Quantitation of soluble aggregates in recombinant monoclonal antibody cell culture by pH-gradient protein A chromatography

Analytical Biochemistry

Volumn 388, Issue 2, 2009, Pages 273-278

  Pan, Hai ^a   Chen, Ken ^a   Pulisic, Matt ^a   Apostol, Izydor ^a   Huang, Gang ^a  

^a Analytical and Formulation Sciences   (United States)

Author keywords

Aggregation; Cell culture; Monoclonal antibody; pH gradient protein A chromatography; Size exclusion chromatography

Indexed keywords

AGGLOMERATION; AGGREGATES; ANIMAL CELL CULTURE; CELL CULTURE; CELLS; MAMMALS; MONOCLONAL ANTIBODIES; PH; RECOMBINANT PROTEINS; REMOVAL; SIZE SEPARATION;

HIGHER-ORDER; HUMAN IMMUNOGLOBULIN; LABOR INTENSIVE; MAMMALIAN CELL CULTURE; MONOCLONAL ANTIBODIES (MABS); PROTEIN A CHROMATOGRAPHY; RECOMBINANT MONOCLONAL ANTIBODY; SOLUBLE AGGREGATES;

SIZE EXCLUSION CHROMATOGRAPHY;

IMMUNOGLOBULIN G1 ANTIBODY; IMMUNOGLOBULIN G2; MONOCLONAL ANTIBODY; PROTEIN A; RECOMBINANT ANTIBODY;

ANIMAL CELL; ARTICLE; CELL CULTURE; CELL PH; CELL SEPARATION; CHROMATOGRAPHY; CONTROLLED STUDY; FEMALE; GEL PERMEATION CHROMATOGRAPHY; HUMAN; MAMMAL CELL; NONHUMAN; PH GRADIENT PROTEIN A CHROMATOGRAPHY; PRIORITY JOURNAL; PURIFICATION;

MAMMALIA;

EID: 64249156466     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2009.02.037     Document Type: Article

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