Deletion of aprA and nprA genes for alkaline protease A and neutral protease A from Bacillus thuringiensis: Effect on insecticidal crystal proteins

Journal of Biotechnology

Volumn 84, Issue 1, 2000, Pages 67-72

  Tan, Yuping ^a   Donovan, William P ^a  

^a Ecogen Inc   (United States)

Author keywords

Bacillus thuringiensis; Insecticidal crystal protein; Protease

Indexed keywords

GENES; INSECTICIDES; PROTEINS;

ALKALINE PROTEASE; INSECTICIDAL CRYSTAL PROTEINS;

ENZYME KINETICS;

ALKALINE PROTEINASE; BACTERIAL ENZYME; EDETIC ACID; INSECTICIDE; NEUTRAL PROTEINASE; PROTEIN;

ARTICLE; BACILLUS THURINGIENSIS; ENZYME INHIBITION; GENE DELETION; LEPIDOPTERA; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN DETERMINATION; PROTEIN SYNTHESIS;

ALLELES; ANIMAL; BACILLUS THURINGIENSIS; BACTERIAL PROTEINS; BLOTTING, SOUTHERN; CHELATING AGENTS; CLONING, MOLECULAR; DNA, BACTERIAL; EDETIC ACID; ENDOTOXINS; ENZYME ACTIVATION; GENE DELETION; GENE EXPRESSION REGULATION, BACTERIAL; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENOTYPE; LARVA; LEPIDOPTERA; SERINE ENDOPEPTIDASES;

EID: 0034681010     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0168-1656(00)00328-X     Document Type: Article

References (31)

