Purification and characterization of human brain serine racemase expressed in moderately halophilic bacteria

Protein and Peptide Letters

Volumn 16, Issue 2, 2009, Pages 201-206

  Nagayoshi, Chizuru ^a   Ishibashi, Matsujiro ^a   Tokunaga, Masao ^a  

^a KAGOSHIMA UNIVERSITY   (Japan)

Author keywords

groE; Halophile; His tag; Human serine racemase; Porin

Indexed keywords

DIVALENT CATION; DRUG DERIVATIVE; ISOMERASE; PORIN; SERINE; SERINE O-SULFATE; SERINE RACEMASE; SERINE SULFATE;

AFFINITY CHROMATOGRAPHY; ARTICLE; BRAIN; CHEMISTRY; ENZYMOLOGY; GENETICS; HALOMONAS; HUMAN; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROMOTER REGION; PROTEIN STABILITY;

BASE SEQUENCE; BRAIN; CATIONS, DIVALENT; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; HALOMONAS; HUMANS; MOLECULAR SEQUENCE DATA; PORINS; PROMOTER REGIONS, GENETIC; PROTEIN STABILITY; RACEMASES AND EPIMERASES; SERINE;

BACTERIA (MICROORGANISMS);

EID: 63449118074     PISSN: 09298665     EISSN: None     Source Type: Journal    
DOI: 10.2174/092986609787316261     Document Type: Article

References (30)

