Measurement of cysteine dioxygenase activity and protein abundance

Current Protocols in Toxicology

Volumn , Issue SUPPL. 38, 2008, Pages

  Stipanuk, Martha H ^a   Dominy Jr , John E ^a   Ueki, Iori ^a   Hirschberger, Lawrence L ^a  

^a Department of Obstetrics Gynecology   (United States)

Author keywords

Cysteine; Cysteine dioxygenase; Cysteinesulfinic acid; Sulfate; Taurine

Indexed keywords

CYSTEINE DIOXYGENASE; CYSTEINE SULFINIC ACID;

ANIMAL CELL; ANIMAL TISSUE; CATALYSIS; CHEMICAL REACTION; CROSS LINKING; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME ISOLATION; ENZYME PURIFICATION; ENZYME SYNTHESIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IMMUNOBLOTTING; ISOTOPE LABELING; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; REVIEW;

EID: 63849233050     PISSN: 19349254     EISSN: 19349262     Source Type: Journal    
DOI: 10.1002/0471140856.tx0615s38     Document Type: Review

References (35)

1. Bagley, P.J. and Stipanuk, M.H. 1994. The activities of hepatic cysteine dioxygenase and cysteinesulfinate decarboxylase are regulated in a reciprocal manner in response to dietary casein level in rats. J. Nutr. 124:2410-2421.
2. Bagley, P.J., Hirschberger, L.L., and Stipanuk, M.H. 1995. Evaluation and modification of an assay procedure for cysteine dioxygenase activity: High-performance liquid chromatography method for measurement of cysteine sulfinate and demonstration of physiological relevance of cysteine dioxygenase activity in cysteine catabolism. Anal. Biochem. 227:40-48.
3. Bella, D.L., Kwon, Y.H., and Stipanuk, M.H. 1996. Variations in dietary protein but not in dietary fat plus cellulose or carbohydrate levels affect cysteine metabolism in rat isolated hepatocytes. J. Nutr. 126:2179-2187.
4. Bella, D.L., Hirschberger, L.L., Hosokawa, Y., and Stipanuk, M.H. 1999. Themechanisms involved in the regulation of key enzymes of cysteine metabolism in rat liver in vivo. Am. J. Physiol. 276:E326-E335.
5. Coloso, R.M., Drake, M.R., and Stipanuk, M.H. 1990. Effect of bathocuproine disulfonate, a copper chelator, on cyst(e)ine metabolism by freshly isolated rat hepatocytes. Am. J. Physiol. 259:E443-E450.
6. Daniels, K.M. and Stipanuk, M.H. 1982. The effect of dietary cysteine level on cysteine metabolism in rats. J. Nutr. 112:2130-2141.
7. de la Rosa, J. and Stipanuk, M.H. 1985. Evidence for a rate-limiting role of cysteinesulfinate decarboxylase activity in taurine biosynthesis in vivo. Comp. Biochem. Physiol. B. 81:565-571.
8. Dominy, J.E. Jr., Hirschberger, L.L., Coloso, R.M., and Stipanuk, M.H. 2006a. Regulation of cysteine dioxygenase degradation is mediated by intracellular cysteine levels and the ubiquitin-26S proteasome system in the living rat. Biochem. J. 394:267-273.
9. Dominy, J.E. Jr., Simmons, C.R., Karplus, P.A., Gehring, A.M., and Stipanuk, M.H. 2006b. Identification and characterization of bacterial cysteine dioxygenases: A new route of cysteine degradation for eubacteria. J. Bacteriol. 188:5561-5569.
10. Dominy, J.E. Jr., Hwang, J., and Stipanuk, M.H. 2007. Overexpression of cysteine dioxygenase reduces intracellular cysteine and glutathione pools in HepG2/C3A cells. Am. J. Physiol. Endocrinol. Metab. 293:E62-E69.
11. Dominy, J.E. Jr., Hwang, J., Guo, S., Hirschberger, L.L., Zhang, S., and Stipanuk, M.H. 2008. Synthesis of amino acid cofactor in cysteine dioxygenase is regulated by substrate and represents a novel post-translational regulation of activity. J. Biol. Chem. 283:12188-12201.
12. Gallagher, S., Winston, S.E., Fuller, S.A., and Hurrell, J.G.R. 2008. Immunoblotting and immunodetection. Curr. Protoc. Mol. Biol. 83:10.8.1-10.8.28.
13. Hirschberger, L.L., Daval, S., Stover, P.J., and Stipanuk, M.H. 2001. Murine cysteine dioxygenase gene: Structural organization, tissue-specific expression and promoter identification. Gene 277:153-161.
14. Hosokawa, Y., Matsumoto, A., Oka, J., Itakura, H., and Yamaguchi, K. 1990. Isolation and characterization of a cDNA for rat liver cysteine dioxygenase. Biochem. Biophys. Res. Commun. 168:473-478.
15. Kuo, S.M. and Stipanuk, M.H. 1984. Changes in cysteine dioxygenase and cysteinesulfinate decarboxylase activities and taurine levels in tissues of pregnant or lactating rat dams and their fetuses or pups. Biol. Neonate. 46:237-248.
