Molecular design of a nylon-6 byproduct-degrading enzyme from a carboxylesterase with a β-lactamase fold

FEBS Journal

Volumn 276, Issue 9, 2009, Pages 2547-2556

  Kawashima, Yasuyuki ^a   Ohki, Taku ^a   Shibata, Naoki ^a,b   Higuchi, Yoshiki ^a,b   Wakitani, Yoshiaki ^a   Matsuura, Yusuke ^a   Nakata, Yusuke ^a   Takeo, Masahiro ^a   Kato, Dai Ichiro ^a   Negoro, Seiji ^a  

^a UNIVERSITY OF HYOGO   (Japan)

^b RIKEN HARIMA INSTITUTE   (Japan)

Author keywords

lactamase; 6 aminohexanoate dimer hydrolase; Carboxylesterase; Nylon oligomer; X ray crystallography

Indexed keywords

AMIDE; ASPARAGINE; ASPARTIC ACID; BETA LACTAMASE; CARBOXYLESTERASE; HISTIDINE; MUTANT PROTEIN; NYLON; TYROSINE;

AMINO TERMINAL SEQUENCE; ARTHROBACTER; ARTICLE; BINDING AFFINITY; BINDING SITE; CARBOXY TERMINAL SEQUENCE; COMPLEX FORMATION; ENZYME KINETICS; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; HYDROGEN BOND; HYDROLYSIS; HYDROPHOBICITY; MOLECULAR INTERACTION; MOLECULAR STABILITY; MUTATION; NONHUMAN; PRIORITY JOURNAL; PROTEIN FOLDING; SITE DIRECTED MUTAGENESIS; WILD TYPE; X RAY CRYSTALLOGRAPHY;

AMINO ACID SUBSTITUTION; ARTHROBACTER; BETA-LACTAMASES; CAPROLACTAM; CARBOXYLESTERASE; CATALYTIC DOMAIN; HYDROGEN BONDING; HYDROLYSIS; KINETICS; MODELS, MOLECULAR; POLYMERS; PROTEIN FOLDING; STRUCTURE-ACTIVITY RELATIONSHIP;

ARTHROBACTER;

EID: 64149121651     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2009.06978.x     Document Type: Article

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