Can intrabodies targeted to the secretory compartment interact with a cytosolic protein? A comment on the article by Sawahata et al. "Cytoplasmic expression and specific binding of the VH/VL single domain intrabodies in transfected NIH3T3 cells", Exp. Mol. Pathol. 2008 Nov 27; Epub ahead of print

Experimental and Molecular Pathology

Volumn 86, Issue 2, 2009, Pages 138-

  Cardinale, Alessio ^a   Biocca, Silvia ^b  

^a IRCCS SAN RAFFAELE PISANA   (Italy)

^b UNIVERSITY OF ROME TOR VERGATA   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEASOME; SIGNAL PEPTIDE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; VON HIPPEL LINDAU PROTEIN;

CELL STRAIN 3T3; CYTOPLASM; CYTOSOL; ENDOPLASMIC RETICULUM; GENETIC TRANSFECTION; IMMUNOPRECIPITATION; LETTER; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN INTERACTION;

ANIMALS; ANTIBODIES; CELL COMPARTMENTATION; CYTOSOL; ENDOPLASMIC RETICULUM; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; IMMUNOGLOBULIN VARIABLE REGION; MICE; NIH 3T3 CELLS; PROTEIN BINDING; TRANSFECTION;

EID: 62949140505     PISSN: 00144800     EISSN: 10960945     Source Type: Journal    
DOI: 10.1016/j.yexmp.2009.01.002     Document Type: Letter

References (5)

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2. Drisaldi B., Stewart R.S., Adles C., Stewart L.R., Quaglio E., Biasini E., Fioriti L., Chiesa R., and Harris D.A. Mutant PrP is delayed in its exit from the endoplasmic reticulum, but neither wild-type nor mutant PrP undergoes retrotranslocation prior to proteasomal degradation. J. Biol. Chem. 278 (2003) 21732-21743
3. Sc formation. J. Neurochem. 101 (2007) 1516-1526
4. Sawahata, R., Sato, M., Kitani, H., 2008. Cytoplasmic expression and specific binding of the V(H)/V(L) single domain intrabodies in transfected NIH3T3 cells. Exp Mol Pathol. Nov 27. [Electronic publication ahead of print]
5. Yu H., Kaung G., Kobayashi S., and Kopito R.R. Cytosolic degradation of T-cell receptor alpha chains by the proteasome. J. Biol. Chem. 272 (1997) 20800-20804