1. Andrews R.E. Jr, Bibilos M.M., Bulla L.A. Jr. Protease activation of the entomocidal protoxin of Bacillus thuringiensis subsp. kurstaki. Appl. Environ. Microbiol. 50:1985;737-742.
2. Carroll J., Li J., Ellar D.J. Proteolytic processing of a coleopteran-specific δ-endotoxin produced by Bacillus thuringiensis var. tenebrionis. Biochem. J. 261:1989;99-105.
3. Chestukhina G.G., Zalunin I.A., Kostina L.I., Kotova T.S., Kattrukha S.P., Stepanov V.M. Crystal-forming proteins of Bacillus thuringiensis. Biochem. J. 187:1980;457465.
4. Chestukhina G.G., Kostina L.I., Mikhailova A.L., Tyurin S.A., Klepikova F.S., Stepanov V.M. The main features of Bacillus thuringiensis 5-endotoxin molecular structure. Arch. Microbiol. 132:1982;159-162.
5. Dai S.-M., Gill S.S. In vitro and in vivo proteolysis of the Bacillus thuringiensis subsp. israelensis CryIVD protein by Culex quinquefasciatus larval midgut proteases. Insect Biochem. Mol. Biol. 23:1993;273-283.
6. Dalhammar G., Steiner H. Characterization of inhibitor A, a protease from Bacillus thuringiensis which degrades attacins and cecropins, two classes of antibacterial proteins in insects. Eur. J. Biochem. 139:1984;247-252.
7. Donovan W.P., Dankocsik C.C., Gilbert M.P., Gawron-Burke M.C., Groat R.G., Carlton B.C. Amino acid sequence and entomocidal activity of the P2 crystal protein. J. Biol. Chem. 263:1988;561-567.
8. Donovan, W.P., Rupar, M.J., Slaney, A.C., 1993. Bacillus thuringiensis cryIIIC(B) toxin gene and protein toxic to coleopteran insects. US Patent 5 264 364.
9. Donovan, W.P., Tan, Y., Jany, C.S., Gonzalez, J.M. Jr., 1994. Bacillus thuringiensis cryET4 and cryET5 toxin genes and proteins toxic to lepidopteran insects. US Patent 5 342 687.
10. Donovan W.P., Tan Y., Slaney A.C. Cloning of the nprA gene for neutral protease A of Bacillus thuringiensis and effect of in vivo deletion of nprA on insecticidal crystal protein. Appl. Environ. Microbiol. 63:1997;2311-2317.
11. Forcada C., Alcacer E., Garcera M.D., Martinez R. Differences in the midgut proteolytic activity of two Heliothis virescens strains, one susceptible and one resistant to Bacillus thuringiensis toxins. Arch. Insect Biochem. Physiol. 31:1996;257-272.
12. Haider M.Z., Knowles B.H., Ellar D.J. Specificity of Bacillus thuringiensis var. colmeri insecticidal 6-endotoxin is determined by differential proteolytic processing of the protoxin by larval gut proteases. Eur. J. Biochem. 156:1986;531-540.
13. Hofte H., Whiteley H.R. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol. Rev. 53:1989;242-255.
14. Keller M., Sneh B., Strizhov N., Prudovsky E., Regev A., Konca C., Schell J., Zilberstein A. Digestion of 5-endotoxin by gut proteases may explain reduced sensitivity of advanced instar larvae of Spodoptera littoralis to Cry1C. Insect Biochem. Mol. Biol. 26:1996;365-373.
15. Kumar N.S., Venkateswerlu G. Involvement of an endogenous metalloprotease in the activation of protoxin in Bacillus thuringiensis subsp. kurstaki. Biochem. Mol. Biol. Int. 42:1997;901-908.
16. Kumar N.S., Venkateswerlu G. Endogenous protease-activated 66-kDa toxin from Bacillus thuringiensis subsp. kurstaki active against Spodoptera littoralis. FEMS Microbiol. Lett. 159:1998;113-120.
17. Lovgren A., Zhang M., Engstrom A., Dalhammar G., Landen R. Molecular characterization of immune inhibitor A, a secreted virulence protease from Bacillus thuringiensis. Mol. Microbiol. 4:1990;2137-2146.
18. MacIntosh S.C., Kishore G.M., Perlak F.J., Marrone P.G., Stone T.B., Sims S.R., Fuchs R.L. Potentiation of Bacillus thuringiensis insecticidal activity by serine protease inhibitors. J. Agric. Food Chem. 38:1990;1145-1152.
19. Martinez-Ramirez A.C., Real M.D. Proteolytic processing of Bacillus thuringiensis CryIIIA toxin and specific binding to brush-border membrane vesicles of Leptinotarsa decemlineata (Colorado potato beetle). Pesticide Biochem. Physiol. 54:1996;115-122.
20. Moriyama R., Sugimoto K., Zhang H., Inoue T., Makino S. A cysteine-dependent serine protease associated with the dormant spores of Bacillus cereus: purification of the protein and cloning of the corresponding gene. Biosci. Biotechnol. Biochem. 62:1998;268-274.
21. Nickerson K.W., Swanson J.D. Removal of contaminating proteases from Bacillus thuringiensis parasporal crystals by density gradient centrifugation in NaBr. Eur. J. Appl. Microbiol. Biotechnol. 13:1981;213-215.
22. Ogiwara K., Indrasith L.S., Asano S., Hori H. Processing of 6-endotoxin from Bacillus thuringiensis subsp. kurstaki HD-1 and HD-73 by gut juices of various insect larvae. J. Invert. Pathol. 60:1992;121-126.
23. Oppert B., Kramer K.J., Johnson D., Upton S.J., McGaughey W.H. Luminal proteinases from Plodia interpunctella and the hydrolysis of Bacillus thuringiensis Cry1A(c) protoxin. Insect Biochem. Mol. Biol. 26:1996;571-583.
24. Oppert B., Kramer K.J., Beeman R.W., Johnson D., McGaughey W.H. Proteinase-mediated insect resistance to Bacillus thuringiensis toxins. J. Biol. Chem. 272:1997;23473-23476.
25. Perlman D., Halvorson H.O. A putative signal peptidase recognition site and sequence in eukaryotic and prokaryotic signal peptides. J. Mol. Biol. 167:1983;391-409.
26. Sarath G., de la Motte R.S., Wagner F.W. Protease assay methods. Beynon R.J., Bond J.S. Proteolytic Enzymes: A Practical Approach. 1989;25-54 IRL Press, Oxford.
27. Schnepf E., Crickmore N., Van Rie J., Lereclus D., Baum J., Feitelson J., Zeigler D.R., Dean D.H. Bacillus thuringiensis and its pesticidal crystal proteins. Microbiol. Mol. Biol. Rev. 62:1998;775-806.
28. Stepanov V.M., Chestukhina G.G., Rudenskaya G.N., Epremyan A.S., Osterman A.L., Khodova O.M., Belyanova L.P. A new subfamily of microbial serine proteinases? Structural similarities of Bacillus thuringiensis and Thermoactinomyces vulgaris extracellular serine proteinases. Biochem. Biophys. Res. Commun. 100:1981;1680-1687.
29. Reddy S.T., Kumar N.S., Venkateswerlu G. Identification and purification of the 69-kDa intracellular protease involved in the proteolytic processing of the crystal 6-endotoxin of Bacillus thuringiensis subsp. tenebrionis. FEMS Microbiol. Lett. 183:2000;63-66.
30. Tabashnik B.E., Finson N., Johnson M.W. Two protease inhibitors fail to synergize Bacillus thuringiensis in diamondback moth (Lepidoptera: Plutellidae). J. Econ. Entomol. 85:1992;2082-2087.
31. Youngman P., Poth H., Green B., York K., Olmedo G., Smith K. Methods for genetic manipulation, cloning, and functional analysis of sporulation genes in Bacillus subtilis. Smith I., Slepecky R.A., Setlow P. Regulation of Procaryotic Development. 1989;65-78 American Society for Microbiology, Washington, DC.