Usefulness of a novel Caco-2 cell perfusion system II. Characterization of monolayer properties and peptidase activity

Pharmazie

Volumn 64, Issue 1, 2009, Pages 36-42

  Masungi, C ^a   Mensch, J ^b   Willems, B ^b   Van Dijck, A ^b   Borremans, C ^b   Noppe, M ^b   Brewster, Marcus E ^b   Augustijns, P ^c  

^a TIBOTEC BVBA   (Belgium)

^b JANSSEN RESEARCH AND DEVELOPMENT   (Belgium)

^c DEPARTMENT OF PHARMACEUTICAL AND PHARMACOLOGICAL SCIENCES   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

AMINOPEPTIDASE; DIPEPTIDASE; PEPTIDASE; POLYCARBONATE; POLYESTER; DIPEPTIDYL PEPTIDASE; PEPTIDE HYDROLASE;

ARTICLE; CELL CULTURE; CELL DIFFERENTIATION; CELL MEMBRANE PERMEABILITY; CELL STRAIN CACO 2; CONFOCAL MICROSCOPY; CONTROLLED STUDY; ENZYME ACTIVITY; HUMAN; HUMAN CELL; UPREGULATION; ALGORITHM; CELL LINEAGE; CULTURE MEDIUM; CULTURE TECHNIQUE; CYTOLOGY; ENZYMOLOGY; IMPEDANCE; METABOLISM; PERFUSION;

ALGORITHMS; AMINOPEPTIDASES; CACO-2 CELLS; CELL CULTURE TECHNIQUES; CELL DIFFERENTIATION; CELL LINEAGE; CULTURE MEDIA; DIPEPTIDYL PEPTIDASES; ELECTRIC IMPEDANCE; HUMANS; MICROSCOPY, CONFOCAL; PEPTIDE HYDROLASES; PERFUSION;

EID: 62649089266     PISSN: 00317144     EISSN: None     Source Type: Journal    
DOI: 10.1691/ph.2008.8592     Document Type: Article

References (26)

1. Briske-Anderson MJ, Finley JW, Newman SM (1997) The influence of culture time and passage number on the morphological and physiological development of Caco-2 cells. Proc Soc Exper Biol Med 214: 248-257.
2. Chong S, Dando SA, Morrison RA (1997) Evaluation of Biocoat intestinal epithelium differentiation environment (3-day cultured Caco-2 cells) as an absorption screening model with improved productivity. Pharm Res 14: 1835-1837.
3. Fogh J, Fogh JM, Orfeo T (1977) One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J Nat Cancer Inst 59: 221-226.
4. Hauri HP, Sterchi EE, Bienz D, Fransen JA, Marxer A (1985) Expression and intracellular transport of microvillus membrane hydrolases in human intestinal epithelial cells. J Cell Biol 101: 838-851.
5. Hidalgo IJ, Li J (1996) Carrier-mediated transport and efflux mechanisms in Caco-2 cells. Adv Drug Deliv Rev 22: 53-66.
6. Howell S, Brewis IA, Hooper NM, Kenny AJ, Turner AJ (1993a) Mosaic expression of membrane peptidases by confluent cultures of Caco-2 cells. FEBS Lett 317: 109-112.
7. Howell S, Caswell AM, Kenny AJ, Turner AJ (1993b) Membrane peptidases on human osteoblast-like cells in culture: hydrolysis of calcitonin and hormonal regulation of endopeptidase-24.11. Biochem J 290: 159-164.
8. Ingels F, Deferme S, Delbar N, Oth M, Augustijns P (2002) Implementation of the Caco-2 cell culture model as a predictive tool for the oral absorption of drugs. In-house evaluation procedures. J Pharm Belg 57: 153-158.
9. Jumarie C, Malo C (1991) Caco-2 cells cultured in serum-free medium as a model for the study of enterocytic differentiation in vitro. J Cell Physiol 149: 24-33.
10. Kloth S, Ebenbeck C, Kubitza M, Schmidbauer A, Rockl W, Minuth WW (1995) Stimulation of renal microvascular development under organotypic culture conditions. FASEB J 9: 963-967.
11. Kloth S, Kobuch K, Domokos J, Wanke C, Monzer J (2000) Polar application of test substances in an organotypic environment and under continuous medium flow: a new tissue-based test concept for a broad range of applications in pharmacotoxicology. Toxicol In Vitro 14: 265-274.
12. Liang E, Chessic K, Yazdanian M (2000) Evaluation of an accelerated Caco-2 cell permeability model. J Pharm Sci 89: 336-345.
13. Masungi C, Borremans C, Willems B, Mensch J, Van Dijck A, Augustijns P, Brewster M, Noppe M (2004) Usefulness of a novel CACO-2 cell perfusion system I. In vitro prediction of the absorption potential of passively diffused compounds. J Pharm Sci 93: 2507-2521.
14. Minuth WW, Kloth S, Majer V, Dermietzel R (1994) Growth of MDCK cells on non-transparent supports. In Vitro Cell Develop Biol, Animal 30A: 12-14.
15. Minuth WW, Aigner J, Kubat B, Kloth S (1997) Improved differentiation of renal tubular epithelium in vitro: potential for tissue engineering. Exper Nephrol 5: 10-17.
16. Minuth WW, Steiner P, Strehl R, Schumacher K, de Vries U, Kloth S (1999) Modulation of cell differentiation in perfusion culture. Exper Nephrol 7: 394-406.
17. Minuth WW, Schumacher K, Strehl R, Kloth S (2000) Physiological and cell biological aspects of perfusion culture technique employed to generate differentiated tissues for long term biomaterial testing and tissue engineering. J Biomater Sci, Poly Ed 11: 495-522.
18. Risbud V, Sittinger M (2002) Tissue engineering: advances in in vitro cartilage generation. Trends Biotech 20: 351-356.
19. Rousset M (1986) The human colon carcinoma cell lines HT-29 and Caco-2: two in vitro models for the study of intestinal differentiation. Biochim 68: 1035-1040.
20. Rothen-Rutishauser B, Braun A, Gunthert M, Wunderli-Allenspach H (2000) Formation of multilayers in the caco-2 cell culture model: a confocal laser scanning microscopy study. Pharm Res 17: 460-465.
21. Schumacher K, Castrop H, Strehl R, de Vries U, Minuth WW (2002a) Cyclooxygenases in the collecting duct of neonatal rabbit kidney. Cell Physiol Biochem 12: 63-74.
22. Schumacher K, Strehl R, de Vries U, Minuth WW (2002b) Advanced technique for long term culture of epithelia in a continuous luminal-basal medium gradient. Biomaterials 23: 805-815.
23. Sittinger M, Schultz O, Keyszer G, Minuth WW, Burmester GR (1997) Artificial tissues in perfusion culture. Int J Artific Organs 20: 57-62.
24. van de Waterbeemd H, Lennernas H, Artursson P (2003) Drug Bioavailability: Estimation of Solubility, Permeability, Absorption and Bioavailability, Weinheim, Germany: Wiley-VCH, p. 576.
25. von Woedtke T, Julich WD, Alhitari N, Hanschke R, Abel PU (2002) Biosensor-controlled perfusion culture to estimate the viability of cells. Med Biol Engineer Comput 40: 704-711.
26. Yu H, Cook TJ, Sinko PJ (1997) Evidence for diminished functional expression of intestinal transporters in Caco-2 cell monolayers at high passages. Pharm Res 14: 757-762.