Synergistic activity of a Bacillus thuringiensis δ-endotoxin and a bacterial endochitinase against Spodoptera littoralis larvae

Applied and Environmental Microbiology

Volumn 62, Issue 10, 1996, Pages 3581-3586

  Regev, Avital ^a   Keller, Menachem ^a   Strizhov, Nicolai ^b   Sneh, Baruch ^a   Prudovsky, Evgenya ^a   Chet, Ilan ^c   Ginzberg, Idit ^a   Koncz Kalman, Zsuzsanna ^b   Koncz, Csaba ^b   Schell, Jeff ^b   Zilberstein, Aviah ^a  

^a TEL AVIV UNIVERSITY   (Israel)

^b HEBREW UNIVERSITY OF JERUSALEM   (Israel)

^c MAX PLANCK INSTITUTE FOR PLANT BREEDING RESEARCH   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

ENDOTOXIN; PROTEIN;

ARTICLE; BACILLUS THURINGIENSIS; BACTERIAL COLONIZATION; ENZYME ACTIVITY; ESCHERICHIA COLI; LARVA; LEPIDOPTERA; PLASMID; PROTEIN ANALYSIS; TRANSGENIC ANIMAL;

ANIMALIA; BACILLUS THURINGIENSIS; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; INSECTA; LEPIDOPTERA; SERRATIA MARCESCENS; SPODOPTERA LITTORALIS;

EID: 10144263280     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.62.10.3581-3586.1996     Document Type: Article

References (54)

