Flow cytometric cell cycle analysis of muscle precursor cells cultured within 3D scaffolds in a perfusion bioreactor

Biotechnology Progress

Volumn 25, Issue 1, 2009, Pages 286-295

  Flaibani, Marina ^a   Luni, Camilla ^a   Sbalchiero, Elisa ^a   Elvassore, Nicola ^a  

^a UNIVERSITY OF PADOVA   (Italy)

Author keywords

3d scaffold; Cell cycle analysis; Flow cytometry; Live dead; Perfusion bioreactor; Skeletal muscle cells

Indexed keywords

3D SCAFFOLD; CELL CYCLE ANALYSIS; LIVE/DEAD; PERFUSION BIOREACTOR; SKELETAL MUSCLE CELLS;

BIOCONVERSION; BIOREACTORS; CELL MEMBRANES; CELL PROLIFERATION; COLLAGEN; FLOW CYTOMETRY; INDUSTRIAL CHEMICALS; MUSCLE; NUCLEIC ACIDS; POPULATION STATISTICS; QUALITY CONTROL; SCAFFOLDS; THREE DIMENSIONAL;

CELL CULTURE;

ANIMAL; ARTICLE; BIOREACTOR; CELL CYCLE; CELL LINE; CYTOLOGY; FLOW CYTOMETRY; METHODOLOGY; MOUSE; MUSCLE CELL; PHYSIOLOGY; SKELETAL MUSCLE; TISSUE ENGINEERING;

ANIMALS; BIOREACTORS; CELL CYCLE; CELL LINE; FLOW CYTOMETRY; MICE; MUSCLE CELLS; MUSCLE, SKELETAL; TISSUE ENGINEERING;

EID: 64549103047     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1002/btpr.40     Document Type: Article

References (25)

1. Khetani SR, Bhatia SN. Engineering tissues for in vitro applications. Curr Opin Biotechnol. 2006;17:524-531.
2. Freshney R. Culture of Animal Cells-A Manual of Basic Techniques, 4th ed. New York: Wiley-Liss; 2000.
3. Martin I, Wendt D, Heberer M. The role of bioreactors in tissue engineering. Trends Biotechnol. 2004;22:80-86.
4. Gomes ME, Bossano CM, Johnston CM, Reis RL, Mikos AG. In vitro localization of bone growth factors in constructs of biodegradable scaffolds seeded with marrow stromal cells and cultured in flow perfusion bioreactor. Tissue Eng. 2006;12:177-188.
5. Wang Y, Uemura T, Dong J, Kojima H, Tanaka J, Tateishi T. Application of perfusion culture system improves in vitro and in vivo osteogenesis of bone marrow-derived osteoblastic cells in porous ceramic materials. Tissue Eng. 2003;9:1205-1214.
6. Martin Y, Vermette P. Bioreactors for tissue mass culture: design, characterization, and recent advances. Biomaterials. 2005; 26:7481-7503.
7. Bilodeau K, Mantovani D. Bioreactors for tissue engineering: focus on mechanical constraints. A comparative review. Tissue Eng. 2006;12:2367-2383.
8. Bancroft GN, Sikavitsas VI, Mikos AG. Design of a flow perfusion bioreactor system for bone tissue engineering applications. Tissue Eng. 2003;9:549-554.
9. Vunjak-Novakovic G, Radisic M, Obradovic B. Cardiac tissue engineering: effects of bioreactor flow environment on tissue constructs. J Chem Technol Biotechnol. 2006;81:485-190.
10. Radisic M, Cannizzaro C, Vunjak-Novakovic G. Scaffolds and fluid flow in cardiac tissue engineering. Fluid Dyn Mater Process. 2006;2:1-15.
11. Janssen FW, Oostra J, van Oorschot A, van Blitterswijk CA. A perfusion bioreactor system capable of producing clinically relevant volumes of tissue-engineered bone: in vivo bone formation showing proof of concept. Biomaterials. 2006;27:315-323.
12. Ormerod M. Flow Cytometry-A Practical Approach. Oxford University Press; 2000.
13. Alam S, Sen A, Behie LA, Kallos MS. Cell cycle kinetics of expanding populations of neural stem and progenitor cells in vitro. Biotechnol Bioeng. 2004;88:332-347.
14. Dang SM, Gerecht-Nir S, Chen J, Itskovitz-Eldor J, Zandstra PW. Controlled, scalable embryonic stem cell differentiation culture. Stem Cells. 2004;22:275-282.
15. Braccini A, Wendt D, Jaquiery C, Jakob M, Heberer M, Kenins L, Wodnar-Filipowicz A, Quarto R, Martin I. Three-dimensional perfusion culture of human bone marrow cells and generation of osteoinductive grafts. Stem Cells. 2005;23:1066-1072.
16. Schmidt D, Asmis LM, Odermatt B, Kelm J, Breymann C, Gossi M, Genoni M, Zund G, Hoerstrup SP. Engineered living blood vessels: functional endothelia generated from human umbilical cord-derived progenitors. Ann Thorac Surg. 2006;82: 1465-1471.
17. Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P. Molecular Biology of the Cell, 4th ed. New York: Garland; 2002. 18. Cannizzaro C, Tandon N, Figallo E, Park H, Gerecht S, Radisic M, Elvassore N, Vunjak-Novakovic G.Practical aspects of cardiac tissue engineering with electrical stimulation. Methods Mol Med, 2007;140:291-307.
18. Cimetta E, Flaibani M, Mella M, Serena E, Boldrin L, Coppi PD, Elvassore N. Enhancement of viability of muscle precursor cells on 3D scaffold in a perfusion bioreactor. Int J Artif Organs. 2007;30:415-128.
19. Wendt D, Jakob M, Martin I. Bioreactor-based engineering of osteochondral grafts: from model systems to tissue manufacturing. J Biosci Bioeng. 2005;100:489-194.
20. Strehl R, Tallheden T, Sjogren-Jansson E, Minuth WW, Lindahl A. Long-term maintenance of human articular cartilage in culture for biomaterial testing. Biomaterials. 2005;26:4540-4549.
21. Wendt D, Stroebel S, Jakob M, John GT, Martin I. Uniform tissues engineered by seeding and culturing cells in 3D scaffolds under perfusion at defined oxygen tensions. Biorheology. 2006;43(3/4):481-488.
22. Zhao F, Ma T. Perfusion bioreactor system for human mesenchymal stem cell tissue engineering: dynamic cell seeding and construct development. Biotechnol Bioeng. 2005;91:482-493.
23. Robinson J, Darzynkiewicz Z, Dean P, Dressler L, Rabinovich P, Stewart C, Tanke H, Wheeless L. Current Protocols in Cytometry. New York: Wiley; 1997.
24. Anderson HJ, de Jong G, Vincent I, Roderge M. Flow cytometry of mitotic cells. Exp Cell Res. 1998;238:498-502.
25. Figallo E, Flaibani M, Zavan B, Abatangelo G, Elvassore N. Micropatterned biopolymer 3D scaffold for static and dynamic culture of human fibroblasts. Biotechnol Prog. 2007;23:210-216.