Establishment of an HIV cell-cell fusion assay by using two genetically modified HeLa cell lines and reporter gene

Journal of Virological Methods

Volumn 114, Issue 2, 2003, Pages 159-166

  Sakamoto, Tatsunori ^a   Ushijima, Hiroshi ^a   Okitsu, Shoko ^a   Suzuki, Eiko ^a   Sakai, Koji ^b   Morikawa, Shigeru ^b   Müller, Werner E G ^c  

^a UNIVERSITY OF TOKYO   (Japan)

^b NATIONAL INSTITUTE OF INFECTIOUS DISEASES   (Japan)

^c JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

Cell fusion; HeLa cell; Human immunodeficiency virus; Reporter gene

Indexed keywords

CD4 ANTIGEN; CHEMOKINE RECEPTOR CCR4; ENVELOPE PROTEIN;

ACCURACY; ARTICLE; CELL INTERACTION; CONTROLLED STUDY; ENVELOPE GENE; ENZYME ACTIVITY; FUSION GENE; GENE FUSION; HELA CELL; HUMAN; HUMAN CELL; HUMAN IMMUNODEFICIENCY VIRUS 1; MOLECULAR MODEL; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; REPORTER GENE; VACCINIA;

HUMAN IMMUNODEFICIENCY VIRUS; VACCINIA VIRUS;

EID: 0242475091     PISSN: 01660934     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jviromet.2003.08.016     Document Type: Article

References (41)