1. Arantes, O., and D. Lereclus. 1991. Construction of cloning vectors for Bacillus thuringiensis. Gene 108:115-119.
2. Aronson, A. I. 1993. The two faces of Bacillus thuringiensis insecticidal proteins and post-exponential survival. Mol. Microbiol. 7:489-496.
3. Aronson, A. I., D. Wu, and C. Zhang. 1995. Mutagenesis of specificity and toxicity regions of a Bacillus thuringiensis protoxin gene. J. Bacteriol. 177: 4059-4065.
4. Brandt, C. R., M. J. Adang, and K. D. Spence. 1978. The peritrophic membrane: ultrastructural analysis and function as a mechanical barrier to microbial infection in Orgyia pseudotsugata. J. Invertebr. Pathol. 32:12-24.
5. Broza, M., B. Sneh, A. Yawetz, U. Oron, and A. Honigman. 1984. Commercial application of Bacillus thuringiensis var. entomocidus to cotton fields for the control of Spodoptera littoralis Boisduval (Lepidoptera, Noctuidae). J. Econ. Entomol. 77:1530-1533.
6. Chak, K.-F., M.-Y. Tseng, and T. Yamamoto. 1994. Expression of the crystal protein gene under the control of the α-amylase promoter in Bacillus thuringiensis strains. Appl. Environ. Microbiol. 60:2304-2310.
7. Chen, X. J., A. Curtiss, E. Alcantara, and D. H. Dean. 1995. Mutations in domain I of Bacillus thuringiensis δ-endotoxin CryIA(b) reduce the irreversible binding of toxin to Manduca sexta brush border membrane vesicles. J. Biol. Chem. 270:6412-6419.
8. Chet, I., A. Ordenlich, R. Shapira, and A. B. Oppenheim. 1990. Mechanisms of biocontrol of soilborne plant pathogens with rhizobacteria. Plant Soil 129:85-92.
9. Derksen, A. C. G., and R. R. Granados. 1988. Alteration of a lepidopteran peritrophic membrane by baculovirus and enhancement of viral infection. Virology 167:242-250.
10. Dow, J. A. T. 1986. Insect midgut function. Adv. Insect Physiol. 19:187-290.
11. Duncan, D. B. 1951. A significance test for differences between ranked treatments in an analysis of variance. Va. J. Sci. 2:171-189.
12. Ely, S. 1993. The engineering of plants to express Bacillus thuringiensis δ-endotoxins, p. 105-124. In P. F. Entwistle, J. S. Cory, M. J. Baily, and S. Higgs (ed.), Bacillus thuringiensis, an environmental biopesticide: theory and practice. John Wiley & Sons, New York.
13. Gill, S. S., A. Cowles, and P. V. Pietrantonio. 1992. The mode of action of Bacillus thuringiensis endotoxins. Annu. Rev. Entomol. 37:615-636.
14. Grochulski, P., L. Masson, S. Borisova, M. Pusztai-Carey, J.-L. Schwartz, R. Brousseau, and M. Cygler. 1995. Bacillus thuringiensis CryIA(a) insecticidal toxin: crystal structure and channel formation. J. Mol. Biol. 254:447-464.
15. Hamal, M., M. Broza, M. Brownbridge, and B. Sneh. 1991. Screening for highly effective isolates of against Spodoptera exempta and Spodoptera littoralis. Phytoparasitica 19:9-21.
16. Hofte, H., H. de Greve, J. Seurinck, S. Jansens, J. Mahillon, C. Ampe, J. Vandekerckhove, H. Van der Bruggen, M. Van Montague, M. Zabeau, and M. Vaeck. 1986. Structural and functional analysis of a cloned δ-endotoxin of Bacillus thuringiensis Berliner 1715. Eur. J. Biochem. 161:273-280.
17. Hofte, H., and H. R. Whiteley. 1989. Insecticidal crystal proteins of Bacillus thuringiensis. Microbiol. Rev. 53:242-255.
18. Ishaaya, I., I. Moore, and D. Joseph. 1971. Protease and amylase activity in larvae of the Egyptian cotton leafworm, Spodoptera littoralis. J. Insect Physiol. 17:945-953.
19. Keller, M., B. Sneh, N. Strizhov, E. Prudovski, M. Regev, C. Koncz, J. Schell, and A. Zilberstein. 1996. Digestion δ-endotoxin by midgut proteases may explain reduced sensitivity of advanced instars of Spodoptera littoralis to CryIC. Insect Biochem. Mol. Biol. 26:365-373.
20. Koziel, M. G., G. L. Beland, C. Bowman, N. B. Carozzi, R. Crenshaw, L. Crossland, J. Dawson, N. Desai, M. Hill, L. Kadwell, K. Launis, K. Lewis, D. Maddox, K. McPherson, M. R. Meghji, E. Merlin, R. Rhodes, G. W. Warren, M. Wright, and V. S. Evola. 1993. Field performance of elite transgenic maize plants expressing an insecticidal protein derived from Bacillus thuringiensis. Bio/Technology 11:194-200.
21. Li, J., J. Carrol, and D. L. Ellar. 1991. Crystal structure of insecticidal δ-endotoxin from Bacillus thuringiensis at 2.5A resolution. Nature (London) 353:815-821.
22. Lorence, A., A. Darszon, C. Diaz, A. Lievano, R. Quinotero, and A. Bravo. 1995. δ-Endotoxins induce cation channels in Spodoptera frugiperda brush border membranes in suspension and in planar lipid bilayers. FEBS Lett. 360:217-222.
23. Marder, J. B., A. K. Mattoo, and M. Edelman. 1986. Identification and characterization of the psbA gene product: the 32kDa chloroplast membrane protein. Methods Enzymol. 118:384-396.
24. Martens, W. M., B. Visser, J. M. Vlak, and D. Bosch. 1995. Mapping and characterization of the Bacillus thuringiensis CryIA(b) protoxin. Mol. Gen. Genet. 247:482-487.
25. McBride, K. E., Z. Svab, D. J. Schaaf, P. S. Hogan, D. M. Stalker, and P. Maliga. 1995. Amplification of a chimeric Bacillus thuringiensis gene in chloroplast leads to an extraordinary level of insecticidal protein in tobacco. Bio/Technology 13:362-365.
26. McGaughey, W. H., and D. E. Johnson. 1992. Indian meal moth resistance to different strains and mixtures of Bacillus thuringiensis. J. Econ. Entomol. 85:1594-1600.
27. Murray, E. E., T. Rocheleau, M. Eberle, C. Stock, V. Sekar, and M. Adang. 1991. Analysis of unstable RNA transcripts of insecticidal crystal protein genes of Bacillus thuringiensis in transgenic plants and electroporated protoplasts. Plant Mol. Biol. 16:1035-1050.
