A pseudo-phytochelatin synthase in the ciliated protozoan Tetrahymena thermophila

Comparative Biochemistry and Physiology - C Toxicology and Pharmacology

Volumn 149, Issue 4, 2009, Pages 598-604

  Amaro, Francisco ^a   Ruotolo, Roberta ^b   Martín González, Ana ^a   Faccini, Andrea ^b   Ottonello, Simone ^b   Gutiérrez, Juan Carlos ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b UNIVERSITY OF PARMA   (Italy)

Author keywords

Gene expression; Heavy metals; Pseudo phytochelatin synthase; Tetrahymena thermophila

Indexed keywords

CADMIUM; CYSTEINE; GLUTATHIONE; PHYTOCHELATIN; PHYTOCHELATIN SYNTHASE; PSEUDOPHYTOCHELATIN SYNTHASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; ENZYME ANALYSIS; HYDROLYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE ANALYSIS; STRESS; TETRAHYMENA THERMOPHILA; UPREGULATION;

CILIOPHORA; CYANOBACTERIA; EUKARYOTA; MYXOZOA; TETRAHYMENA THERMOPHILA;

EID: 63449120927     PISSN: 15320456     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.cbpc.2009.01.002     Document Type: Article

References (38)

1. Amaro F., de Lucas M.P., Martín-González A., and Gutiérrez J.C. Two new members of the Tetrahymena multi-stress-inducible metallothionein family: characterization and expression analysis of T. rostrata Cd/Cu metallothionein genes. Gene 423 (2008) 85-91
2. Beck A., Lendzian K., Oven M., Christmann A., and Grill E. Phytochelatin synthase catalyzes key step in turnover of glutathione conjugates. Phytochemistry 62 (2003) 423-431
3. Blum R., Beck A., Korte A., Stengel A., Letzel T., Lendzian K., and Grill E. Function of phytochelatin synthase in catabolism of glutathione-conjugates. Plant J. 49 (2007) 740-749
4. Boldrin F., Santovito G., Irato P., and Piccinni E. Metal interaction and regulation of Tetrahymena pigmentosa metallothionein genes. Protist 153 (2002) 283-291
5. Brulle F., Cocquerelle C., Wamalah A.N., Morgan A.J., Kilee P., Lepretre A., and Vandenbulcke F. cDNA cloning and expression analysis of Eisenia fetida (Annelida: Oligochaeta) phytochelatin synthase under cadmium exposure. Ecotoxicol. Environ. Safety 71 (2008) 47-55
6. Clemens S., and Simm C. Schizosaccharomyces pombe as a model for metal homeostasis in plant cells: the phytochelatin-dependent pathway is the main cadmium detoxification mechanism. New Phytol. 159 (2003) 323-330
7. Clemens S., Kim E.J., Neumann D., and Schroeder J.I. Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast. Embo J. 18 (1999) 3325-3333
8. Clemens S., Schroeder J., and Degenkolb T. Caenorhabditis elegans expresses a functional phytochelatin synthase. Eur. J. Biochem. 268 (2001) 3640-3643
9. Cobbett C.S. Phytochelatin biosynthesis and function in heavy-metal detoxification. Curr. Opin. Plant Biol. 3 (2000) 211-216
10. Cobbett C.S., and Goldsbrough P. Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis. Annu. Rev. Plant Biol. 53 (2002) 159-182
11. Courbot M., Diez L., Ruotolo R., Chalot M., and Leroy P. Cadmium-responsive thiols in the ectomycorrhizal fungus Paxillus involutus. Appl. Environ. Microbiol. 70 (2004) 7413-7417
12. De Knecht J., Van Dillen M., Koevoets P., Schat H., Verkleij J., and Ernst W. Phytochelatins in cadmium-sensitive and cadmium-tolerant Silene vulgaris. Plant Physiol. 104 (1994) 255-261
13. Díaz S., Amaro F., Rico D., Campos V., Benítez L., Martín-González A., Hamilton E.P., Orias E., and Gutiérrez J.C. Tetrahymena metallothioneins fall into two discrete subfamilies. PLoS ONE 2 (2007) e291
14. Gonzalez-Mendoza D., Quiroz A., and Zapata-Perez O. Coordinated responses of phytochelatin synthase and metallothionein genes in black mangrove, Avicennia germinans, exposed to cadmium and copper. Aquatic Toxicol. 83 (2007) 306-314
15. Grill E., Thumann J., Winnacker E.L., and Zenk M.H. Induction of heavy-metal binding phytochelatins by inoculation of cell cultures in standard media. Plant Cell Rep. 7 (1988) 375-378
16. Grill E., Loffler S., Winnacker E.L., and Zenk M.H. Phytochelatins, the heavy-metal-binding peptides of plants, are synthesized from glutathione by a specific gamma-glutamylcysteine dipeptidyl transpeptidase (phytochelatin synthase). Proc. Natl. Acad. Sci. U. S. A. 86 (1989) 6838-6842
17. Guindon S., and Gascuel O. A simple, fast and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst. Biology 52 (2003) 696-704