16. Kwon, Y.H. and Stipanuk, M.H. 2001. Cysteine regulates cysteine dioxygenase and γ-glutamylcysteine synthetase in cultured rat hepatocytes. Am. J. Physiol. 280:E804-E815.
17. Lee, J.-I., Londono, M.P., Hirschberger, L.L., and Stipanuk, M.H. 2004. Regulation of cysteine dioxygenase and γ-glutamylcysteine synthetase is associated with hepatic cysteine level. J. Nutr. Biochem. 15:112-122.
18. Lee, J-I., Kang, J., and Stipanuk, M.H. 2006. Differential regulation of glutamate-cysteine ligase subunit expression and increased holoenzyme formation in response to cysteine deprivation. Biochem. J. 393:181-190.
19. McCoy, J.G., Bailey, L.J., Bitto, E., Bingman, C.A., Aceti, D.J., Fox, B.G., and Phillips, G.N. Jr. 2006. Structure and mechanism of mouse cysteine dioxygenase. Proc. Natl. Acad. Sci. U.S.A. 103:3084-3089.
20. Ohta, J., Kwon, Y.-H., and Stipanuk, M.H. 2000. Cysteine metabolism by primary cultures of hepatocytes responds to methionine supplementation of the culture medium. Amino Acids 19:705-728.
21. Parsons, R.B., Barber, P.C., Waring, R.H., Williams, A.C., and Ramsden, D.B. 1998a. Cysteine dioxygenase: Regional expression of activity in rat brain. Neurosci. Lett. 248:101-104.
22. Parsons, R.B., Ramsden, D.B., Waring, R.H., Barber, P.C., and Williams, A.C. 1998b. Hepatic localisation of rat cysteine dioxygenase. J. Hepatol. 29:595-602.
23. Parsons, R.B., Sampson, D., Huggins, C.C., Waring, R.H., Williams, A.C., and Ramsden, D.B. 2001a. Renal localisation of rat cysteine dioxygenase. Nephron 88:340-346.
24. Parsons, R.B., Waring, R.H., Williams, A.C., and Ramsden, D.B. 2001b. Cysteine dioxygenase: Regional localisation of protein and mRNA in rat brain. J. Neurosci. Res. 65:78-84.
25. Qusti, S., Parsons, R.B., Abouglila, K.D., Waring, R.H., Williams, A.C., and Ramsden, D.B. 2000. Development of an in vitro model for cysteine dioxygenase expression in the brain. Cell Biol. Toxicol. 16:243-255.
26. Simmons, C.R., Liu, Q., Huang, Q., Hao, Q., Begley, T.P., Karplus, P.A., and Stipanuk, M.H. 2006a. Crystal structure of mammalian cysteine dioxygenase: A novel mononuclear iron center for cysteine thiol oxidation. J. Biol. Chem. 281:18723-18733.
27. Simmons, C.R., Hirschberger, L.L., Machi, M.S., and Stipanuk, M.H. 2006b. Expression, purification, and kinetic characterization of recombinant rat cysteine dioxygenase, a non-heme metalloenzyme necessary for regulation of cellular cysteine levels. Protein Expr. Purif. 47:74-81.
28. Stipanuk, M.H. and Beck, P.W. 1982 Characterization of the enzymic capacity for cysteine desulphhydration in liver and kidney of the rat. Biochem. J. 206:267-277.
29. Stipanuk, M.H., Londono, M., Lee, J.-I., Hu, M., and Yu, A.F. 2002. Enzymes and metabolites of cysteine metabolism in nonhepatic tissues of rats showlittle response to changes in dietary protein or sulfur amino acid levels. J. Nutr. 132:3369-3378.
30. Stipanuk, M.H., Londono, M., Hirschberger, L.L., Wang, L., and Hickey, C. 2004a. Evidence for expression of a single distinct form of mammalian cysteine dioxygenase. Amino Acids 26:99-106.
31. Stipanuk, M.H., Hirschberger, L.L., Londono, M.P., Cresenzi, C.L., and Yu, A.F. 2004b. The ubiquitin-proteasome system is responsible for cysteine-responsive regulation of cysteine dioxygenase concentration in liver. Am. J. Physiol. Endocrinol. Metab. 286:E439-E448.
32. Stipanuk, M.H., Dominy, J.E. Jr., Lee, J.I., and Coloso, R.M. 2006. Mammalian cysteine metabolism: Newinsights into regulation of cysteine metabolism. J. Nutr. 136:1652S-1659S.
33. Ueki, I. and Stipanuk, M.H. 2007. Enzymes of the taurine biosynthetic pathway are expressed in rat mammary gland. J. Nutr. 137:1887-1894.
34. Yamaguchi, K., Hosokawa, Y., Kohashi, N., Kori, Y., Sakakibara, S., and Ueda, I. 1978. Rat liver cysteine dioxygenase (cysteine oxidase): Further purification, characterization, and analysis of the activation and inactivation. J. Biochem. 83:479-491.
35. Ye, S., Wu, X., Wei, L., Tang, D., Sun, P., Bartlam, M., and Rao, Z. 2007. An insight into the mechanism of human cysteine dioxygenase: Key roles of the thioether-bonded tyrosine-cysteine cofactor. J. Biol. Chem. 282:3391-3402.