The cardiac glycoside binding site on the Na,K-ATPase α2 isoform plays a role in the dynamic regulation of active transport in skeletal muscle

Proceedings of the National Academy of Sciences of the United States of America

Volumn 106, Issue 8, 2009, Pages 2565-2570

  Radzyukevich, T L ^a   Lingrel, J B ^a   Heiny, J A ^a  

^a UNIVERSITY OF CINCINNATI COLLEGE OF MEDICINE   (United States)

Author keywords

Cardiotonic steroids; Endogenous cardiac glycosides; Ouabain

Indexed keywords

ADENOSINE TRIPHOSPHATASE (POTASSIUM SODIUM); ADENOSINE TRIPHOSPHATASE (POTASSIUM); CARDIAC GLYCOSIDE; DIGOXIN ANTIBODY F(AB) FRAGMENT; LIGAND;

ACTIVE TRANSPORT; ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; DRUG BINDING SITE; EXERCISE; EXTENSOR DIGITORUM LONGUS MUSCLE; GENE TARGETING; MOUSE; MUSCLE CONTRACTION; MUSCLE FIBER MEMBRANE STEADY POTENTIAL; NONHUMAN; PHYSICAL ACTIVITY; POTASSIUM TRANSPORT; PRIORITY JOURNAL; PROTEIN EXPRESSION; RUNNING; SKELETAL MUSCLE; SODIUM TRANSPORT; TASK PERFORMANCE; TISSUE SECTION; TREADMILL; WILD TYPE;

ANIMALS; BINDING SITES; BIOLOGICAL TRANSPORT, ACTIVE; CARDIAC GLYCOSIDES; ISOENZYMES; MICE; MUSCLE, SKELETAL; PHYSICAL CONDITIONING, ANIMAL; SODIUM-POTASSIUM-EXCHANGING ATPASE;

MUS; STROPHANTHUS GRATUS;

EID: 62449186907     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.0804150106     Document Type: Article

References (38)

