-1428.
5. Ifuku, O., H. Miyaoka, N. Koga, J. Kishimoto, S. Haze, Y. Wachi, and M. Kajiwara. 1994. Origin of carbon atoms of biotin. 13CNMR studies on biotin biosynthesis in Escherichia coli. Eur. J. Biochem. 220: 585-591.
6. Lemoine, Y., A. Wach, and J. M. Jeltsch. 1996. To be free or not: The fate of pimelate in Bacillus sphaericus and in Escherichia coli. Mol. Microbiol. 19: 645-647.
7. Ploux, O., P. Soularue, A. Marquet, R. Gloeckler, and Y. Lemoine. 1992. Investigations of the first step of biotin biosynthesis in Bacillus sphaericus: Purification and characterization of the pimeloyl-CoA synthetase, and uptake of pimelate. Biochem. J. 287: 685-690.
8. Rodionov, D. A., A. A. Mironov, and M. S. Gelfand. 2002. Conservation of the biotin regulon and the BirA regulatory signal in Eubacteria and Archaea. Genome Res. 12: 1507-1516.
9. Sakurai, N., Y. Imai, and S. Komatsubara. 1995. Instability of the mutated biotin operon plasmid in a biotin-producing mutant of Serratia marcescens. J. Biotechnol. 43: 11-19.
10. Sakurai, N., Y. Imai, M. Masuda, S. Komatsubara, and T. Tosa. 1994. Improvement of a d-biotin-hyperproducing recombinant strain of Serratia marcescens. J. Biotechnol. 36: 63-73.
11. Sanishvili, R., A. F. Yakunin, R. A. Laskowski, T. Skarina, E. Evdokimova, A. Doherty-Kirby, et al. 2003. Integrating structure, bioinformatics, and enzymology to discover function: BioH, a new carboxylesterase from Escherichia coli. J. Biol. Chem. 278: 26039-26045.
12. Spröer, C., U. Mendrock, J. Swiderski, E. Lang, and E. Stackebrandt. 1999. The phylogenetic position of Serratia, Buttiauxella and some other genera of the family Enterobacteriaceae. Int. J. Syst. Bacteriol. 49: 1433-1438.
13. Tomczyk, N. H., J. E. Nettleship, R. L. Baxter, H. J. Crichton, S. P. Webster, and D. J. Campopiano. 2002. Purification and characterisation of the BIOH protein from the biotin biosynthetic pathway. FEBS Lett. 513: 299-304.
14. Watanabe, H., T. Shiratori, H. Shoji, S. Miyatake, Y. Okazaki, K. Ikuta, T. Sato, and T. Saito. 1997. A novel acyl-CoA thioesterase enhances its enzymatic activity by direct binding with HIV Nef. Biochem. Biophys. Res. Commun. 238: 234-239.
15. Webb, M. E., A. Marquet, R. R. Mendel, F. Rébeillé, and A. G. Smith. 2007. Elucidating biosynthetic pathways for vitamins and cofactors. Nat. Prod. Rep. 24: 988-1008.
16. Xie, X., W. W. Wong, and Y. Tang. 2007. Improving simvastatin bioconversion in Escherichia coli by deletion of bioH. Metab. Eng. 9: 379-386.