Cross-complementation of Clostridium perfringens PLC and Clostridium septicum α-toxin mutants reveals PLC is sufficient to mediate gas gangrene

Microbes and Infection

Volumn 11, Issue 3, 2009, Pages 413-418

  Kennedy, Catherine L ^a   Lyras, Dena ^a   Cheung, Jackie K ^a   Hiscox, Thomas J ^a   Emmins, John J ^a   Rood, Julian I ^a  

^a MONASH UNIVERSITY   (Australia)

Author keywords

Toxin; Myonecrosis; Pathogenesis; Perfringens; Septicum

Indexed keywords

ALPHA TOXIN;

ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; BACTERIAL VIRULENCE; CLOSTRIDIUM PERFRINGENS; CLOSTRIDIUM SEPTICUM; FEMALE; GAS GANGRENE; GENETIC COMPLEMENTATION; LEUKOSTASIS; MOUSE; MUTANT; NONHUMAN; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN EXPRESSION;

ANIMALS; BACTERIAL TOXINS; CALCIUM-BINDING PROTEINS; CLOSTRIDIUM PERFRINGENS; CLOSTRIDIUM SEPTICUM; FEMALE; GAS GANGRENE; GENETIC COMPLEMENTATION TEST; MICE; MICE, INBRED BALB C; TYPE C PHOSPHOLIPASES;

CLOSTRIDIUM PERFRINGENS; CLOSTRIDIUM SEPTICUM;

EID: 62649164648     PISSN: 12864579     EISSN: 1769714X     Source Type: Journal    
DOI: 10.1016/j.micinf.2009.01.002     Document Type: Article

References (20)

1. Stevens D.L. Necrotizing clostridial soft tissue infections. In: Rood J.I., McClane B.A., Songer J.G., and Titball R.W. (Eds). The Clostridia: Molecular Biology and Pathogenesis (1997), Academic Press Limited, London 144-147
2. Ellemor D.M., Baird R., Awad M.M., Boyd R.L., Rood J.I., and Emmins J.J. Use of genetically manipulated strains of Clostridium perfringens reveals that both alpha-toxin and theta-toxin are required for vascular leukostasis to occur in experimental gas gangrene. Infect. Immun. 67 (1999) 4902-4907
3. Stevens D.L., Tweten R.K., Awad M.M., Rood J.I., and Bryant A.E. Clostridial gas gangrene: evidence that alpha and theta toxins differentially modulate the immune response and induce acute tissue necrosis. J. Infect. Dis. 176 (1997) 189-195
4. Kennedy C.L., Krejany E.O., Young L.F., O'Connor J.R., Awad M.M., Boyd R.L., Emmins J.J., Lyras D., and Rood J.I. The alpha-toxin of Clostridium septicum is essential for virulence. Mol. Microbiol. 57 (2005) 1357-1366
5. Titball R.W., Naylor C.E., and Basak A.K. The Clostridium perfringens alpha-toxin. Anaerobe 5 (1999) 51-64
6. Bryant A.E., Chen R.Y., Nagata Y., Wang Y., Lee C.H., Finegold S., Guth P.H., and Stevens D.L. Clostridial gas gangrene. II. Phospholipase C-induced activation of platelet gpIIbIIIa mediates vascular occlusion and myonecrosis in Clostridium perfringens gas gangrene. J. Infect. Dis. 182 (2000) 808-815
7. Ballard J., Sokolov Y., Yuan W.L., Kagan B.L., and Tweten R.K. Activation and mechanism of Clostridium septicum alpha toxin. Mol. Microbiol. 10 (1993) 627-634
8. Gordon V.M., Nelson K.L., Buckley J.T., Stevens V.L., Tweten R.K., Elwood P.C., and Leppla S.H. Clostridium septicum alpha-toxin uses glycosylphosphatidylinositol-anchored protein receptors. J. Biol. Chem. 274 (1999) 27274-27280
9. Melton J.A., Parker M.W., Rossjohn J., Buckley J.T., and Tweten R.K. The identification and structure of the membrane-spanning domain of the Clostridium septicum alpha toxin. J. Biol. Chem. 279 (2004) 14315-14322
10. Hang'ombe M.B., Mukamoto M., Kohda T., Sugimoto N., and Kozaki S. Cytotoxicity of Clostridium septicum alpha-toxin: its oligomerization in detergent resistant membranes of mammalian cells. Microb. Pathog. 37 (2004) 279-286
11. Wichroski M.J., Melton J.A., Donahue C.G., Tweten R.K., and Ward G.E. Clostridium septicum alpha-toxin is active against the parasitic protozoan Toxoplasma gondii and targets members of the SAG family of glycosylphosphatidylinositol-anchored surface proteins. Infect. Immun. 70 (2002) 4353-4361
12. Awad M.M., Bryant A.E., Stevens D.L., and Rood J.I. Virulence studies on chromosomal alpha-toxin and theta-toxin mutants constructed by allelic exchange provide genetic evidence for the essential role of alpha-toxin in Clostridium perfringens-mediated gas gangrene. Mol. Microbiol. 15 (1995) 191-202
13. Awad M.M., Ellemor D.M., Boyd R.L., Emmins J.J., and Rood J.I. Synergistic effects of alpha-toxin and perfringolysin O in Clostridium perfringens-mediated gas gangrene. Infect. Immun. 69 (2001) 7904-7910
14. Lyras D., and Rood J.I. Conjugative transfer of RP4-oriT shuttle vectors from Escherichia coli to Clostridium perfringens. Plasmid 39 (1998) 160-164
15. Czeczulin J.R., Collie R.E., and McClane B.A. Regulated expression of Clostridium perfringens enterotoxin in naturally cpe-negative type A, B, and C isolates of C. perfringens. Infect. Immun. 64 (1996) 3301-3309
16. Lyristis M., Bryant A., Sloan J., Awad M.M., Nisbet I.T., Stevens D.L., and Rood J.I. Identification and molecular analysis of a locus that regulates extracellular toxin production in Clostridium perfringens. Mol. Microbiol. 12 (1994) 761-777
17. Jacobs T., Darji A., Frahm N., Rohde M., Wehland J., Chakraborty T., Weiss S., and Listeriolysin O. cholesterol inhibits cytolysis but not binding to cellular membranes. Mol. Microbiol. 28 (1998) 1081-1089
18. Abella B.S., Kuchinic P., Hirahoka T., and Howes D.S. Atraumatic clostridial myonecrosis: case report and literature review. J. Emerg. Med. 24 (2003) 401-405
19. Hickey M.J., Kwan R.Y., Awad M.A., Kennedy C.L., Young L.F., Hall P., Cordner L.M., Lyras D., Emmins J.J., and Rood J.I. Molecular and cellular basis of microvascular perfusion deficits induced by Clostridium perfringens and Clostridium septicum. PLoS Pathog. 4 (2008) e1000045
20. Stevens D.L., Musher D.M., Watson D.A., Eddy H., Hamill R.J., Gyorkey F., Rosen H., and Mader J. Spontaneous, nontraumatic gangrene due to Clostridium septicum. Rev. Infect. Dis. 12 (1990) 286-296