1. Alkhatib G., Combadiere C., Broder C.C., Feng Y., Kennedy P.E., Murphy P.M., Berger E.A. CC CKR5: a RANTES, MIP-1alpha, MIP-1beta receptor as a fusion cofactor for macrophage-tropic HIV-1. Science. 272:1996;1955-1958.
2. Aoki Y., Aizaki H., Shimoike T., Tani H., Ishii K., Saito I., Matsuura Y., Miyamura T. A human liver cell line exhibits efficient translation of HCV RNAs produced by a recombinant adenovirus expressing T7 RNA polymerase. Virology. 250:1998;140-150.
3. Ashorn P.A., Berger E.A., Moss B. Human immunodeficiency virus envelope glycoprotein/CD4-mediated fusion of nonprimate cells with human cells. J. Virol. 64:1990;2149-2156.
4. Ayehunie S., Groves R.W., Bruzzese A.M., Ruprecht R.M., Kupper T.S., Langhoff E. Acutely infected Langerhans cells are more efficient than T cells in disseminating HIV type 1 to activated T cells following a short cell-cell contact. AIDS Res. Hum. Retrovir. 11:1995;877-884.
5. Barre-Sinoussi F., Chermann J.C., Rey F., Nugeyre M.T., Chamaret S., Gruest J., Dauguet C., Axler-Blin C., Vezinet-Brun F., Rouzioux C., Rozenbaum W., Montagnier L. Isolation of a T-lymphotropic retrovirus from a patient at risk for acquired immune deficiency syndrome (AIDS). Science. 220:1983;868-871.
6. Broder C.C., Collman R.G. Chemokine receptors and HIV. J. Leukoc. Biol. 62:1997;20-29.
7. Carrillo A., Ratner L. Cooperative effects of the human immunodeficiency virus type 1 envelope variable loops V1 and V3 in mediating infectivity for T cells. J. Virol. 70:1996;1310-1316.
8. Chan D.C., Kim P.S. HIV entry and its inhibition. Cell. 93:1998;681-684.
9. Chiba H., Asanuma S., Okamoto M., Inokoshi J., Tanaka H. A simple screening systems for anti-HIV drugs: syncytium formation assay using T-cell line tropic and macrophage-tropic HIV env expressing cell lines-establishment and validation. J. Antibiot. 54:2001;818-826.
10. Ciminale V., Felber B.K., Campbell M., Pavlakis G.N. A bioassay for HIV-1 based on Env-CD4 interaction. AIDS Res. Hum. Retrovir. 6:1990;1281-1287.
11. Deng H., Liu R., Ellmeier W., Choe S., Unutmaz D., Burkhart M., Di Marzio P., Marmon S., Sutton R.E., Hill C.M., Davis C.B., Peiper S.C., Schall T.J., Littman D.R., Landau N.R. Identification of a major co-receptor for primary isolates of HIV-1. Nature. 381:1996;661-666.
12. Dragic T., Charneau P., Clavel F., Alizon M. Complementation of murine cells for human immunodeficiency virus envelope/CD4-mediated fusion in human/murine heterokaryons. J. Virol. 66:1992;4794-4802.
13. Dragic T., Litwin V., Allaway G.P., Martin S.R., Huang Y., Nagashima K.A., Cayanan C., Maddon P.J., Koup R.A., Moore J.P., Paxton W.A. HIV-1 entry into CD4+ cells is mediated by the chemokine receptor CC-CKR-5. Nature. 381:1996;667-673.
14. Endres M.J., Clapham P.R., Marsh M., Ahuja M., Turner J.D., McKnight A., Thomas J.F., Stoebenau-Haggarty B., Choe S., Vance P.J., Wells T.N., Power C.A., Sutterwala S.S., Doms R.W., Landau N.R., Hoxie J.A. CD4-independent infection by HIV-2 is mediated by fusin/CXCR4. Cell. 87:1996;745-756.
15. Feng Y., Broder C.C., Kennedy P.E., Berger E.A. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science. 272:1996;872-877.
16. Gartner S., Markovits P., Markovitz D.M., Kaplan M.H., Gallo R.C., Popovic M. The role of mononuclear phagocytes in HTLV-III/LAV infection. Science. 233:1986;215-219.
17. Gervaix A., West D., Leoni L.M., Richman D.D., Wong-Staal F., Corbeil J. A new reporter cell line to monitor HIV infection and drug susceptibility in vitro. Proc. Natl. Acad. Sci. U.S.A. 94:1997;4653-4658.
18. Ghorpade A., Xia M.Q., Hyman B.T., Persidsky Y., Nukuna A., Bock P., Che M., Limoges J., Gendelman H.E., Mackay C.R. Role of the beta-chemokine receptors CCR3 and CCR5 in human immunodeficiency virus type 1 infection of monocytes and microglia. J. Virol. 72:1998;3351-3361.
19. Harouse J.M., Gonzalez-Scarano F. Infection of SK-N-MC cells, a CD4-negative neuroblastoma cell line, with primary human immunodeficiency virus type 1 isolates. J. Virol. 70:1996;7290-7294.
20. Helseth E., Kowalski M., Gabuzda D., Olshevsky U., Haseltine W., Sodroski J. Rapid complementation assays measuring replicative potential of human immunodeficiency virus type 1 envelope glycoprotein mutants. J. Virol. 64:1990;2416-2420.
21. Hoffman T.L., Doms R.W. Chemokines and coreceptors in HIV/SIV-host interactions. AIDS. 12(Suppl. A):1998;S17-S26.
22. Hong Y.L., Wu L.H., Cui M., McMaster G., Hunt S.W. 3rd, Chung F.Z. New reporter cell lines to study macrophage-tropic HIV envelope protein-mediated cell-cell fusion. AIDS Res. Hum. Retrovir. 15:1999;1667-1672.
