Bld10p, a novel protein essential for basal body assembly in Chlamydomonas: Localization to the cartwheel, the first ninefold symmetrical structure appearing during assembly

Journal of Cell Biology

Volumn 165, Issue 5, 2004, Pages 663-671

  Matsuura, Kumi ^a,d   Lefebvre, Paul A ^b   Kamiya, Ritsu ^a,c   Hirono, Masafumi ^a  

^a UNIVERSITY OF TOKYO   (Japan)

^b UNIVERSITY OF MINNESOTA   (United States)

^c JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^d RIKEN   (Japan)

Author keywords

Centriole; Centrosome; Coiled coil; Flagella; Mitotic spindle apparatus

Indexed keywords

PROTEIN; PROTEIN BLD10P; UNCLASSIFIED DRUG;

ARTICLE; CELL DIVISION; CELL GROWTH; CENTRIOLE; CHLAMYDOMONAS REINHARDTII; ELECTRON MICROSCOPY; GENE MUTATION; IMMUNOELECTRON MICROSCOPY; KINETOSOME; MICROTUBULE ASSEMBLY; MITOSIS SPINDLE; MOLECULAR CLONING; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN LOCALIZATION; PROTEIN STRUCTURE;

ALGAL PROTEINS; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CELLS, CULTURED; CENTRIOLES; CHLAMYDOMONAS REINHARDTII; DNA, COMPLEMENTARY; FLAGELLA; IMMUNOHISTOCHEMISTRY; MICROSCOPY, ELECTRON; MICROTUBULES; MITOTIC SPINDLE APPARATUS; MODELS, ANIMAL; MOLECULAR SEQUENCE DATA; ORGANELLES; PROTOZOAN PROTEINS;

ALGAE; CHLAMYDOMONAS; CHLAMYDOMONAS REINHARDTII; EUKARYOTA; PROTOZOA;

EID: 2942633899     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.200402022     Document Type: Article

References (46)

