Kin5 knockdown in Tetrahymena thermophila using RNAi blocks cargo transport of Gef1

PLoS ONE

Volumn 4, Issue 3, 2009, Pages

  Awan, Aashir ^a   Bell, Aaron J ^a   Satir, Peter ^a  

^a ALBERT EINSTEIN COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GUANINE NUCLEOTIDE EXCHANGE FACTOR; GUANINE NUCLEOTIDE EXCHANGE FACTOR 1; KINESIN; KINESIN 2; KINESIN 5; UNCLASSIFIED DRUG; PRIMER DNA; PROTOZOAL PROTEIN;

ARTICLE; AXONEME; CELL TRANSFORMATION; CILIARY MOTILITY; CONTROLLED STUDY; IMMUNOFLUORESCENCE TEST; IMMUNOPRECIPITATION; KNOCKOUT GENE; NONHUMAN; PROTEIN ANALYSIS; PROTEIN FUNCTION; PROTEIN TRANSPORT; RNA INTERFERENCE; SIGNAL TRANSDUCTION; TETRAHYMENA THERMOPHILA; AMINO ACID SEQUENCE; ANIMAL; CHEMISTRY; FLUORESCENT ANTIBODY TECHNIQUE; GENE SILENCING; GENETICS; METABOLISM; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROMOTER REGION; SEQUENCE HOMOLOGY;

EUKARYOTA; TETRAHYMENA; TETRAHYMENA THERMOPHILA;

AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; DNA PRIMERS; FLUORESCENT ANTIBODY TECHNIQUE; GENE KNOCKDOWN TECHNIQUES; MOLECULAR SEQUENCE DATA; PROMOTER REGIONS, GENETIC; PROTEIN TRANSPORT; PROTOZOAN PROTEINS; RNA INTERFERENCE; SEQUENCE HOMOLOGY, AMINO ACID; TETRAHYMENA THERMOPHILA;

EID: 62849085022     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0004873     Document Type: Article

References (30)