28. Oberkovicz, M. G., F. J. Perlak, K. Kusano-Krezmer, E. J. Mayer, and L. S. Walrud. 1986. Integration of the δ-endotoxin gene of Bacillus thuringiensis into the chromosome of root-colonizing strains of pseudomonads using Tn5. Gene 45:327-331.
29. Perlak, F. J., R. L. Fuchs, D. A. Dean, S. A. McPherson, and D. A. Fischoff. 1991. Modification of the coding sequence enhances plant expression of insect control protein genes. Proc. Natl. Acad. Sci. USA 88:3324-3328.
30. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
31. Sanchis, V., D. Lereclus, G. Menou, J. Chaufaux, S. Guo, and M. M. Lecadet. 1989. Nucleotide sequence and analysis of the N-terminal coding region of the Spodoptera-active δ-endotoxin gene of Bacillus thuringiensis var. aizawai 7.29. Mol. Microbiol. 3:229-238.
32. Sanchis, V., D. Lereclus, G. Menou, J. Chaufaux, and M. M. Lecadet. 1988. Multiplicity of δ-endotoxin genes with different insecticidal specificities in Bacillus thuringiensis aizawai 7.29. Mol. Microbiol. 2:393-404.
33. + efflux in vitro. J. Biol. Chem. 269:10088-10092.
34. Shapira, R., A. Ordentlich, I. Chet, and A. B. Oppenheim. 1989. Control of disease by chitinase expressed from cloned DNA in Escherichia coli. Phytopathology 79:1246-1249.
35. Smirnoff, W. A. 1977. Confirmation experimentales du potentiel du complexe Bacillus thuringiensis chitinase pour la repression de la tordeuse des bourgeons de espirette Choristoneura fuminerana. Can. Entomol. 109:351-358.
36. Smith, G. P., and D. J. Ellar. 1994. Mutagenesis of two surface-exposed loops of the Bacillus thuringiensis CryIC δ-endotoxin affects insecticidal specificity. Biochem. J. 302:611-616.
37. Smith, G. P., J. D. Merrick, E. J. Bone, and D. J. Ellar. 1996. Mosquitocidal activity of the CryIC δ-endotoxin from Bacillus thuringiensis subsp. aizawai. Appl. Environ. Microbiol. 62:680-684.
38. Sneh, B., B. F. Holdaway, G. R. Hooper, and J. L. Lockwood. 1976. Germination-lysis, as a mechanism of biological control of Thielaviopsis basicola pathogenic on soybeans. Can. J. Bot. 54:1499-1508.
39. Sneh, B., S. Schuster, and S. Gross. 1983. Improvement of the insecticidal activity of Bacillus thuringiensis var. entomocidus on larvae of Spodoptera littoralis (Lepidoptera, Noctuidae) by addition of chitinolytic bacteria, a phagostimulant and a UV-protectant. Z. Angew. Entomol. 96:77-83.
40. Stahly, C. P., D. W. Dingman, L. A. Bulla, Jr., and A. I. Aronson. 1978. Possible origin and function of the parasporal crystals in Bacillus thuringiensis. Biochem. Biophys. Res. Commun. 84:581-588.
41. Strizhov, N., M. Keller, Z. Konez-Kalman, A. Regev, B. Sneh, J. Schell, C. Koncz, and A. Zilberstein. Mapping of the entomocidal fragment of spodoptera-specific Bacillus thuringiensis toxin CryIC. Mol. Gen. Genet., in press.
42. Studier, W. F., A. H. Rosenberg, J. J. Dan, and J. W. Dubendorf. 1990. Use of T7 RNA polymerase to direct expression of cloned genes. Methods Enzymol. 185:60-89.
43. Tabashnik, B. E. 1994. Evolution of resistance to Bacillus thuringiensis. Annu. Rev. Entomol. 39:47-79.
44. Tabashnik, B. E., N. Finson, F. R. Groeters, W. J. Moar, M. W. Johnson, K. Luo, and M. J. Adang. 1994. Reversal of resistance to Bacillus thuringiensis in Plutella xylostella. Proc. Natl. Acad. Sci. USA 91:4120-4124.
45. Tabashnik, B. E., N. Finson, M. W. Johnson, and W. J. Moar. 1993. Resistance to toxins from Bacillus thuringiensis subsp. kurstaki causes minimal cross-resistance to B. thuringiensis subsp. aizawai in the diamondback moth (Lepidoptera: Plutellidae). Appl. Environ. Microbiol. 59:1332-1335.
46. Terra, W. R. 1990. Evolution of digestive systems of insects. Annu. Rev. Entomol. 35:181-200.
47. Vaeck, M., A. Reynaerts, H. Hofte, S. Jansens, M. De Beukeleer, C. Dean, M. Sabeau, M. Van Montague, and J. Leeman. 1987. Transgenic plants protected from insect attack. Nature (London) 328:33-37.
48. Van Rie, J., S. Jansens, H. Höfte, D. Degheele, and H. Van Mellaert. 1990. Receptors on the brush border membrane of the insect midgut as determinants of the specificity of Bacillus thuringiensis delta-endotoxins. Appl. Environ. Microbiol. 56:1378-1385.
49. Visser, B., E. Munsterman, A. Stoker, and W. G. Dirkse. 1990. A novel Bacillus thuringiensis gene encoding a Spodoptera exigua-specific crystal protein. J. Bacteriol. 172:6783-6788.
50. Wider, W. R., and H. R. Whiteley. 1990. Location of the dipteran specificity region in a lepidopteran-dipteran crystal protein from Bacillus thuringiensis. J. Bacteriol. 172:2826-2832.
51. Wu, D., and A. I. Aronson. 1992. Localized mutagenesis defines regions of the Bacillus thuringiensis δ-endotoxin involved in toxicity and specificity. J. Biol. Chem. 267:2311-2317.
52. Wu, S.-J., and D. H. Dean. 1996. Functional significance of loops in the receptor binding domain of Bacillus thuringiensis CryIIIA δ-endotoxin. J. Mol. Biol. 255:628-640.
53. Wunn, J., A. Kloti, P. K. Burkhardt, G. C. G. Biswas, K. Launis, V. A. Iglesias, and I. Potrykus. 1996. Transgenic Indica rice breeding line IR58 expressing a synthetic cryIA(b) gene from Bacillus thuringiensis provides effective insect pest control. Bio/technology 14:171-176.
54. Yunovitz, H., B. Sneh, and A. Yawetz. 1986. A new sensitive method for determining the toxicity of a highly purified fraction from δ-endotoxin produced by Bacillus thuringiensis var. entomocidus on isolated larval midgut of Spodoptera littoralis (Lepidoptera, Noctuidae). J. Invertebr. Pathol. 48:223-231.