1. Allen, R.D. 1969. The morphogenesis of basal bodies and accessory structures of the cortex of the ciliated protozoan Tetrahymena pyriformis. J. Cell Biol. 40: 716-733.
2. Alvey, P.L. 1986. Do adult centrioles contain cartwheels and lie at right angles to each other? Cell Biol. Int. Rep. 10:589-598.
3. Anderson, R.G.W., and R.M. Brenner. 1971. The formation of basal bodies (centrioles) in the rhesus monkey oviduct. J. Cell Biol. 50:10-34.
4. Cavalier-Smith, T. 1974. Basal body and flagellar development during the vegetative cell cycle and the sexual cycle of Chlamydomonas reinhardtii. J. Cell Sci. 16:529-556.
5. Compton, D.A. 1998. Focusing on spindle poles. J. Cell. Sci. 111:1477-1481.
6. Debuchy, R., S. Purton, and J.-D. Rochaix. 1989. The argininosuccinate lyase gene of Chlamydomonas reinhardtii: an important tool for nuclear transformation and for correlating the genetic and molecular maps of the ARG7 locus. EMBO J. 8:2803-2809.
7. Dippel, R.V. 1968. The development of basal bodies in Paramecium. Proc. Natl. Acad. Sci. USA. 61:461-468.
8. Dutcher, S.K. 2003. Elucidation of basal body and centriole functions in Chlamydomonas reinhardtii. Traffic. 4:443-451.
9. Dutcher, S.K., and E.C. Trabuco. 1998. The UNI3 gene is required for assembly of basal bodies of Chlamydomonas and encodes δ-tubulin, a new member of the tubulin superfamily. Mol. Biol. Cell. 9:1293-1308.
10. Dutcher, S.K., N.S. Morrissette, A.M. Preble, C. Rackley, and J. Stanga. 2002. ε-tubulin is an essential component of the centriole. Mol. Biol. Cell. 13: 3859-3869.
11. Ehler, L.L., J.A. Holmes, and S.K. Dutcher. 1995. Loss of spatial control of the mitotic spindle apparatus in a Chlamydomonas reinhardtii mutant strain lacking basal bodies. Genetics. 141:945-960.
12. Fernandez, E., R. Schnell, L.P. Ranum, S.C. Hussey, C.D. Silflow, and P.A. Lefebvre. 1989. Isolation and characterization of the nitrate reductase structural gene of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA. 86:6449-6453.
13. Gaffal, K.P. 1988. The basal body-root complex of Chlamydomonas reinhardtii during mitosis. Protoplasma. 143:118-129.
14. Gavin, R.H. 1984. In vitro reassembly of basal body components. J. Cell Sci. 66: 147-154.
15. Gibbons, I.R., and A.V. Grimstone. 1960. On flagellar structure in certain flagellates. J. Biophys. Biochem. Cytol. 7:697-716.
16. Goodenough, U.W., and H.S. St. Clair. 1975. BALD-2: a mutation affecting the formation of doublet and triplet sets of microtubules in Chlamydomonas reinhardtii. J. Cell Biol. 66:480-491.
17. Gorman, D.S., and R.P. Levine. 1965. Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA. 54:1665-1669.
18. Harris, E.H. 1989. The Chlamydomonas Sourcebook. Academic Press, San Diego, CA. 780 pp.
19. Heald, R., R. Tournebize, A. Habermann, E. Karsenti, and A. Hyman. 1997. Spindle assembly in Xenopus egg extracts: respective roles of centrosomes and microtubule self-organization. J. Cell Biol. 138:615-628.
20. 2+-binding protein. J. Cell Biol. 107:121-131.
21. Holmes, J.A., and S.K. Dutcher. 1989. Cellular asymmetry in Chlamydomonas reinhardtii. J. Cell Sci. 94:273-285.
22. Kallenbach, R.J. 1982. Origin and maturation of centrioles in association with the nuclear envelope in hypertonic-stressed sea urchin eggs. Eur. J. Cell Biol. 28: 68-76.
23. Kathir, P., M. LaVoie, W.J. Brazelton, N.A. Haas, P.A. Lefebvre, and C.D. Silflow. 2003. Molecular map of the Chlamydomonas reinhardtii nuclear genome. Eukaryot. Cell 2:362-379.
24. Kindle, K.L. 1990. High-frequency nuclear transformation of Chlamydomonas reinhardtii. Proc. Natl. Acad. Sci. USA. 87:1228-1232.
25. Khodjakov, A., R.W. Cole, B.R. Oakley, and C.L. Rieder. 2000. Centrosome-independent mitotic spindle formation in vertebrates. Curr. Biol. 10:59-67.
26. Kulp, D., D. Hausseler, M.G. Reese, and F.H. Eekman. 1996. A generalized hidden Markov model for the recognition of human genes in DNA. Proc. Int. Conf. Intell. Syst. Mol. Biol. 4:134-142.
27. Lukashin, A.V., and M. Borodovsky. 1998. Gene Mark.hmm: new solutions for gene finding. Nucleic Acids Res. 26:1107-1115.
28. Lupas, A., M. Van Dyke, and J. Stock. 1991. Predicting coiled coils from protein sequences. Science. 252:1162-1164.
29. Maniotis, A., and M. Schliwa. 1991. Microsurgical removal of centrosomes blocks cell reproduction and centriole generation in BSC-1 cells. Cell. 67:495-504.
30. Matsuura, K., P.A. Lefebvre, R. Kamiya, and M. Hirono. 2002. Kinesin-II is not essential for mitosis and cell growth in Chlamydomonas. Cell Motil. Cytoskeleton. 52:195-201.
31. Olmsted, J.B. 1981. Affinity purification of antibodies from diazotized paper bots of heterogeneous protein samples. J. Biol. Chem. 256:11955-11957.
32. Preble, A.M., T.H. Giddings Jr., and S.K. Dutcher. 2001. Extragenic bypass suppressors of mutations in the essential gene BLD2 promote assembly of basal bodies with abnormal microtubules in Chlamydomonas reinhardtii. Genetics. 157:163-181.
33. Rieder, C.L., S. Faruki, and A. Khodjakov. 2001. The centrosome in vertebrates: more than a microtubule-organizing center. Trends Cell Biol. 11:413-419.
34. Ruiz, F., J. Beisson, J. Rossier, and P. Dupuis-Williams. 1999. Basal body duplication in Paramecium requires γ-tubulin. Curr. Biol. 9:43-46.
35. Ruiz, F., A. Krzywicka, C. Klotz, A. Keller, J. Cohen, F. Koll, G. Balavonie, and J. Beisson. 2000. The SM19 gene, required for duplication of basal bodies in Paramecium, encodes a novel tubulin, η-tubulin. Curr. Biol. 10:1451-1454.
36. Sager, R., and S. Granick. 1953. Nutritional studies with Chlamydomonas reinhardtii. Ann. NY Acad. Sci. 56:831-838.
37. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Extraction of RNA with guanidinium thiocyanate followed by centrifugation in cesium chloride solutions. In Molecular Cloning: A Laboratory Manual. Second edition. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 7.19-7.22.
38. Shimogawara, K., S. Fujiwara, A. Grossman, and H. Usuda. 1998. High-efficiency transformation of Chlamydomonas reinhardtii by electroporation. Genetics. 148:1821-1828.
39. Silflow, C.D., M. La Voie, L.-W. Tam, S. Tousey, M. Sanders, W.-C. Wu, M. Borodovsky, and P.A. Lefebvre. 2001. The Vfl1 protein in Chlamydomonas localizes in a rotationally asymmetric pattern at the distal ends of the basal bodies. J. Cell Biol. 153:63-74.
40. Smith, E.F., and P.A. Lefebvre. 1996. PF16 encodes a protein with armadillo repeats and localizes to a single microtubule of the central apparatus in Chlamydomonas flagella. J. Cell Biol. 132:359-370.
41. Stubblefield, E., and B.R. Brinkley. 1967. Architecture and function of the mammalian centriole. In Formation and Fate of Cell Organelles. K.B. Warren, editor. Academic Press, Inc., New York. 175-218,
42. Taillon, B.E., S.A. Adler, J.P. Suhan, and J.W. Jarvik. 1992. Mutational analysis of centrin: An EF-hand protein associated with three distinct contractile fibers in the basal body apparatus of Chlamydomonas. J. Cell Biol. 119:1613-1624.
43. Tam, L.-W., and P.A. Lefebvre. 1993. Cloning of flagellar genes in Chlamydomonas reinhardtii by DNA insertional mutagenesis. Genetics. 135:375-384.
44. Tamm, S., and S.L. Tamm. 1980. Origin and development of free kinetosomes in the flagellates Deltotrichonympha and Koruga. J. Cell Sci. 42:189-205.
45. Wright, R.L., B. Chojnacki, and J.W. Jarvik. 1983. Abnormal basal-body number, location, and orientation in a striated fiber-defective mutant of Chlamydomonas reinhardtii. J. Cell Biol. 96:1697-1707.
46. Wright, R.L., J. Salisbury, and J.W. Jarvik. 1985. A nucleus-basal body connector in Chlamydomonas reinhardtii that may function in basal body localization or segregation. J. Cell Biol. 101:1903-1912.