Bacterial artificial chromosomes improve recombinant protein production in mammalian cells

BMC Biotechnology

Volumn 9, Issue , 2009, Pages

  Blaas, Leander ^a   Musteanu, Monica ^a   Eferl, Robert ^a   Bauer, Anton ^b   Casanova, Emilio ^a  

^a MEDICAL UNIVERSITY OF VIENNA   (Austria)

^b Babraham Research Campus   (Austria)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL CULTURE; BACTERIOLOGY; CELL CULTURE; CHROMOSOMES; FARM BUILDINGS; MAMMALS; PROTEINS;

BACTERIAL ARTIFICIAL CHROMOSOMES; CELL CLONES; CELL LINES; CRITICAL STEPS; EXPRESSION VECTORS; LARGE SCALE PROTEINS; MAMMALIAN CELLS; PROTEIN PRODUCTIONS; PROTEIN YIELDS; RECOMBINANT PROTEIN PRODUCTIONS; STABLE CELL LINES;

VECTORS;

IMMUNOGLOBULIN FC FRAGMENT; IMMUNOGLOBULIN G1; RECOMBINANT PROTEIN; IMMUNOGLOBULIN G;

ANTIBODY PRODUCTION; ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; CELL CLONE; CELL LINE; CELL STRAIN HEK293; CONTROLLED STUDY; EXPRESSION VECTOR; GENE DUPLICATION; GENE LOCUS; GENETIC RECOMBINATION; HUMAN; HUMAN CELL; MAMMAL CELL; PROTEIN SYNTHESIS; TRANSGENE; BIOSYNTHESIS; GENE VECTOR; GENETIC ENGINEERING; METHODOLOGY;

BACTERIA (MICROORGANISMS); MAMMALIA;

CELL LINE; CHROMOSOMES, ARTIFICIAL, BACTERIAL; GENETIC ENGINEERING; GENETIC VECTORS; HUMANS; IMMUNOGLOBULIN G; RECOMBINANT PROTEINS;

EID: 62349130284     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-9-3     Document Type: Article

References (12)

1. Wurm FM Production of recombinant protein therapeutics in cultivated mammalian cells Nature biotechnology 2004, 22(11):1393-1398. 10.1038/ nbt1026 15529164
2. Kwaks TH Barnett P Hemrika W Siersma T Sewalt RG Satijn DP Brons JF van Blokland R Kwakman P Kruckeberg AL Identification of anti-repressor elements that confer high and stable protein production in mammalian cells Nature biotechnology 2003, 21(5):553-558. 10.1038/nbt814 12679786
3. Huang Y Li Y Wang YG Gu X Wang Y Shen BF An efficient and targeted gene integration system for high-level antibody expression Journal of immunological methods 2007, 322(1-2):28-39. 10.1016/j.jim.2007.01.022 17350648
4. Giraldo P Montoliu L Size matters: Use of YACs, BACs and PACs in transgenic animals Transgenic research 2001, 10(2):83-103. 10.1023/ A:1008918913249 11305364
5. Copeland NG Jenkins NA Court DL Recombineering: A powerful new tool for mouse functional genomics Nature reviews 2001, 2(10):769-779. 11584293
6. Yang XW Model P Heintz N Homologous recombination based modification in Escherichiacoli and germline transmission in transgenic mice of a bacterial artificial chromosome Nature biotechnology 1997, 15(9):859-865. 10.1038/nbt0997-859 9306400
7. Zhang Y Buchholz F Muyrers JP Stewart AF A new logic for DNA engineering using recombination in Escherichia coli Nature genetics 1998, 20(2):123-128. 10.1038/2417 9771703
8. Blaas L Musteanu M Zenz R Eferl R Casanova E PhiC31-mediated cassette exchange into a bacterial artificial chromosome BioTechniques 2007, 43(5):659-660. 10.2144/000112592 18072595
9. Niwa H Yamamura K Miyazaki J Efficient selection for high-expression transfectants with a novel eukaryotic vector Gene 1991, 108(2):193-199. 10.1016/0378-1119(91)90434-D 1660837
10. Felgenhauer M Kohl J Ruker F Nucleotide sequences of the cDNAs encoding the V-regions of H- and L-chains of a human monoclonal antibody specific to HIV-1-gp41 Nucleic acids research 1990, 18(16):4927. 331992 1697678 10.1093/nar/18.16.4927
11. Soriano P Generalized lacZ expression with the ROSA26 Cre reporter strain Nature genetics 1999, 21(1):70-71. 10.1038/5007 9916792
12. Zagranichnaya TK Wu X Danos AM Villereal ML Gene expression profiles in HEK-293 cells with low or high store-operated calcium entry: Can regulatory as well as regulated genes be identified? Physiological genomics 2005, 21(1):14 -33. 10.1152/physiolgenomics.00099.2004 15623568