Detection of Cryptosporidium spp. from human faeces by PCR-RFLP, cloning and sequencing

Parasitology Research

Volumn 104, Issue 3, 2009, Pages 583-587

  Leone, Annalisa ^a   Ripabelli, Giancarlo ^a   Sammarco, Michela Lucia ^a   Grasso, Guido Maria ^a  

^a UNIVERSITY OF MOLISE   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; CONTROLLED STUDY; CRYPTOSPORIDIUM; CRYPTOSPORIDIUM HOMINIS; CRYPTOSPORIDIUM PARVUM; GENE INSERTION; GENE SEQUENCE; GENOTYPE; MOLECULAR CLONING; NONHUMAN; PARASITE IDENTIFICATION; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RESTRICTION FRAGMENT LENGTH POLYMORPHISM; ANIMAL; CLASSIFICATION; CRYPTOSPORIDIOSIS; DNA FINGERPRINTING; DNA SEQUENCE; EVALUATION; FECES; GENETICS; HUMAN; ISOLATION AND PURIFICATION; METHODOLOGY; PARASITOLOGY; SENSITIVITY AND SPECIFICITY; UNITED KINGDOM;

CRYPTOSPORIDIUM; CRYPTOSPORIDIUM HOMINIS; CRYPTOSPORIDIUM MELEAGRIDIS; CRYPTOSPORIDIUM PARVUM;

OOCYST WALL PROTEIN, CRYPTOSPORIDIUM; PROTOZOAL DNA; PROTOZOAL PROTEIN;

ANIMALS; CLONING, MOLECULAR; CRYPTOSPORIDIOSIS; CRYPTOSPORIDIUM; DNA FINGERPRINTING; DNA, PROTOZOAN; ENGLAND; FECES; GENOTYPE; HUMANS; POLYMERASE CHAIN REACTION; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; PROTOZOAN PROTEINS; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA;

EID: 63849112902     PISSN: 09320113     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00436-008-1233-8     Document Type: Article

References (24)

1. SF Altschul W Gish W Miller EW Myers DJ Lipman 1990 Basic local alignment search tool J Mol Biol 215 403 410
2. CF Amar PH Dear J McLauchlin 2004 Detection and identification by real time PCR/RFLP analyses of Cryptosporidium species from human faeces Lett Appl Microbiol 38 217 222
3. Anonymous 1999 Surveillance of waterborne disease and water quality: January to June 1998 Public Health Lab Serv Commun Dis Rep 9 73 74
4. Anonymous 1999 Surveillance of waterborne disease and water quality. January to June 1999, and summary 1999 Public Health Lab Serv Commun Dis Rep 9 305 308
5. Anonymous 2000 Surveillance of waterborne disease and water quality: July to December 1999 Public Health Lab Serv Commun Dis Rep 10 65 68
6. R Boom CJA Sol MMM Salimans CL Jansen PME Wertheim van Dillen J van der Noordaa 1990 Rapid and simple method for purification of nucleic acids J Clin Microbiol 28 495 503
7. SM Cacciò RCA Thompson J McLauchlin HV Smith 2005 Unravelling Cryptosporidium and Giardia epidemiology Trends Parasitol 21 430 437
8. VA Cama C Bern IM Sulaiman RH Gilman E Ticona A Vivar V Kawai D Vargas L Zhou L Xiao 2003 Cryptosporidium species and genotypes in HIV-positive patients in Lima, Peru J Eukaryot Microbiol 50 531 533
9. M Carraway S Tzipori G Widmer 1997 A new restriction fragment length polymorphism from Cryptosporidium parvum identifies genetic heterogeneous parasite populations and genetic changes following transmission from bovine to human hosts Infect Immun 65 3958 3960
10. D Dawson 2005 Foodborne protozoan parasites Int J Food Microbiol 103 207 227
11. DC Feltus CW Giddings BL Schneck T Monson D Warshauer JM McEvoy 2006 Evidence supporting zoonotic transmission of Cryptosporidium spp. in Wisconsin J Clin Microbiol 44 4303 4308
12. H Gòmez-Couso F Freire-Santos CF Amar KA Grant K Williamson ME Ares-Mazas J McLauchlin 2004 Detection of Cryptosporidium and Giardia in molluscan shellfish by multiplexed nested-PCR Int J Food Microbiol 91 279 278
13. H Gòmez-Couso CFL Amar J McLauchlin E Ares-Mazás 2005 Characterisation of a Cryptosporidium isolate from water buffalo (Bubalus bubalis) by sequencing of a fragment of the Cryptosporidium oocyst wall protein gene (COWP) Vet Parasitol 131 139 144
14. A Hashimoto S Hitomi M Shigemitsu H Tsuyoshi 2006 Genotyping of single Cryptosporidium oocysts in sewage by semi-nested PCR and direct sequencing Water Res 40 2527 2532
15. J McLauchlin S Pedraza-Díaz C Amar-Hoetzeneder GL Nichols 1999 Genetic characterization of Cryptosporidium strains from 218 patients with diarrhea diagnosed as having sporadic cryptosporidiosis J Clin Microbiol 37 3153 3158
16. J McLauchlin C Amar S Pedraza-Díaz GL Nichols 2000 Molecular epidemiological analysis of Cryptosporidium spp. in the United Kingdom: results of genotyping Cryptosporidium spp. in 1,705 fecal samples from humans and 105 fecal from livestock animals J Clin Microbiol 38 3984 3990
17. S Pedraza-Dìaz C Amar GL Nichols J McLauchlin 2001 Nested polymerase chain reaction for amplification of the Cryptosporidium oocyst wall protein gene Emerg Infect Dis 7 49 56
18. A Samie PO Bessong CL Obi JE Sevilleja S Stroup E Houpt RL Guerrant 2006 Cryptosporidium species: preliminary descriptions of the prevalence and genotype distribution among school children and hospital patients in the Venda region, Limpopo Province, South Africa Exp Parasitol 114 314 322
19. GD Sturbaum C Reed PJ Hoover BH Jost MM Marshall CR Sterling 2001 Species-specific, nested PCR-restriction fragment length polymorphism detection of single Cryptosporidium parvum oocysts Appl Environ Microbiol 67 2665 2668
20. IM Sulaiman L Xiao AA Lal 1999 Evaluation of Cryptosporidium parvum genotyping techniques Appl Environ Microbiol 65 4431 4435
21. O Sunnotel CJ Lowery JE Moore JSG Dooley L Xiao BC Millar PJ Rooney WJ Snelling 2006 Cryptosporidium Lett Appl Microbiol 1 7 16
22. LA Trotz-Williams DS Martin W Gatei V Cama AS Peregrine SW Martin DV Nydam F Jamieson L Xiao 2006 Genotype and subtype analyses of Cryptosporidium isolates from dairy calves and humans in Ontario Parasitol Res 99 346 352
23. G Widmer 1998 Genetic heterogeneity and PCR detection of Cryptosporidium parvum Adv Parasitol 40 223 239
24. L Xiao R Fayer U Ryan SJ Upton 2004 Cryptosporidium taxonomy: recent advances and implications for public health Clin Microbiol Rev 17 72 97