18. Guindon S., Lethiec F., Duroux P., and Gascuel O. Online-a web server for fast maximum likelihood-based phylogenetic inference. Nuc. Acids Res. 1 (2005) 33
19. Ha S.B., Smith A.P., Howden R., Dietrich W.M., Bugg S., O'Connell M.J., Goldsbrough P.B., and Cobbett C.S. Phytochelatin synthase genes from Arabidopsis and the yeast Schizosaccharomyces pombe. Plant Cell 11 (1999) 1153-1164
20. Hamilton E.P., and Orias E. Genetically mapping new mutants and cloned genes. Methods Cell Biol. 62 (2000) 219-228
21. Harada E., von Roepenack-Lahaye E., and Clemens S. A cyanobacterial protein with similarity to phytochelatin synthases catalyzes the conversion of glutathione to gamma-glutamylcysteine and lacks phytochelatin synthase activity. Phytochemistry 65 (2004) 3179-3185
22. Heiss S., Wachter A., Bogs J., Cobbett C.S., and Rausch T. Phytochelatin synthase (PCS) protein is induced in Brassica juncea leaves after prolonged Cd exposure. J. Exp. Bot. 54 (2003) 1833-1839
23. Kondo N., Imai K., Isobe M., and Goto T. Cadystin A and B major unit peptides comprising cadmium binding peptides induced in a fission yeast - separation, revision of structures and synthesis. Tetrahedron Lett. 25 (1984) 3869-3872
24. Lee S., Moon J.S., Ko T.S., Petros D., Goldsbrough P.B., and Korban S.S. Overexpression of Arabidopsis phytochelatin synthase paradoxically leads to hypersensitivity to cadmium stress. Plant Physiol. 131 (2003) 656-663
25. Maier T., Yu C., Kullertz G., and Clemens S. Localization and functional characterization of metal-binding sites in phytochelatin synthases. Planta 218 (2003) 300-308
26. Notredame C., Higgins D., and Heringa J. A novel method for multiple sequence alignments. J. Mol. Biol. 302 (2000) 205-217
27. Pfaffl M.W. A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Res. 29 (2001) e45
28. Pfaffl M.W., Horgan G.W., and Dempfle L. Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acid Res. 30 (2002) e36
29. Ramos J., Clemente M.R., Naya L., Loscos J., Pérez-Rontome C., Sato S., Tabata S., and Becana M. Phytochelatin synthases of the model legume Lotus japonicus. A small multigene family with differential response to cadmium and alternatively spliced variants. Plant Physiol. 143 (2007) 1110-1118
30. Ramos J., Naya L., Gay M., Abian J., and Becana M. Functional characterization of an unusual phytochelatin synthase, LjPCS3, of Lotus japonicus. Plant Physiol. 148 (2008) 536-545
31. Rea P.A., Vatamaniuk O.K., and Rigden D.J. Weeds, worms, and more. Papain's long-lost cousin, phytochelatin synthase. Plant Physiol. 136 (2004) 2463-2474
32. Ruotolo R., Peracchi A., Bolchi A., Infusini G., Amoresano A., and Ottonello S. Domain organization of phytochelatin synthase: functional properties of truncated enzyme species identified by limited proteolysis. J. Biol. Chem. 279 (2004) 14686-14693
33. Tsuji N., Nishikori S., Iwabe O., Shiraki K., Miyasaka H., Takagi M., Hirata K., and Miyamoto K. Characterization of phytochelatin synthase-like protein encoded by alr0975 from a prokaryote, Nostoc sp. PCC 7120. Biochem. Biophys. Res. Commun. 315 (2004) 751-755
34. Tsuji N., Nishikori S., Iwabe O., Matsumoto S., Shiraki K., Miyasaka H., Takagi M., Miyamoto K., and Hirata K. Comparative analysis of the two-step reaction catalyzed by prokaryotic and eukaryotic phytochelatin synthase by an ion-pair liquid chromatography assay. Planta 222 (2005) 181-191
35. Vatamaniuk O.K., Mari S., Lu Y.P., and Rea P.A. AtPCS1, a phytochelatin synthase from Arabidopsis: isolation and in vitro reconstitution. Proc. Natl. Acad. Sci. U. S. A. 96 (1999) 7110-7115
36. Vatamaniuk O.K., Bucher E.A., Ward J.T., and Rea P.A. A new pathway for heavy metal detoxification in animals. Phytochelatin synthase is required for cadmium tolerance in Caenorhabditis elegans. J. Biol. Chem. 276 (2001) 20817-20820
37. Vestergaard M., Matsumoto S., Nishikori S., Shiraki K., Hirata K., and Takagi M. Chelation of cadmium ions by phytochelatin synthase: role of the cystein-rich C-terminal. Anal. Sci. 24 (2008) 277-281
38. Zhang H., Xu W., Guo J., He Z., and Ma M. Coordinated responses of phytochelatins and metallothioneins to heavy metals in garlic seedlings. Plant Sci. 169 (2005) 1059-1065