Polymerization of purified yeast septins: Evidence that organized filament arrays may not be required for septin function

Journal of Cell Biology

Volumn 143, Issue 3, 1998, Pages 737-749

  Frazier, Jennifer A ^a   Wong, Mei Lie ^a   Longtine, Mark S ^b   Pringle, John R ^b   Mann, Matthias ^c   Mitchison, Timothy J ^d   Field, Christine ^d  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF NORTH CAROLINA   (United States)

^c EUROPEAN MOLECULAR BIOLOGY LABORATORY   (Germany)

^d HARVARD MEDICAL SCHOOL   (United States)

Author keywords

Cell division; Cytokinesis; Filaments; Saccharomyces cerevisiae; Septins

Indexed keywords

FUNGAL PROTEIN;

ARTICLE; ELECTRON MICROSCOPY; GENE DELETION; MASS SPECTROMETRY; NONHUMAN; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN POLYMERIZATION; PROTEIN PURIFICATION; YEAST;

CELL CYCLE PROTEINS; CYTOSKELETAL PROTEINS; FUNGAL PROTEINS; GTP PHOSPHOHYDROLASES; GTP-BINDING PROTEINS; MEMBRANE PROTEINS; POLYMERS; PROFILINS; RECOMBINANT FUSION PROTEINS; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SCHIZOSACCHAROMYCES POMBE PROTEINS; TRANSCRIPTION FACTORS;

EUKARYOTA; SACCHAROMYCES CEREVISIAE; SACCHAROMYCETALES;

EID: 14444286600     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.143.3.737     Document Type: Article

References (47)