1. Linder JU, Engel P, Reimer A, Kruüger T, Plattner H, et al. (1999) Guanylyl cyclases with the topology of mammalian adenylyl cyclases and an N-terminal P-type ATPase-like domain in Paramecium, Tetrahymena and Plasmodium. Embo J 18: 4222-4232.
2. Christensen ST, Guerra CF, Awan A, Wheatley DN, Satir P (2003) Insulin receptor-like proteins in Tetrahymena thermophila ciliary membranes. Curr Biol 13: R50-R52.
3. Veland IR, Awan A, Pedersen LB, Yoder BK, Christensen ST (2009) Cilia and Signaling Pathways in Mammalian Development, Health and Disease J. Nephrology. In Press.
4. Rosenbaum JL, Witman GB (2002) Intraflagellar transport. [Review] Nat Rev Mol Cell Biol 3: 813-825.
5. Piperno G, Mead K, Henderson S (1996) Inner dynein arms but not outer dynein arms require the activity of kinesin homologue protein KHP1 (FLA10) to reach the distal part of flagella in Chlamydomonas. J Cell Biol 133: 371-379.
6. Brown JM, Marsala C, Kosoy R, Gaertig J (1999) Kinesin-II is preferentially targeted to assembling cilia and is required for ciliogenesis and normal cytokinesis in Tetrahymena. Mol Biol Cell 10: 3081-3096.
7. Awan A, Bernstein M, Hamasaki T, Satir P (2004) Cloning and characterization of Kin5, a novel Tetrahymena ciliary kinesin II. Cell Motil Cytoskeleton 58: 1-9.
8. Scholey JM (2003) Intraflagellar transport. Annu Rev Cell Dev Biol 19: 423-443.
9. Snow JJ, Ou G, Gunnarson AL, Walker MR, Zhou HM, et al. (2004) Two anterograde intraflagellar transport motors cooperate to build sensory cilia on C. elegans neurons. Nat Cell Biol 6: 1109-1113.
10. Pan X, Ou G, Civelekoglu-Scholey G, Blacque OE, Endres NF, et al. (2006) Mechanism of transport of IFT particles in C. elegans cilia by concerted action of kinesin-II and Osm-3 motors. J Cell Biol 174: 1035-1045.
11. Setou M, Nakagawa T, Seog DH, Hirokawa N (2000) Kinesin superfamily motor protein KIF17 and mLin-10 in NMDA receptor-containing vesicle transport. Science 288: 1796-1802.
12. Jenkins PM, Hurd TW, Zhang L, McEwen DP, Brown RL, et al. (2006) Ciliary targeting of olfactory CNG channels requires the CNGB1b subunit and the kinesin-2 motor protein, KIF17. Curr Biol 16: 1211-1216.
13. Cole DG (2003) The intraflagellar transport machinery of Chlamydomonas reinhardtii. Traffic 4: 435-442.
14. Pedersen LB, Veland IR, Schrøder JM, Christensen ST (2008) Assembly of primary cilia. Developmental Dynamics 237: 1993-2006.
15. Cassidy-Hanley D, Bowen J, Lee JH, Cole E, VerPlank LA, et al. (1997) Germline and somatic transformation of mating Tetrahymena thermophila by particle bombardment. Genetics 146: 135-147.
16. Gaertig J, Thatcher TH, Gu L, Gorovsky MA (1994) Electroporation-mediated replacement of a positively and negatively selectable beta-tubulin gene in Tetrahymena thermophila. Proc Natl Acad Sci USA 91: 4549-4553.
17. Orias E, Flacks M (1975) Macronuclear genetics of Tetrahymena. I. Random distribution of macronuclear genecopies in T. pyriformis, syngen 1. Genetics 79: 187-206.
18. Dondero F, Cavaletto M, Ghezzi AR, La Terza A, Banni M, Viarengo A (2004) Biochemical characterization and quantitative gene expression analysis of the multi-stress inducible metallothionein from Tetrahymena thermophila. Protist 155: 157-168.
19. Nilsson JR (1989) Tetrahymena in Cytotoxicology: with special reference to effects of heavy metals and selected drugs. Europ J Protistol 25: 2-25.
20. Nair S, Guerra C, Satir P (1999) A Sec7-related protein in Paramecium. FASEB J 13: 1249-1257.
21. Shang Y, Song X, Bowen J, Corstanje R, Gao Y, et al. (2002) A robust inducible-repressible promoter greatly facilitates gene knockouts, conditional expression, and overexpression of homologous and heterologous genes in Tetrahymena thermophila. Proc Natl Acad Sci U S A 99: 3734-3739.
22. Paddison PJ, Caudy AA, Sachidanandam R, Hannon GJ (2004) Short hairpin activated gene silencing in mammalian cells. Methods Mol Biol 265: 85-100.
23. Howard-Till RA, Yao MC (2006) Induction of gene silencing by hairpin RNA expression in Tetrahymena thermophila reveals a second small RNA pathway. Mol Cell Biol 26: 8731-8742.
24. Flannery RJ, French DA, Kleene SJ (2006) Clustering of cyclic-nucleotide-gated channels in olfactory cilia. Biophys J 91: 179-188.
25. Hong DH, Pawlyk B, Sokolov M, Strissel KJ, Yang J, et al. (2003) RPGR isoforms in photoreceptor connecting cilia and the transitional zone of motile cilia. Invest Ophthalmol Vis Sci 44: 2413-2421.
26. Panizzi JR, Jessen JR, Drummond IA, Solnica-Krezel L (2007) New functions for a vertebrate Rho guanine nucleotide exchange factor in ciliated epithelia. Development 134: 921-931.
27. Nachury MV, Loktev AV, Zhang Q, Westlake CJ, Peränen J, et al. (2007) A core complex of BBS proteins cooperates with the GTPase Rab8 to promote ciliary membrane biogenesis. Cell 129: 1201-1213.
28. Smith A, Brownawell A, Macara IG (1998) Nuclear import of Ran is mediated by the transport factor NTF2. Curr Biol 8: 1403-1406.
29. Hai B, Gaertig J, Gorovsky MA (2000) Knockout heterokaryons enable facile mutagenic analysis of essential genes in Tetrahymena. Methods Cell Biol 62: 513-531.
30. Femino AM, Fay FS, Fogarty K, Singer RH (1998) Visualization of single RNA transcripts in situ. Science 280: 585-590.