1. Hashimoto, A., Nishikawa, T., Hayashi, T., Fujii, N., Harada, K., Oka, T. and Takahashi, K. The presence of free D-serine in rat brain. (1992) FEBS Lett., 296, 33-36.
2. Mothet, J. P., Parent, A. T., Wolosker, H., Brady, Jr. R. O., Linden, D. J., Ferris, C. D., Rogawski, M. A. and Snyder, S. H. D-serine is an endogenous ligand for the glycine site of the N-methyl-D-aspartate receptor. (2000) Proc. Natl. Acad. Sci. USA, 97, 4926-4931.
3. Stevens, E. R., Esguerra, M., Kim, P. M., Newman, E. A., Snyder, S. H., Zahs, K. R. and Miller, R. F. D-serine and serine racemase are present in the vertebrate retina and contribute to the physiological activation of NMDA receptors. (2003) Proc. Natl. Acad. Sci. USA, 100, 6789-6794.
4. Wolosker, H. NMDA receptor regulation by D-serine: new findings and perspectives. (2007) Mol. Neurobiol., 36, 152-164.
5. Shleper, M., Kartvelishvily, E. and Wolosker, H. D-serine is the dominant endogenous coagonist for NMDA receptor neurotoxicity in organotypic hippocampal slices. (2005) J. Neurosci., 25, 9413-9417.
6. Schell, M. J., Molliver, M. E. and Snyder, S. H. D-serine, an endogenous synaptic modulator: localization to astrocytes and glutamate-stimulated release. (1995) Proc. Natl. Acad. Sci. USA, 92, 3948-3952.
7. Wolosker, H., Sheth, K. N., Takahashi, M., Mothet, J. P., Brady, Jr., R. O., Ferris, C. D. and Snyder, S.H. Purification of serine racemase: biosynthesis of the neuromodulator D-serine. (1999) Proc. Natl. Acad. Sci. USA, 96, 721-725.
8. Kartvelishvily, E., Shleper, M., Balan, L., Dumin, E. and Wolosker, H. Neuron-derived D-serine release provides a novel means to activate N-methyl-D-aspartate receptors. (2006) J. Biol. Chem., 281, 14151-14162.
9. Yoshikawa, M., Nakajima, K., Takayasu, N., Noda, S., Sato, Y., Kawaguchi, M., Oka, T., Kobayashi, H. and Hashimoto, A. Expression of the mRNA and protein of serine racemase in primary cultures of rat neurons. (2006) Eur. J. Pharmacol., 548, 74-76.
10. Yoshikawa, M., Takayasu, N., Hashimoto, A., Sato, Y.,Tamaki, R., Tsukamoto, H., Kobayashi, H. and Noda, S. The serine racemase mRNA is predominantly expressed in rat brain neurons. (2007) Arch. Histol. Cytol., 70, 127-134.
11. Snyder, S. H. and Ferris, C. D. Novel neurotransmitters and their neuropsychiatrie relevance. (2000) Am. J. Psychiatry, 157, 1738-1751.
12. Kaneko, I., Morimoto, K. and Kubo, T. Drastic neuronal loss in vivo by beta-amyloid racemized at Ser(26) residue: conversion of non-toxic [D-Ser(26)] beta-amyloid 1-40 to toxic and proteinase-resistant fragments. (2001) Neuroscience, 104, 1003-1011.
13. Wu, S., Basile, A. S. and Barger, S. W. Induction of serine racemase expression and D-serine release from microglia by secreted amyloid precursor protein (sAPP). (2007) Curr. Alzheimer Res., 4, 243-251.
14. Nagayoshi, C., Ishibashi, M., Kita, Y., Matsuoka, M., Nishimoto, I. and Tokunaga, M. Expression, refolding and characterization of human brain serine racemase in Escherichia coli with N-terminal His-tag. (2005) Protein Pept. Lett., 12, 487-490.
15. Makrides, S. Strategies for achieving high-level expression of genes in Escherichia coli. (1996) Microbiol. Rev., 60, 512-538.
16. Ishibashi, M., Tsumoto, K., Tokunaga, M., Ejima, D., Kita, Y. and Arakawa, T. Is arginine a protein-denaturant? (2005) Protein Expr. Purif., 42, 1-6.
17. Singh, S. M. and Panda, A. K. Solubilization and refolding of bacterial inclusion body proteins. (2005) J. Biosci. Bioeng., 99, 303-310.
18. Ventosa, A., Metro, J. J. and Oren, A. Biology of moderately halophilic aerobic bacteria. (1998) Microbiol. Mol. Biol. Rev., 62, 504-544.
19. Galinski, E. A. Osmoadaptation in bacteria. (1995) Adv. Microb. Physiol., 37, 272-328.
20. Da Costa, M. S., Santos, H. and Galinski, E. A. An overview of the role and diversity of compatible solutes in Bacteria and Archaea. (1998) Adv. Biochem. Eng. Biotechnol., 61, 117-153.
21. Afendra, A. S., Vargas, C., Nieto, J. J. and Drainas, C. Gene transfer and expression of recombinant proteins in moderately halophilic bacteria. (2004) Methods Mol. Biol., 267, 209-223.
22. Tokunaga, H., Mitsuo, K., Kamekura, M. and Tokunaga, M. Major outer membrane proteins in moderately halophilic eubacteria of genera Chromohalobacter and Halomonas. (2004) J. Basic Microbiol, 44, 232-240.
23. Vargas, C., Coronado, M. J., Ventosa, A. and Nieto, J. J. Host range, stability and compatibility of broad host-range-plasmids and a shuttle vector in moderately halophilic bacteria. Evidence of intragenic and intergenic conjugation in moderate halophiles. (1997) Syst. Appl. Microbiol, 20, 173-181.
24. Nagayoshi, C., Tokunaga, H., Hayashi, A., Harazono, H., Hamasaki, K. ando, A. and Tokunaga, M. Efficient expression of haloarchaeal nucleoside diphosphate kinase via strong porin promoter in moderately halophilic bacteria. (2006) Protein Pept. Lett., 13, 611-615.
25. Panizzutti, R., Miranda, J. D., Ribeiro, C. S., Engelender, S. and Wolosker, H. A new strategy to decrease N-methyl-D-aspartate (NMDA) receptor coactivation: inhibition of D-serine synthesis by converting serine racemase into an eliminase. (2001) Proc. Natl. Acad. Sci. USA, 98, 5294-5299.
26. Tokunaga, M., Kato, S., Kawamura-Watabe, A., Tanaka, R. and Tokunaga, H. Characterization of deletion mutations in the carboxy-terminal peptide-binding domain of the Kar2 protein in Saccharomyces cerevisiae. (1998) Yeast, 14, 1285-1295.
27. Laemmli, U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. (1970) Nature, 227, 680-685.
28. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. J. Protein measurement with the Folin phenol reagent. (1951) J. Biol. Chem. 193, 265-275.
29. Yura, T., Nagai, H. and Mori, H. Regulation of the heat-shock response in bacteria. (1993) Annu. Rev. Microbiol, 47, 321-350.
30. Fujita, M., Amemura, A. and Aramaki, H. Transcription of the groESL Operon in Pseudomonas aeruginosa PAOl. (1998) FEMS Microbiol. Lett., 163, 237-242.