1. Alberts, B., D. Bray, J. Lewis, M. Raff, K. Roberts, and J.D. Watson. 1994. Molecular Biology of the Cell. M. Richardson, editor. Garland Publishing, New York. 1,294 pp.
2. Amos, L., and W.B. Amos. 1991. Molecules of the Cytoskeleton. In Macmillan Molecular Biology Series. C. Skidmore, editor. Macmillan Education Ltd., London, UK. 193 pp.
3. Baudin, A., O. Ozier-Kalogeropoulos, A. Denouel, F. Lacroute, and C. Cullin. 1993. A simple and efficient method for direct gene deletion in Saccharomyces cerevisiae. Nucleic Acids Res. 21:5067-5076.
4. Bi, E., and J.R. Pringle. 1996. ZDS1 and ZDS2, genes whose products may regulate Cdc42p in Saccharomyces cerevisiae. Mol. Cell. Biol. 16:5264-5275.
5. Bourne, H.R., D.A. Sanders, and F. McCormick. 1991. The GTPase superfamily: conserved structure and molecular mechanism. Nature. 349:117-127.
6. Byers, B., and L. Goetsch. 1976. A highly ordered ring of membrane-associated filaments in budding yeast. J. Cell Biol. 69:717-721.
7. Byers, B., and L. Goetsch. 1991. Preparation of yeast cells for thin-section electron microscopy. Methods Enzymol. 194:602-608.
8. Carroll, C.W., R. Altman, D. Schieltz, J. Yates, and D. Kellogg. 1998. The septins are required for the mitosis-specific activation of the Gin4 kinase. J. Cell Biol. 143:709-717.
9. Chant, J., M. Mischke, E. Mitchell, I. Herskowitz, and J.R. Pringle. 1995. Role of Bud3p in producing the axial budding pattern of yeast. J. Cell Biol. 129: 767-778.
10. Cooper, J.A., and O.P. Kiehart. 1996. Septins may form a ubiquitous family of cytoskeletal filaments. J. Cell Biol. 134:1345-1348.
11. De Virgilio, C., D.J. DeMarini, and J.R. Pringle. 1996. SPR28, a sixth member of the septin gene family in Saccharomyces cerevisiae that is expressed specifically in sporulating cells. Microbiology. 142:2897-2905.
12. DeMarini, D.J., A.E.M. Adams, H. Fares, C. De Virgilio, G. Valle, J.S. Chuang, and J.R. Pringle. 1997. A septin-based hierarchy of proteins required for localized deposition of chitin in the Saccharomyces cerevisiae cell wall. J. Cell Biol. 139:75-93.
13. Fares, H., L. Goetsch, and J.R. Pringle. 1996. Identification of a developmentally regulated septin and involvement of the septins in spore formation in S. cerevisiae. J. Cell Biol. 132:399-411.
14. Fares, H., M. Peifer, and J.R. Pringle. 1995. Localization and possible functions of Drosophila septins. Mol. Biol. Cell. 12:1843-1859.
15. Field, C.M., O.S. Al-Awar, J. Rosenblatt, M.L. Wong, and B. Alberts. 1996. A purified Drosophila septin complex forms filaments and exhibits GTPase activity. J. Cell Biol. 133:605-616.
16. Flescher, E.G., K. Madden, and M, Snyder. 1993. Components required for cytokinesis are important for bud site selection in yeast. J. Cell Biol. 122:373-386.
17. Ford, S.K., and J.R. Pringle. 1991. Cellular morphogenesis in the Saccharomyces cerevisiae cell cycle: localization of the CDC11 gene product and the timing of events at the budding site. Dev. Genet. 12:281-292.
18. Fuchs, E., and D.W. Cleveland. 1998. A structural scaffolding of intermediate filaments in health and disease. Science. 279:514-519.
19. Gietz, R.D., and A. Sugino. 1988. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene. 74:527-534.
20. Guthrie, C., and G.R. Fink. 1991. Guide to Yeast Genetics and Molecular Biology. Methods Enzymol. 194:1-93.
21. Haarer, B.K., and J.R. Pringle. 1987. Immunofluorescence localization of the Saccharomyces cerevisiae CDC12 gene product to the vicinity of the 10-nm filaments in the mother-bud neck. Mol. Cell Biol. 7:3678-3687.
22. Hartwell, L.H. 1971. Genetic control of the cell division cycle in yeast. IV. Genes controlling bud emergence and cytokinesis. Exp. Cell Res. 69:265-276.
23. Hsu, S.C., C.D. Hazuka, R. Roth, D.L. Folettie, J. Heuser, and R.H. Scheller. 1998. Subunit composition, protein interactions, and structures of the mammalian brain sec6/8 complex and septin filaments. Neuron. 20:1111-1122.
24. Jones, J., and L. Prakash. 1990. Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers. Yeast. 6:363-366.
25. Kato, K. 1990. A collection of cDNA clones with specific expression patterns in the mouse brain. Eur. J. Neurosci. 2:704-711.
26. Kellogg, D.R., T. Kikuchi, T. Fujii-Nakata, C.W. Turck, and A.W. Murray. 1995. Members of the NAP/SET family of proteins interact specifically with B-type cyclins. J. Cell Biol. 130:661-673.
27. Kim, H.B., B.K. Haarer, and J.R. Pringle. 1991. Cellular morphogenesis in the Saccharomyces cerevisiae cell cycle: localization of the CDC3 gene product and the timing of events at the budding site. J. Cell Biol. 112:535-544.
28. Kinoshita, M., S. Kumar, A. Mizoguchi, C. Ide, A. Kinoshita, T. Haraguchi, Y. Hiraoka, and M. Noda. 1997. Nedd5, a mammalian septin, is a novel cyloskelelal component interacting with actin-based structures. Genes Dev. 11: 1535-1547.
29. Kumar, S., Y. Tomooka, and M. Noda. 1992. Identification of a set of genes with developmentally down-regulated expression in the mouse brain. Biochem. Biophys. Res. Commun. 185:1155-1161.
30. Lippincott, J., and R. Li. 1998. Sequential assembly of myosin II, an IQGAP-like protein, and filamentous actin to a ring structure involved in budding yeast cytokinesis. J. Cell Biol. 140:355-366.
31. Longtine, M.S., D.J. DeMarini, M.L. Valencik, O.S. Al-Awar, H. Fares, C. De Virgilio, and J.R. Pringle. 1996. The septins: roles in cytokinesis and other processes. Curr. Opin. Cell Biol. 8:106-119.
32. Longtine, M.S., H. Fares, and J.R. Pringle. 1998. Role of the yeast Gin4p protein kinase in septin assembly and the relationship between septin assembly and septin function. J. Cell Biol. 143:719-736.
33. Mitchell, D.A., T.K. Marshall, and R.J. Deschenes. 1993. Vectors for the inducible overexpression of glutathione S-transferase fusion proteins in yeast. Yeast. 9:715-723.
34. Nakatsura, S., K. Sudo, and Y. Nakamura. 1994. Molecular cloning of a novel human cDNA homologous to CDC10 in Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 202:82-87.
35. Neufeld, T.P., and G.M. Rubin. 1994. The Drosophila peanut gene is required or cytokinesis and encodes a protein similar to yeast putative bud neck filament proteins. Cell. 77:371-379.
36. Nottenburg, C., W.M. Gallatin, and T. St. John. 1990. Lymphocyte HEV adhesion variants differ in the expression of multiple gene sequences. Gene. 95: 279-284.
37. Novick, P., and D. Botstein. 1985. Phenotypic analysis of temperature-sensitive yeast actin mutants. Cell. 40:405-416.
38. Pringle, J.R., A.E. Adams, D.G. Drubin, and B.K. Haarer. 1991. Immunofluorescence methods for yeast. Methods Enzymol. 194:565-602.
39. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboatory Manual. 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
40. Sanders, S.L., and C.M. Field. 1995. Bud-site selection is only skin deep. Curr. Biol. 5:1213-1215.
41. Sanders, S.L., and I. Herskowitz. 1996. The Bud4 protein of yeast, required for axial budding, is localized to the mother-bud neck in a cell cycle-dependent manner. J. Cell Biol. 134:413-427.
42. Sawin, K.E., T.J. Mitchison, and L.G. Wordeman. 1992. Evidence for kinesin-related proteins in the mitotic apparatus using peptide antibodies. J. Cell Sci. 101:303-313.
43. Siegel, L., and K. Monty. 1966. Determination of molecular weights and frictional ratios of proteins in impure systems by use of gel filtration and density gradient centrifugation. Application to crude preparations of sulfite and hydroxylamine reductases. Biochim. Biophys. Acta. 112:346-362.
44. Sikorski, R.S., and P. Hieter. 1989. A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 122:19-27.
45. Soll, D.R., and L.H. Mitchell. 1983. Filamentous ring formation in the dimorphic yeast Candida albicans. J. Cell Biol. 96:486-493.
46. Witke, W., A.V. Podtelejnikov, A. Di Nardo, J.D. Sutherland, C. Dotti, and M. Mann. 1998. In mouse brain profilin I and profilin II associate with regulators of the cndocytic pathway and actin assembly. EMBO (Eur. Miol. Biol. Organ.) J. 17:967-976.
47. Zheng, Y., M.L. Wong, B. Alberts, and T. Milchison. 1996. Nucleation of microtubule assembly by a g-tubulin containing ring complex. Nature. 378: 578-583.