The size and ratio of homologous sequence to non-homologous sequence in gene disruption cassette influences the gene targeting efficiency in Beauveria bassiana

Applied Microbiology and Biotechnology

Volumn 82, Issue 5, 2009, Pages 891-898

  Ma, Jin Cheng ^a   Zhou, Qun ^a   Zhou, Yong Hong ^a   Liao, Xing Gang ^a   Zhang, Yong Jun ^a   Jin, Dan ^a   Pei, Yan ^a  

^a SOUTHWEST UNIVERSITY   (China)

Author keywords

Beauveria bassiana; Herpes simplex virus thymidine kinase; Homologous recombination frequency; Ratio of homologous sequence to non homologous sequence

Indexed keywords

BEAUVERIA BASSIANA; CONVENTIONAL APPROACHES; ENTOMOPATHOGENIC FUNGUS; GENE DISRUPTIONS; GENE FUNCTIONS; GENE REPLACEMENTS; GENE TARGETING; HERPES SIMPLEX VIRUS THYMIDINE KINASE; HOMOLOGOUS RECOMBINATION FREQUENCY; RATIO OF HOMOLOGOUS SEQUENCE TO NON-HOMOLOGOUS SEQUENCE; SELECTABLE MARKERS; TARGET GENES; TRANSFORMANTS;

GENETIC ENGINEERING;

VIRUSES;

FUNGUS; GENE EXPRESSION; GENETIC MARKER; HOMOLOGY; RECOMBINATION;

BEAUVERIA; BLOTTING, SOUTHERN; DNA, FUNGAL; GENE TARGETING; GENES, FUNGAL; MUTAGENESIS, INSERTIONAL; POLYMERASE CHAIN REACTION; RECOMBINATION, GENETIC; SEQUENCE HOMOLOGY, NUCLEIC ACID; THYMIDINE KINASE; VIRAL PROTEINS;

CORDYCEPS BASSIANA; FUNGI; SIMPLEXVIRUS;

EID: 62949163461     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1844-0     Document Type: Article

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