A non-invasive technique for quantifying and isolating fused cells

Cytotechnology

Volumn 58, Issue 3, 2008, Pages 113-118

  Hu, Lulin ^a   Plafker, Kendra ^a   Henthorn, James ^b   Ceresa, Brian P ^a,b  

^a UNIVERSITY OF OKLAHOMA COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF OKLAHOMA HEALTH SCIENCES CENTER   (United States)

Author keywords

Cell cell fusion; Fluorescence associated cell sorting; Heterokaryons

Indexed keywords

RNA POLYMERASE; YELLOW FLUORESCENT PROTEIN;

ARTICLE; BACTERIOPHAGE T7; CELL ASSAY; CELL FATE; CELL FUSION; CELL GROWTH; CELL ISOLATION; CELL SELECTION; CELL TRANSFORMATION; CHROMOSOME NUMBER; CONTROLLED STUDY; FLOW CYTOMETRY; FLUORESCENCE MICROSCOPY; GENETIC TRANSFECTION; GROWTH RATE; HUMAN; HUMAN CELL; NONHUMAN; PROTEIN EXPRESSION;

EID: 62149084845     PISSN: 09209069     EISSN: 15730778     Source Type: Journal    
DOI: 10.1007/s10616-009-9186-z     Document Type: Article

References (15)

1. EH Chen EN Olson 2005 Unveiling the mechanisms of cell-cell fusion Science 308 369 373 10.1126/science.1104799
2. H Damke T Baba DE Warnock SL Schmid 1994 Induction of mutant dynamin specifically blocks endocytic coated vesicle formation J Cell Biol 127 915 934 10.1083/jcb.127.4.915
3. JL Dinneen BP Ceresa 2004 Constitutive activation of rab5 results in a ligand independent redistribution of the EGFR and attenuates its ability to signal Traffic 5 606 615 10.1111/j.1398-9219.2004.00204.x
4. D Duelli Y Lazebnik 2007 Cell-to-cell fusion as a link between viruses and cancer Nat Rev Cancer 7 968 976 10.1038/nrc2272
5. DM Duelli HM Padilla-Nash D Berman KM Murphy T Ried Y Lazebnik 2007 A virus causes cancer by inducing massive chromosomal instability through cell fusion Curr Biol 17 1 7 10.1016/j.cub.2007.01.049
6. M Gossen H Bujard 1992 Tight control of gene expression in mammalian cells by tetracycline-responsive promoters Proc Natl Acad Sci USA 89 5547 5551 10.1073/pnas.89.12.5547
7. L Hu K Plafker V Vorozhoko RE Zuna MH Hanigan GJ Gorbsky SM Plafker PC Angeletti BP Ceresa 2009 Human Papillomavirus 16 E5 induces polyploidy by cell fusion Virology 384 125 134 10.1016/j.virol.2008.10.011
8. J Kimpton M Emerman 1992 Detection of replication-competent and peudotyped human immunodeficiency virus with a sensitive cell line on the basis of activation of an integrated β-galactosidase gene J Virol 66 2232 2239
9. AP Madan DB De Franco 1993 Bidirectional transport of glucocorticoid receptors across the nuclear envelope Proc Natl Acad Sci USA 90 3588 3592 10.1073/pnas.90.8.3588
10. O Nussbaum CC Broder EA Berger 1994 Fusogenic mechanisms of enveloped-virus glycoproteins analyzed by a novel recombinant vaccinia virus-based assay quantitating cell fusion-dependent reporter gene activation J Virol 68 5411 5422
11. GE Parris 2008 Cell-cell fusion is the rate-limiting step in causation and progression of clinically significant cancers Cancer Genet Cytogenet 185 113 10.1016/j.cancergencyto.2008.05.008
12. GE Parris 2008 Chromosomal instability (CIN) leads to clone extinction, not cancer Med Hypotheses 71 983 10.1016/j.mehy.2008.07.046
13. JM Pawelek AK Chakraborty 2008 The cancer cell-leukocyte fusion theory of metastasis Adv Cancer Res 101 397 444 10.1016/S0065-230X(08)00410-7
14. JM Pawelek AK Chakraborty 2008 Fusion of tumour cells with bone marrow-derived cells: a unifying explanation for metastasis Nat Rev Cancer 8 377 386 10.1038/nrc2371
15. T Sakamoto H Ushijima S Okitsu E Suzuki K Sakai S Morikawa WE Muller 2003 Establishment of an HIV cell-cell fusion assay by using two genetically modified HeLa cell lines and reporter gene J Virol Methods 114 159 166 10.1016/j.jviromet.2003.08.016