Construction of FLAG tagging vectors for Candida albicans

Yeast

Volumn 19, Issue 7, 2002, Pages 611-618

  Umeyama, Takashi ^a   Nagai, Yuki ^a   Niimi, Masakazu ^a   Uehara, Yoshimasa ^a  

^a NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

Author keywords

Candida albicans; FLAG epitope; Homologous recombination; Protein expression; Tagging

Indexed keywords

FLUCONAZOLE; FUNGAL DNA; FUNGAL PROTEIN; LUCIFERASE; METHIONINE;

ANTIBIOTIC RESISTANCE; ARTICLE; CANDIDA ALBICANS; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CULTURE MEDIUM; DNA VECTOR; GENE CASSETTE; GENE LOCUS; GENETIC ANALYSIS; HOMOZYGOSITY; MARKER GENE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; PROTEIN EXPRESSION; REPORTER GENE; WESTERN BLOTTING;

ACTINS; BASE SEQUENCE; CANDIDA ALBICANS; DNA, FUNGAL; EPITOPES; FLUCONAZOLE; FUNGAL PROTEINS; GENES, REPORTER; GENETIC VECTORS; LUCIFERASES; MEMBRANE TRANSPORT PROTEINS; MOLECULAR SEQUENCE DATA; NUCLEAR PROTEINS; PEPTIDES; PROMOTER REGIONS (GENETICS); SCHIZOSACCHAROMYCES POMBE PROTEINS; SEQUENCE TAGGED SITES; TRANSFORMATION, GENETIC;

CANDIDA; CANDIDA ALBICANS; RENILLA; RENILLA RENIFORMIS;

EID: 0036092195     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/yea.863     Document Type: Article

References (26)

1. Multiple efflux mechanisms are involved in Candida albicans fluconazole resistance
2. Evaluation of the CaMAL2 promoter for regulated expression of genes in Candida albicans
3. Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution
4. The MET3 promoter: A new tool for Candida albicans molecular genetics
5. Yeast-enhanced green fluorescent protein (yEGFP)-a reporter of gene expression in Candida albicans
6. Understanding, improving and using green fluorescent proteins
7. Recent developments in molecular genetics of Candida albicans
8. Morphogenesis-independent regulation of actin transcript levels in the pathogenic yeast Candida albicans
9. Isolation of monoclonal antibodies specific for human c-myc proto-oncogene product
10. Purification of a RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method
11. Isogenic strain construction and gene mapping in Candida albicans
12. Multicenter evaluation of a broth macrodilution antifungal susceptibility test for yeasts
13. Genetic transformation of yeast
14. Immunological approaches to the study of protein localisation in yeast
15. Induced expression of the Candida albicans multidrug resistance gene CDR1 in response to fluconazole and other antifungals
16. CIp10, an efficient and convenient integrating vector for Candida albicans
17. Tetracycline-regulatable system to tightly control gene expression in the pathogenic fungus Candida albicans
18. Candida species and virulence
19. Head and neck squamous cell growth suppression using adenovirus-p53-FLAG: A potential marker for gene therapy trials
20. A calcium-dependent antibody for identification and purification of recombinant proteins
21. Susceptibilities of Candida albicans multidrug transporter mutants to various antifungal agents and other metabolic inhibitors
22. Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione-S-transferase
23. The sea pansy Renilla reniformis luciferase serves as a sensitive bioluminescent reporter for differential gene expression in Candida albicans
24. Structure and regulation of a Candida albicans RP10 gene which encodes an immunogenic protein homologous to Saccharomyces cerevisiae ribosomal protein 10
25. Increased mRNA levels of ERG16, CDR, and MDR1 correlate with increases in azole resistance in Candida albicans isolates from a patient infected with human immunodeficiency virus
26. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors