The UNI1 and UNI2 genes function in the transition of triplet to doublet microtubules between the centriole and cilium in Chlamydomonas

Molecular Biology of the Cell

Volumn 20, Issue 1, 2009, Pages 368-378

  Piasecki, Brian P ^a   Silflow, Carolyn D ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GENE PRODUCT; PROTEIN UNI1; PROTEIN UNI2; UNCLASSIFIED DRUG; PROTOZOAL PROTEIN; TUBULIN;

ARTICLE; CHLAMYDOMONAS REINHARDTII; CONTROLLED STUDY; EUKARYOTIC FLAGELLUM; GENE FUNCTION; GENE INTERACTION; MICROTUBULE; NONHUMAN; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION; ANIMAL; CENTRIOLE; CYTOLOGY; GENETICS; IMMUNOHISTOCHEMISTRY; METABOLISM; ULTRASTRUCTURE;

ALGAE; CHLAMYDOMONAS; CHLAMYDOMONAS REINHARDTII; EUKARYOTA;

ANIMALS; CENTRIOLES; CHLAMYDOMONAS REINHARDTII; CILIA; IMMUNOHISTOCHEMISTRY; MICROTUBULES; PROTOZOAN PROTEINS; TUBULIN;

EID: 63049112724     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.E08-09-0900     Document Type: Article

References (49)

