Knockout heterokaryons enable facile mutagenic analysis of essential genes in Tetrahymena

Methods in Cell Biology

Volumn , Issue 62, 2000, Pages 513-531

  Hai, Bing ^a,c   Gaertig, Jacek ^b   Gorovsky, Martin A ^a  

^a UNIVERSITY OF ROCHESTER   (United States)

^b UNIVERSITY OF GEORGIA   (United States)

^c ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL; ESSENTIAL GENE; GENE; GENE TARGETING; GENETICS; METHODOLOGY; MUTAGENESIS; REVIEW; TETRAHYMENA THERMOPHILA;

ANIMALS; GENE TARGETING; GENES, ESSENTIAL; GENES, PROTOZOAN; MUTAGENESIS; TETRAHYMENA THERMOPHILA;

EID: 0033988193     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: None     Document Type: Article

References (27)

1. Allen, S. L. (1967). Genomic exclusion: A rapid means for inducing homozygous diploid lines in Tetrahymena pyriformis, Syngen I. Science 155, 575-578.
2. Bruns, P. (1986). Genetic organization of Tetrahymena. In "The Molecular Biology of Ciliated Protozoa" (J. G. Gall, ed.), pp. 27-44. Academic Press, Orlando, FL.
3. Calzone, F. J. (1982). The regulation of protein synthesis in Tetrahymena thermophila: A quantitative analysis of the factors determining the rate of tubulin synthesis and general protein synthesis in growing, starved and deciliated cells. Ph.D. Thesis, University of Rochester, Rochester, NY.
4. Cassidy-Hanley, D., Bowen, J., Lee, J., Cole, E. S., VerPlank, L. A., Gaertig, J., Gorovsky, M. A., and Bruns, P. J. (1997). Germline and somatic transformation of mating Tetrahymena thermophila by particle bombardment. Genetics 146, 135-147.
5. Chilcoat, N. D., Melia, S. M., Haddad, A., and Turkewitz, A. P. (1996). Granule lattice protein 1 (Grl1p), an acidic, calcium-binding protein in Tetrahymena thermophila dense-core secretory granules, influences granule size, shape, content organization, and release but not protein sorting or condensation. J. Cell Biol. 135, 1775-1787.
6. Doerder, F. P., Deak, J. C., and Lief, J. H. (1992). Rate of phenotypic assortment in Tetrahymena thermophila. Dev. Genet. 13, 126-132.
7. Gaertig, J., and Gorovsky, M. A. (1994). Efficient mass transformation of Tetrahymena thermophila by electroporation of conjugants. Proc. Natl. Acad. Sci. U.S.A. 89, 9196-9200.
8. Gaertig, J., Gu, L., Hai, B., and Gorovsky, M. A. (1994). High frequency vector-mediated transformation and gene replacement in Tetrahymena. Nucleic Acids Res. 22, 5391-5398.
9. Gaertig, J., Cruz, M. A., Bowen, J., Gu, L., Pennock, D. G., and Gorovsky, M. A. (1995). Acetylation of lysine 40 in alpha-tubulin is not essential in Tetrahymena thermophila. J. Cell Biol. 129, 1301-1310.
10. Gilley, D., Lee, M. S., and Blackburn, E. H. (1995). Altering specific telomerase RNA template residues affects active site function. Genes Dev. 9, 2214-2226.
11. Hai, B., and Gorovsky, M. A. (1997). Germ-line knockout heterokaryons of an essential α-tubulin gene enable high-frequency gene replacement and a test of gene transfer from somatic to germline in Tetrahymena thermophila. Proc. Natl. Acad. Sci. U.S.A. 94, 1310-1315.
12. Kahn, R. W., Andersen, B. H., and Brunk, C. F. (1993). Transformation of Tetrahymena thermophila by microinjection of a foreign gene. Proc. Natl. Acad. Sci. U.S.A. 90, 9295-9299.
13. Kirk, K. E., Harmon, B. P., Reichardt, I. K., Sedat, J. W., and Blackburn, E. H. (1997). Block in anaphase chromosome separation caused by a telomerase template mutation. Science 275, 1478-1481.
14. Liu, X., Li, B., and Gorovsky, M. A. (1996). Essential and nonessential histone H2A variants in Tetrahymena thermophila. Mol. Cell. Biol. 16, 4305-4311.
15. Martindale, D. W., Allis, C. D., and Bruns, P. J. (1982). Conjugation in Tetrahymena thermophila. A temporal analysis of cytological stages. Exp. Cell Res. 140, 227-236.
16. Nanney, D. L. (1980). "Experimental Ciliatology." Wiley, New York.
17. Orias, E., and Bruns, P. J. (1975). Induction and isolation of mutants in Tetrahymena. Methods Cell Biol. 13, 247-282.
18. Rusconi, C. P., and Cech, T. R. (1996). The anticodon is the signal sequence for mitochondrial import of glutamine tRNA in Tetrahymena. Genes Dev. 10, 2870-2880.
19. Shen, X., Yu, L., Weir, J. W., and Gorovsky, M. A. (1995). Linker histones are not essential and affect chromatin condensation in vivo. Cell (Cambridge, Mass.) 82, 47-56.
20. Shen, X. and Gorovsky, M. A. (1996). Linker histone H1 regulates specific gene expression but not global transcription in vivo. Cell (Cambridge, Mass.) 86, 475-483.
21. Sutoh, K. (1993). A transformation vector for Dictyostelium discoideum with a new selectable marker bsr. Plasmid 30, 150-154.
22. Sweeney, R., Chen, L., and Yao, M.-C. (1994). An rRNA variable region has an evolutionarily conserved essential role despite sequence divergence. Mol. Cell. Biol. 14, 4203-4215.
23. Tondravi, M. M., and Yao, M.-C. (1986). Transformation of Tetrahymena thermophila by microinjection of ribosomal RNA genes. Proc. Natl. Acad. Sci. U.S.A. 83, 4369-4373.
24. Weindruch, R. H., and Doerder, F. P. (1975). Age-dependent micronuclear deterioration in Tetrahymena thermophila. Mech. Ageing Dev. 4, 263-280.
25. Williams, N. E., Wolfe, J., and Bleyman, L. K. (1980). Long term maintenance of Tetrahymena spp. J. Protozool. 27, 327.
26. Yao, M. C. (1996). Programmed DNA deletions in Tetrahymena: Mechanisms and implications. Trends Genet. 12, 26-30.
27. Yu, L., and Gorovsky, M. A. (1997). Constitutive expression, not a particular primary sequence, is the important feature of the H3 replacement variant hv2 in Tetrahymena thermophila. Mol. Cell. Biol. 17, 6303-6310.