Characterization of phytochelatin synthase-like protein encoded by alr0975 from a prokaryote, Nostoc sp. PCC 7120

Biochemical and Biophysical Research Communications

Volumn 315, Issue 3, 2004, Pages 751-755

  Tsuji, Naoki ^a   Nishikori, Shingo ^b   Iwabe, Osamu ^a   Shiraki, Kentaro ^b   Miyasaka, Hitoshi ^c   Takagi, Masahiro ^b   Hirata, Kazumasa ^a   Miyamoto, Kazuhisa ^a  

^a OSAKA UNIVERSITY   (Japan)

^b JAPAN ADVANCED INSTITUTE OF SCIENCE AND TECHNOLOGY   (Japan)

^c KANSAI ELECTRIC POWER CO   (Japan)

Author keywords

Cyanobacterium; Heavy metal; Nostoc sp. PCC 7120; Phytochelatin synthase; Phytochelatins; Glutamylcysteine

Indexed keywords

GAMMA GLUTAMYLCYSTEINE; GLUTATHIONE; PHYTOCHELATIN SYNTHASE; RECOMBINANT PROTEIN;

ALGA; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CATALYSIS; CATALYST; CONTROLLED STUDY; CYANOBACTERIUM; DETOXIFICATION; EUKARYOTE; GENE SEQUENCE; GENETIC CODE; GENETIC IDENTIFICATION; GENETIC VARIABILITY; GENOME; NONHUMAN; NOSTOC; PRIORITY JOURNAL; PROKARYOTE; PROTEIN ANALYSIS; PROTEIN SYNTHESIS;

ALGAE; EMBRYOPHYTA; EUKARYOTA; FUNGI; NOSTOC; NOSTOC SP.; NOSTOC SP. PCC 7120; PROKARYOTA;

EID: 1242272837     PISSN: 0006291X     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.bbrc.2004.01.122     Document Type: Article

References (18)

1. Gekeler W., Grill E., Winnacker E.-L., Zenk M.H. Algae sequester heavy metals via synthesis of phytochelatin complexes. Arch. Microbiol. 150:1988;197-202.
2. Gekeler W., Grill E., Winnacker E.-L., Zenk M.H. Survey of the plant kingdom for the ability to bind heavy metals through phytochelatins. Z. Naturforsch. 44c:1989;361-369.
3. Grill E., Winnacker E.-L., Zenk M.H. Phytochelatins: the principal heavy-metal complexing peptides of plants. Science. 230:1985;674-676.
4. Grill E., Löffler S., Winnacker E.-L., Zenk M.H. Phytochelatins, the heavy-metal binding peptides of plants, are synthesized from glutathione by specific γ-glutamylcysteine dipeptidyl transpeptidase (phytochelatin synthase). Proc. Natl. Acad. Sci. USA. 86:1989;6838-6842.
5. Clemens S., Kim E.J., Neumann D., Chroeder J.I. Tolerance to toxic metals by a gene family of phytochelatin synthases from plants and yeast. EMBO J. 18:1999;3325-3333.
6. Ha S.-B., Smith A.P., Howden R., Dietrich W.M., Bugg S., O'Connell M.J., Goldsbrough P.B., Cobbett C.S. Phytochelatin synthase genes from Arabidopsis and the yeast Schizosaccharomyces pombe. Plant Cell. 11:1999;1153-1163.
7. Vatamaniuk O.K., Mari S., Lu Y.-P., Rea P.A. AtPCS1, a phytochelatin synthase from Arabidopsis: isolation and in vitro reconstitution. Proc. Natl. Acad. Sci. USA. 96:1999;7110-7115.
8. Vatamaniuk O.K., Bucher E.A., Ward J.T., Rea P.A. A new pathway for heavy metal detoxification in animals. J. Biol. Chem. 276:2001;20817-20820.
9. Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S.et al. Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions. DNA Res. 3:1996;109-136.
10. Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A., Iriguchi M., Ishikawa A., Kawashima K., Kimura T.et al. Complete genomic sequence of the filamentous nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120. DNA Res. 8:2001;205-213.
11. Nakamura Y., Kaneko T., Sato S., Ikeuchi M., Katoh H., Sasamoto S., Watanabe A., Iriguchi M., Kawashima K., Kimura T.et al. Complete genome structure of the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1. DNA Res. 9:2002;123-130.
12. Bradford M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:1976;248-254.
13. Oven M., Page J.E., Zenk M.H., Kutchan T.M. Molecular characterization of the homo-phytochelatin synthase of soybean Glycine max: relation to phytochelatin synthase. J. Biol. Chem. 277:2002;4747-4754.
14. Hirata K., Tsujimoto Y., Namba T., Ohta T., Hirayanagi N., Miyasaka H., Zenk M.H., Miyamoto K. Strong induction of phytochelatin synthesis by Zn in marine green alga, Dunaliella tertiolacta. J. Biosci. Bioeng. 92:2001;24-29.
15. Vatamaniuk O.K., Mari S., Lu Y.-P., Rea P.A. Mechanism of heavy metal ion activation of phytochelatin (PC) synthase. J. Biol. Chem. 275:2000;31451-31459.
16. Cobbett C., Goldsbrough P. Phytochelatins and metallothioneins: roles in heavy metal detoxification and homeostasis. Annu. Rev. Plant Biol. 53:2002;159-182.
17. Zenk M.H. Heavy metal detoxification in higher plants - a review. Gene. 179:1996;21-30.
18. Maier T., Yu C., Küllertz G., Clemens S. Localization and functional characterization of metal-binding sites in phytochelatin synthases. Planta. 218:2003;300-308.