1. Afzelius, B. A. (2004). Cilia-related diseases. J. Pathol. 204, 470-477.
2. Albrecht-Buehler, G., and Bushnell, A. (1980). The ultrastructure of primary cilia in quiescent 3T3 cells. Exp. Cell Res. 126, 427-437.
3. Beech, P. L., Heimann, K., and Melkonian, M. (1991). Development of the flagellar apparatus during the cell cycle in unicellular algae. Protoplasma 164, 23-37.
4. Cavalier-Smith, T. (1974). Basal body and flagellar development during the vegetative cell cycle and the sexual cycle of Chlamydomonas reinhardtii. J. Cell Sci. 16, 529-556.
5. Cavalier-Smith, T. (2002). The phagotrophic origin of eukaryotes and phylogenetic classification of Protozoa. Int. J. Syst. Evol. Microbiol. 52, 297-354.
6. Christensen, S. T., Pedersen, L. B., Schneider, L., and Satir, P. (2007). Sensory cilia and integration of signal transduction in human health and disease. Traffic 2, 97-109.
7. Cole, D. G. (2003). The intraflagellar transport machinery of Chlamydomonas reinhardtii. Traffic 4, 435-442.
8. Cole, D. G., Diener, D. R., Himelblau, A. L., Beech, P. L., Fuster, J. C., and Rosenbaum, J. L. (1998). Chlamydomonas kinesin-II-dependent intraflagellar transport (IFT): IFT particles contain proteins required for ciliary assembly in Caenorhabditis elegans sensory neurons. J. Cell Biol. 141, 993-1008.
9. Dammermann, A., Müller-Reichert, T., Pelletier, L., Habermann, B., Desai, A., and Oegema, K. (2004). Centriole assembly requires both centriolar and percentriolar material proteins. Dev. Cell 7, 815-829.
10. Deane, J. A., Cole, D. G., Seeley, E. S., Diener, D. R., and Rosenbaum, J. L. (2001). Localization of intraflagellar transport protein IFT52 identifies basal body transitional fibers as the docking site for IFT particles. Curr. Biol. 11, 1586-1590.
11. Dutcher, S. K., Morrissette, N. S., Preble, A. M., Rackley, C., and Stanga, J. (2002). ε-tubulin is an essential component of the centriole. Mol. Biol. Cell 13, 3859-3869.
12. Dutcher, S. K., and Trabuco, E. C. (1998). The UNI3 gene is required for assembly of basal bodies of Chlamydomonas and encodes δ-tubulin, a new member of the tubulin superfamily. Mol. Biol. Cell 9, 1293-1308.
13. Fromherz, S., Giddings, T. H., Jr., Gomex-Ospina, N., and Dutcher, S. K. (2004). Mutations in α-tubulin promote basal body maturation and flagellar assembly in the absence of α-tubulin. J. Cell Sci. 117, 303-314.
14. Gaffal, K. P. (1988). The basal body-root complex of Chlamydomonas reinhardtii during mitosis. Protoplasma 143, 139-148.
15. Geimer, S., and Melkonian, M. (2004). The ultrastructure of the Chlamydomonas reinhardtii basal apparatus: identification of an early marker of radial asymmetry inherent in the basal body. J. Cell Sci. 117, 2663-2674.
16. Gilula, N. B., and Satir, P. (1972). The ciliary necklace. A ciliary membrane specialization. J. Cell Biol. 53, 494-509.
17. Gould, R. R. (1975). The basal bodies of Chlamydomonas reinhardtii. Formation from probasal bodies, isolation, and partial characterization. J. Cell Biol. 65, 65-74.
18. Holmes, J. A., and Dutcher, S. K. (1989). Cellular asymmetry in Chlamydomonas reinhardtii. J. Cell Sci. 94, 273-285.
19. Hoops, H. J., and Witman, G. B. (1983). Outer doublet heterogeneity reveals structural polarity related to beat direction in Chlamydomonas flagella. J. Cell Biol. 97, 902-908.
20. Huang, B., Ramanis, Z., Dutcher, S. K., and Luck, D. J. (1982). Uniflagellar mutants of Chlamydomonas: evidence for the role of basal bodies in transmission of positional information. Cell 29, 745-753.
21. Kathir, P., LaVoie, M., Brazelton, W. J., Haas, N. A., Lefebvre, P. A., and Silflow, C. D. (2003). Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot. Cell 2, 362-379.
22. Kindle, K. L. (1990). High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA 87, 1228-1232.
23. Kozminski, K. G., Johnson, K. A., Forscher, P., and Rosenbaum, J. L. (1993). A motility in the eukaryotic flagellum unrelated to flagellar beating. Proc. Natl. Acad. Sci. USA 90, 5519-5523.
24. Leidel, S., Delattre, M., Cerutti, L., Baumer, K., and Gonczy, P. (2005). SAS-6 defines a protein family required for centrosome duplication in C. elegans and in human cells. Nat. Cell Biol. 7, 115-125.
25. Levine, R. P., and Ebersold, W. T. (1960). The genetics and cytology of Chlamydomonas. Annu. Rev. Microbiol. 14, 197-216.
26. Linck, R. W., and Stephens, R. E. (2007). Functional protofilament numbering of ciliary, flagellar, and centriolar microtubules. Cell Motil. Cytoskelet. 64, 489-495.
27. Marshall, W. F. (2008). The cell biological basis of ciliary disease. J. Cell Biol. 180, 17-21.
28. Matsuura, K., Lefebvre, P. A., Kamiya, R., and Hirono, M. (2004). Bld10p, a novel protein essential for basal body assembly in Chlamydomonas: localization to the cartwheel, the first ninefold symmetrical structure appearing during assembly. J. Cell Biol. 165, 663-671.
29. Merchant, S. S. et al. (2007). The Chlamydomonas genome reveals the evolution of key animal and plant functions. Science 318, 245-250.
30. Nakazawa, Y., Hiraki, M., Kamiya, R., and Hirono, M. (2007). SAS-6 is a cartwheel protein that establishes the 9-fold symmetry of the centriole. Curr. Biol. 17, 2169-2174.
31. O'Toole, E. T., Giddings, T. H., McIntosh, J. R., and Dutcher, S. K. (2003). Three-dimensional organization of basal bodies from wild-type and δ-tubulin deletion strains of Chlamydomonas reinhardtii. Mol. Biol. Cell 14, 2999-3012.
32. Paintrand, M., Moudjou, M., Delacroix, H., and Bornens, M. (1992). Centrosome organization and centriole architecture: their sensitivity to divalent cations. J. Struct. Biol. 108, 107-128.
33. Pazour, G. J., Dickert, B. L., and Witman, G. B. (1999). The DHC1b (DHC2) isoform of cytoplasmic dynein is required for flagellar assembly. J. Cell Biol. 144, 473-481.
34. Pedersen, L. B., Veland, I. R., Schroder, J. M., and Christensen, S. T. (2008). Assembly of primary cilia. Dev. Dyn. 237, 1993-2006.
35. Piasecki, B. P., Lavoie, M., Tam, L. W., Lefebvre, P. A., and Silflow, C. D. (2008). The Uni2 phosphoprotein is a cell cycle-regulated component of the basal body maturation pathway in Chlamydomonas reinhardtii. Mol. Biol. Cell 19, 262-273.
36. Ringo, D. L. (1967). Flagellar motion and fine structure of the flagellar apparatus in Chlamydomonas. J. Cell Biol. 33, 543-571.
37. Rosenbaum, J. L., and Witman, G. B. (2002). Intraflagellar transport. Nat. Rev. Mol. Cell Biol. 3, 813-825.
38. Sager, R., and Granick, S. (1953). Nutritional studies with Chlamydomonas reinhardtii. Ann. NY Acad. Sci. 56, 831-838.
39. Sanders, M. A., and Salisbury, J. L. (1995). Immunofluorescence microscopy of cilia and flagella. Methods Cell Biol. 47, 163-169.
40. Sato, T. (1968). A modified method for lead staining of thin sections. J. Electron Microsc. 17, 158-159.
41. Scholey, J. M. (2008). Intraflagellar transport motors in cilia: moving along the cell's antenna. J. Cell Biol. 180, 23-29.
42. Silflow, C. D., LaVoie, M., Tam, L. W., Tousey, S., Sanders, M., Wu, W., Borodovsky, M., and Lefebvre, P. A. (2001). The Vfl1 Protein in Chlamydomonas localizes in a rotationally asymmetric pattern at the distal ends of the basal bodies. J. Cell Biol. 153, 63-74.
43. Sizova, I., Fuhrmann, M., and Hegemann, P. (2001). A streptomyces rimosus aph VIII gene coding for a new type of phosphotransferase provides stable antibiotic resistance to Chlamydomonas reinhardtii. Gene 277, 221-229.
44. Tam, L. W., and Lefebvre, P. A. (1993). Cloning of flagellar genes in Chlamydomonas reinhardtii by DNA insertional mutagenesis. Genetics 135, 375-384.
45. Vashishtha, M., Walther, Z., and Hall, J. L. (1996). The kinesin-homologous protein encoded by the Chlamydomonas FLA10 gene is associated with basal bodies and centrioles. J. Cell Sci. 109, 541-549.
46. Vorobjev, I. A., and Chentsov, Y. S. (1980). The ultrastructure of centriole in mammalian tissue culture cells. Cell Biol. Int. Rep. 4, 1037-1044.
47. Vorobjev, I. A., and Chentsov, Y. S. (1982). Centrioles in the cell cycle. I. Epithelial cells. J. Cell Biol. 93, 938-949.
48. Weiss, R. L. (1984). Ultrastructure of the flagellar roots in Chlamydomonas gametes. J. Cell Sci. 67, 133-143.
49. Weiss, R. L., Goodenough, D. A., and Goodenough, U. W. (1977). Membrane particle arrays associated with the basal body and with contractile vacuole secretion in Chlamydomonas. J. Cell Biol. 72, 133-143.