DNA context and promoter activity affect gene expression in lentiviral vectors

Acta Biomedica de l'Ateneo Parmense

Volumn 79, Issue 3, 2008, Pages 192-196

  Mao, Gensheng ^a   Marotta, Francesco ^b   Yu, Jia ^c   Zhou, Liang ^c   Yu, Yan ^c   Wang, Lina ^c   Chui, Dehua ^c  

^a GENERAL HOSPITAL OF CHINESE PEOPLE'S ARMED POLICE FORCES   (China)

^b Nutraceutical Nutragenomic Unit   (Italy)

^c PEKING UNIVERSITY   (China)

Author keywords

DNA context; Elongation factor 1 alpha; Promoter activity

Indexed keywords

DNA; ELONGATION FACTOR 1ALPHA; GREEN FLUORESCENT PROTEIN; LENTIVIRUS VECTOR; VIRUS DNA;

ARTICLE; CELL LINE; CELL TYPE; CONTROLLED STUDY; CYTOMEGALOVIRUS; GENE DELIVERY SYSTEM; GENE EXPRESSION; GENE OVEREXPRESSION; GENOMICS; HELA CELL; HUMAN; HUMAN CELL; LENTIVIRINAE; NONHUMAN; PROMOTER REGION; REPORTER GENE; TRANSGENE; VIRUS GENOME; VIRUS TITRATION; COMPARATIVE STUDY; GENE VECTOR; GENETIC TRANSDUCTION; GENETICS; POLYMERASE CHAIN REACTION;

CELL LINE; DNA, VIRAL; GENE EXPRESSION; GENETIC VECTORS; HUMANS; LENTIVIRUS; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC; TRANSDUCTION, GENETIC;

EID: 63049095456     PISSN: 03924203     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. Miyoshi H, Blomer U, Takahashi M, Gage FH, Verma IM. Development of a self-inactivating lentivirus vector. J Virol 1998; 72: 8150-8157 (Pubitemid 28421810)
2. Naldini L, Blomer U, Gage FH, Trono D, Verma IM. Efficient transfer, integration, and sustained long-term expression of the transgene in adult rat brains injected with a lentiviral vector. Proc Natl Acad Sci USA 1996; 93: 11382-11388 (Pubitemid 26347130)
3. Naldini L, Blomer U, Gallay P, et al. In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 1996; I272: 263-267
4. Cherry SR, Biniszkiewicz, D, van Parijs L, Baltimore D, Jaenisch R. Retroviral expression in embryonic stem cells and hematopoietic stem cells. Mol Cell Biol 2000; 20: 7419-7426
5. Ikawa M, Tanaka N, Kao WW, Verma IM. Generation of transgenic mice using lentiviral vectors: a novel preclinical assessment of lentiviral vectors for gene therapy. Mol Ther 2003; 8: 666-673 (Pubitemid 37321696)
6. Pfeifer A, Ikawa M, Dayn Y, Verma IM. Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos. Proc Natl Acad Sci USA 2002; 99: 2140-2145 (Pubitemid 34169028)
7. Dull T, Zufferey R, Kelly M, et al. A third-generation lentivirus vector with a conditional packaging system. J Virol 1998; 72: 8463-8471 (Pubitemid 28471781)
8. Zufferey R, Donello JE, Trono D, Hope TJ. Woodchuck hepatitis virus posttranscriptional regulatory element enhances expression of transgenes delivered by retroviral vectors. J Virol 1999; 73: 2886-2892 (Pubitemid 29135675)
9. Butler SL, Hansen MS, Bushman FD. A quantitative assay for HIV DNA integration in vivo. Nat Med 2001; 7: 631-634 (Pubitemid 32448333)
10. Park F, Kay MA. Modified HIV-1 based lentiviral vectors have an effect on viral transduction efficiency and gene expression in vitro and in vivo. Mol Ther 2001; 4: 164-173 (Pubitemid 32851279)
11. Sastry L, Johnson T, Hobson MJ, Smucker B, Cornetta, K. Titering lentiviral vectors: comparison of DNA, RNA and marker expression methods. Gene Ther 2002; 9: 1155-1162