1. Manunta P, Ferrandi M, Messaggio E, Ferrari P (2006) A new antihypertensive agent that antagonizes the prohypertensive effect of endogenous ouabain and adducin. Cardiovasc Hematol Agents Med Chem 4:61-66.
2. +-ATPase alterations in ouabain and adducin-dependent hypertension. Am J Physiol 290:R529-R535.
3. Schoner W, Scheiner-Bobis G (2007) Endogenous and exogenous cardiac glycosides and their mechanisms of action. Am J Cardiovasc Drugs 7:173-189.
4. +) ATPase associated with essential hypertension. Nature 300:650-652.
5. Moreth K, Kuske R, Renner D, Schoner W (1986) Blood pressure in essential hypertension correlates with the concentration of a circulating inhibitor of the sodium pump. Klin Wochenschr 64:239-244.
6. Bauer N, et al. (2005) Ouabain-like compound changes rapidly on physical exercise in humans and dogs: Effects of beta-blockade and angiotensin-converting enzyme inhibition. Hypertension 45:1024-1028.
7. Goto A, Yamada K (1998) Ouabain-like factor. Curr Opin Nephrol Hypertens 7:189-196.
8. Valdes R, Jr, Graves SW, Knight AB, Craig HR (1985) Endogenous digoxin imrnunoactivity is elevated in hypertensive pregnancy. Prog Clin Biol Res 192:229-232.
9. Kaplan JH (2002) Biochemistry of Na,K-ATPase. Annu Rev Biochem 71:511-535.
10. +-ATPase and biological significance of their cardiac glycoside binding site. Am J Physiol 290:R524-R528.
11. Dostanic I, et al. (2003) The alpha2 isoform of Na,K-ATPase mediates ouabain-induced cardiac inotropy in mice. J Biol Chem 278:53026-53034.
12. Price EM, Lingrel JB (1988) Structure-function relationships in the Na,K-ATPase alpha subunit: Site-directed mutagenesis of glutamine-111 to arginine and asparagine-122 to aspartic acid generates a ouabain-resistant enzyme. Biochemistry 27:8400-8408.
13. Qiu LY, et al. (2005) Reconstruction of the complete ouabain-binding pocket of Na,K-ATPase in gastric H,K-ATPase by substitution of only seven amino acids. J Biol Chem 280:32349-32355.
14. Dostanic-Larson I, Van Huysse JW, Lorenz JN, Lingrel JB (2005) The highly conserved cardiac glycoside binding site of Na,K-ATPase plays a role in blood pressure regulation. Proc Natl Acad Sci USA 102:15845-15850.
15. He S, et al. (2001) The alpha(1)- and alpha(2)-isoforms of Na-K-ATPase play different roles in skeletal muscle contractility. Am J Physiol 281:R917-R925.
16. + pump in skeletal muscle: Quantification, regulation and functional significance. Acta Physiol Scand 156:227-235.
17. Clausen T, Everts ME, Kjeldsen K (1987) Quantification of the maximum capacity for active sodium-potassium transport in rat skeletal muscle. J Physiol 388:163-181.
18. + pump activity in contracting rat muscle. J Physiol 503(Pt 3):571-581.
19. + pump regulation and skeletal muscle contractility. Physiol Rev 83:1269-1324.
20. Radzyukevich TL, et al. (2004) The Na(+)-K(+)-ATPase alpha2-subunit isoform modulates contractility in the perinatal mouse diaphragm. Am J Physiol 287:C1300-0310.
21. Lau YH, Caswell AH, Garcia M, Letellier L (1979) Ouabain binding and coupled sodium, potassium, and chloride transport in isolated transverse tubules of skeletal muscle. J Gen Physiol 74:335-349.
22. Clausen T (2003) The sodium pump keeps us going. Ann NY Acad Sci 986:595-602.
23. Morris RG, Hoorn EJ, Knepper MA (2006) Hypokalemia in a mouse model of Gitelman's syndrome. Am J Physiol 290:F1416-F1420.
24. Arrighi I, et al. (2001) Altered potassium balance and aldosterone secretion in a mouse model of human congenital long QT syndrome. Proc Natl Acad Sci USA 98:8792-8797.
25. Hnik P, et al (1976) Work-induced potassium changes in skeletal muscle and effluent venous blood assessed by liquid ion-exchanger microelectrodes. Pflugers Arch 362:85-94.
26. + balance of the gastrocnemius muscle of the dog during exercise. Pflugers Arch 387:231-237.
27. Juel C (1986) Potassium and sodium shifts during in vitro isometric muscle contraction, and the time course of the ion-gradient recovery. Pflugers Arch 406:458-463.
28. Williams MW, et al, (2001) Na,K-ATPase in skeletal muscle: Two populations of beta-spectrin control localization in the sarcolemma but not partitioning between the sarcolemma and the transverse tubules. J Cell Sci 114:751-762.
29. 86Rb is not a reliable tracer for potassium in skeletal muscle. Biochem J 302 (Pt 3):745-751.
30. + pump in rat skeletal muscle. Am J Physiol 266:C925-C934.
31. Pullen MA, Harpel MR, Danoff TM, Brooks DP (2008) Comparison of non-digitalis binding properties of digoxin-specific Fabs using direct binding methods. J Immunol Methods 336(2):235-241.
32. 3H] ouabain binding in rat skeletal muscle in vivo. J Physiol 333:367-381.
33. Hicks A, McComas AJ (1989) Increased sodium pump activity following repetitive stimulation of rat soleus muscles. J Physiol 414:337-349.
34. + homeostasis during exercise in humans. J Physiol 586:1447-1459.
35. Schoner W, Scheiner-Bobis G (2007) Endogenous and exogenous cardiac glycosides: Their roles in hypertension, salt metabolism, and cell growth. Am J Physiol 293:509-536.
36. Billat VL, Mouisel E, Roblot N, Melki J (2005) Inter-and intrastrain variation in mouse critical running speed. J Appl Physiol 98:1258-1263.
37. Cougnon MH, Moseley AE, Radzyukevich TL, Lingrel JB, Heiny JA (2002) Na, K-ATPase alpha-and beta-isoform expression in developing skeletal muscles: Alpha(2) correlates with t-tubule formation. Pflugers Arch 445:123-131.
38. Burkholder TJ, Fingado B, Baron S, Lieber RL (1994) Relationship between musclefiber types and sizes and muscle architectural properties in the mouse hindlimb. J Morphol 221:177-190.