Discovery of a novel carboxylesterase through functional screening of a pre-enriched environmental library

Journal of Applied Microbiology

Volumn 106, Issue 5, 2009, Pages 1532-1539

  Rashamuse, K ^b   Ronneburg, T ^b   Hennessy, F ^b   Visser, D ^b   Van Heerden, E ^c   Piater, L ^c   Litthauer, D ^c   Moller C ^c   Brady, D ^a,b  

^a CSIR Biosciences   (South Africa)

^b CSIR Biosciences   (South Africa)

^c UNIVERSITY OF THE FREE STATE   (South Africa)

Author keywords

Carboxylesterase; Environmental library; Functional screening

Indexed keywords

ENVIRONMENTAL TECHNOLOGY; ENZYMES; ESCHERICHIA COLI; MINING; SCREENING; SUBSTRATES;

CARBOXYLESTERASES; ENVIRONMENTAL LIBRARY; ENVIRONMENTAL SAMPLE; FUNCTIONAL SCREENING; GENE LIBRARIES; HETEROLOGOUS EXPRESSION; NUCLEOTIDE SEQUENCE ANALYSIS; PRE-ENRICHMENT; RECOMBINANT PLASMID; WALKING STRATEGY;

ESTERS;

AMINO ACID; CARBOXYLESTERASE; ESTAM PROTEIN; UNCLASSIFIED DRUG;

AMINO ACID; ENZYME; GENE EXPRESSION; GENOME; PHENOTYPE; SUBSTRATE;

ARTICLE; ENZYME ASSAY; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STRUCTURE; ESCHERICHIA COLI; GENE INSERTION; GENE LIBRARY; NUCLEOTIDE SEQUENCE; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN MOTIF; RNA TRANSLATION; SEQUENCE ANALYSIS;

ESCHERICHIA COLI;

EID: 64149101058     PISSN: 13645072     EISSN: 13652672     Source Type: Journal    
DOI: 10.1111/j.1365-2672.2008.04114.x     Document Type: Article

References (34)

1. Altschul, S.F., Madden, T.S., Schäffer, A.A., Zhang, J., Zhang, Z., Miller, W. Lipman, D.J. (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucl Acids Res 25, 3389 3402.
2. Appel, R.D., Bairoch, A. Hochstrasser, D.F. (1994) A new generation of information retrieval tools for biologists: the example of the EXPASY WWW server. Trends Biochem Sci 19, 258 260.
3. Arpigny, K.L. Jaeger, K.E. (1999) Bacterial lipolytic enzymes: classification and properties. J Biochem 343, 177 183.
4. Bendten, J.D., Nielsen, H., von Heijnie, G. Brunak, S. (2004) Improved prediction of signal peptide: SignalP 3.0. J Mol Biol 340, 783 795.
5. Bornscheuer, U.T. (2002) Microbial carboxylesterases: classification, properties and application in biocatalysis. FEMS Microbiol Rev 26, 73 81.
6. Botes, E., Van Heerden, E. Litthauer, D. (2007) Hyper-resistance to arsenic in bacteria isolated from antimony mine in South Africa. S Afr J Sci 103, 279 282.
7. Bradford, M.M. (1976) A rapid and sensitive method for quantification of microgram quantities of protein utilizing the principle of protein-dye binding. J Anal Biochem 72, 248 254.
8. Buchholz, K., Kasche, V. Bornscheuer, U.T. (2005) Biocatalysts and enzyme technology pp. 127 165. Weinheim : Wiley-VCH.
9. Choo, D.W., Kurihara, T., Suzuki, T., Soda, K. Esaki, N. (1998) A cold-adapted lipase from an Alaskan psychrotroph, Pseudomonas sp. strain B11-1: gene cloning and enzyme purification and characterization. J Appl Environ Microbiol 64, 486 491.
10. Cowan, D., Meyer, Q., Stafford, W., Muyanga, S., Cameron, R. Wittwer, P. (2005) Metagenomic gene discovery: past, present and future. Trends Biotech 23, 321 329.
11. Drauz, K. Waldmann, H. (1995) Enzyme Catalysis in Organic Synthesis, Vols 1 and 2, pp. 135 164. Weinheim : Wiley-VCH.
12. Gabor, E.M., Alkema, W.B. Janssen, D.B. (2004) Quantifying the accessibility of the metagenome by random expression cloning technique. J Appl Environ Microbiol 6, 948 958.
13. Hall, T.A. (1990) BioEdit: a user friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl acids Symp Ser 41, 95 98.
14. Henke, E., Pleiss, J. Bornscheuer, U.T. (2002) Activity of lipases and esterases towards tertiary alcohols: insights into structure-function relationships. Angew Chem Int Ed 41, 3211 3213.
15. Hotta, Y., Ezak, I.S., Atomi, H. Imanaka, T. (2002) Extremely stable and versatile carboxylesterase from a hyperthermophilic Archaeon. Am Soc Microbiol 68, 3925 3931.
16. Jaeger, K.E., Ransaic, S., Kock, B.H., Feratto, F.G. Dijkstra, B.W. (1993) Topology characterization and modeling of the 3D structure of lipase from Pseudomonas aeruginosa. FEBS Lett 332, 143 149.
17. Jaeger, K.E., Dijkstra, B.W. Reetz, M.T. (1999) Bacterial biocatalysis: molecular biology, three-dimensional structures, and biotechnological applications of lipases. Ann Rev Microbiol 53, 315 351.
18. Knietsch, A., Waschkowitz, T., Bowien, S., Henne, A. Daniel, R. (2003) Construction and screening of metagenomic libraries derived from enrichment cultures: generation of a gene bank for genes conferring alcohol oxidoreductase activity on Escherichia coli. J Appl Environ Microbiol 69, 1408 1416.
19. Laemmli, U.K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227, 680 685.
20. Largarde, D., Nguyen, H.K., Ravot, G., Wahler, D., Reymond, J.L., Hills, G., Veit, T. Lefevre, F. (2002) High-throughput screening of thermostable esterases for industrial bioconversions. Org Process Res Dev 6, 441 445.
21. Manco, G., Giosuè, E., D'Auria, S., Herman, P., Carrea, G. Rossi, M. (2000) Cloning, overexpression, and properties of a new thermophilic and thermostable esterase with sequence similarity to hormone-sensitive lipase subfamily from the archaeon Archaeoglobus fulgidus. Arch Biochem Biophys 373, 182 192.
22. Mergeay, M., Monchy, S., Vallaeys, T., Auquier, V., Benotmane, A., Bertin, P., Taghavi, S., Dunn, J. et al. (2003) Ralstonia metallidurans, a bacterium specifically adapted to toxic metals: towards a catalogue of metal-responsive genes. FEMS Microbiol Rev 27, 385 410.
23. Motulsky, H. Christopoulos, A. (2004) Fitting Models to Biological Data using Linear and Nonlinear Regression. A Practical Guide to Curve Fitting. New York : Oxford University Press.
24. Muller, D., Médigue, C., Koechler, S., Barbe, V., Barakat, M., Talla, E., Bonnefoy, V., Krin, E. et al. (2007) A tale of two oxidation states: bacterial colonization of arsenic-rich environments. PLoS Genet 3, e53.
25. Myrick, K.V. Gelbart, W.M. (2002) Universal fast walking for direct and versatile determination of flanking sequence. Gene 284, 125 131.
26. Rashamuse, K., Burton, S., Stafford, W. Cowan, D. (2007a) Molecular characterization of a novel family VIII esterase from Burkholderia multivorans UWC10. J Mol Microbiol Biotechnol 13, 181 188.
27. Rashamuse, K., Burton, S. Cowan, D. (2007b) A novel recombinant ethyl ferulate esterase from Burkholderia multivorans. J Appl Microbiol 103, 1610 1620.
28. Rhee, J.-K., Ahn, D.-G., Kim, Y.-G. Oh, J.-W. (2005) New thermophilic and thermostable esterase with sequence similarity to the hormone-sensitive lipase family, cloned from a metagenomic library. Appl Environ Microbiol 71, 817 825.
29. Roh, C. Villatte, F. (2008) Isolation of a low-temperature adapted lipolytic enzyme from uncultivated micro-organisms. J Appl Microbiol 105, 116 123.
30. von Rozycki, T., Nies, D.H. Saier, M.H. Jr. (2005) Genomic analyses of transport proteins in Ralstonia metallidurans. Comp Funct Genome 6, 17 56.
31. Sambrook, J. Russell, D.W. (2001) Molecular Cloning: A Laboratory Manual, 3rd edn. Cold Spring Harbor, NY : Cold Spring Harbor Laboratory Press.
32. Schmeisser, C., Steele, H. Streit, W.R. (2007) Metagenomics, biotechnology with non-culturable microbes. Appl Microbiol Biotechnol 75, 955 962.
33. Thompson, J.D., Gibson, T.J., Plewniak, F., Jeanmougin, F. Higgins, D.G. (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res 25, 4876 4882.
34. Venter, J., Smith, C.H. Hood, L. (1996) A new strategy for genome sequencing. Nature 381, 364 366.