23. Horuk R., Hesselgesser J., Zhou Y., Faulds D., Halks-Miller M., Harvey S., Taub D., Samson M., Parmentier M., Rucker J., Doranz B.J., Doms R.W. The CC chemokine I-309 inhibits CCR8-dependent infection by diverse HIV-1 strains. J. Biol. Chem. 273:1998;386-391.
24. Hung C.S., Vander Heyden N., Ratner L. Analysis of the critical domain in the V3 loop of human immunodeficiency virus type 1 gp120 involved in CCR5 utilization. J. Virol. 73:1999;8216-8226.
25. Isaacs S.N., Yi Y., Singh A., Collman R.G. A macrophage fusion assay for rapid screening of cloned HIV-1 Env using dual recombinant vaccinia viruses expressing distinct RNA polymerases. J. Virol. Methods. 81:1999;55-61.
26. Kimpton J., Emerman M. Detection of replication-competent and pseudotyped human immunodeficiency virus with a sensitive cell line on the basis of activation of an integrated beta-galactosidase gene. J. Virol. 66:1992;2232-2239.
27. Luciw, P.A., 1996. Human immunodeficiency viruses and their replication. In: Fields, B.N., Knipe, D.M., Moley, P.M. (Eds.), Fields Virology, third ed. Lippincott-Raven, New York, pp. 1881-1952.
28. Moses A.V., Stenglein S.G., Strussenberg J.G., Wehrly K., Chesebro B., Nelson J.A. Sequences regulating tropism of human immunodeficiency virus type I for brain capillary endothelial cells map to a unique region on the viral genome. J. Virol. 70:1996;3401-3406.
29. Nussbaum O., Broder C.C., Berger E.A. Fusogenic mechanisms of enveloped-virus glycoproteins analyzed by a novel recombinant vaccinia virus-based assay quantitating cell fusion-dependent reporter gene activation. J. Virol. 68:1994;5411-5422.
30. O'Brien W.A., Koyanagi Y., Namazie A., Zhao J.Q., Diagne A., Idler K., Zack J.A., Chen I.S. HIV-1 tropism for mononuclear phagocytes can be determined by regions of gp120 outside the CD4-binding domain. Nature. 348:1990;69-73.
31. Page K.A., Landau N.R., Littman D.R. Construction and use of a human immunodeficiency virus vector for analysis of virus infectivity. J. Virol. 64:1990;5270-5276.
32. Pope M., Frankel S.S., Mascola J.R., Trkola A., Isdell F., Birx D.L., Burke D.S., Ho D.D., Moore J.P. Human immunodeficiency virus type 1 strains of subtypes B and E replicate in cutaneous dendritic cell-T-cell mixtures without displaying subtype-specific tropism. J. Virol. 71:1997;8001-8007.
33. Rocancourt D., Bonnerot C., Jouin H., Emerman M., Nicolas J.F. Activation of a beta-galactosidase recombinant provirus: application to titration of human immunodeficiency virus (HIV) and HIV-infected cells. J. Virol. 64:1990;2660-2668.
34. Rucker J., Edinger A.L., Sharron M., Samson M., Lee B., Berson J.F., Yi Y., Margulies B., Collman R.G., Doranz B.J., Parmentier M., Doms R.W. Utilization of chemokine receptors, orphan receptors, and herpesvirus-encoded receptors by diverse human and simian immunodeficiency viruses. J. Virol. 71:1997a;8999-9007.
35. Rucker J., Doranz B.J., Edinger A.L., Long D., Berson J.F., Doms R.W. Cell-cell fusion assay to study role of chemokine receptors in human immunodeficiency virus type 1. Methods Enzymol. 288:1997b;118-133.
36. Schuitemaker H., Kootstra N.A., de Goede R.E., de Wolf F., Miedema F., Tersmette M. Monocytotropic human immunodeficiency virus type 1 (HIV-1) variants detectable in all stages of HIV-1 infection lack T-cell line tropism and syncytium-inducing ability in primary T-cell culture. J. Virol. 65:1991;356-363.
37. Sester M., Burgert H.G. Conserved cysteine residues within the E3/19K protein of adenovirus type 2 are essential for binding to major histocompatibility complex antigens. J. Virol. 68:1994;5423-5432.
38. Shoji I., Aizaki H., Tani H., Ishii K., Chiba T., Saito I., Miyamura T., Matsuura Y. Efficient gene transfer into various mammalian cells, including non-hepatic cells, by baculovirus vectors. J. Gen. Virol. 78:1997;2657-2664.
39. Takikawa S., Ishii K., Aizaki H., Suzuki T., Asakura H., Matsuura Y., Miyamura T. Cell fusion activity of hepatitis C virus envelope proteins. J. Virol. 74:2000;5066-5074.
40. Verrier F.C., Charneau P., Altmeyer R., Laurent S., Borman A.M., Girard M. Antibodies to several conformation-dependent epitopes of gp120/gp41 inhibit CCR-5-dependent cell-to-cell fusion mediated by the native envelope glycoprotein of a primary macrophages-tropic HIV-1 isolate. Proc. Natl. Acad. Sci. U.S.A. 94:1997;9326-9331.
41. Weiler B., Schacke H., Bachman M., Brigido L., Gilbert M., Mills J., Matthes E., Forrest J.M.S., Muller W.E.G. Human immunodeficiency virus: novel enzyme-linked immunoassays for quantitation of envelope glycoprotein 120. J. Virol. Methods. 32:1991;287-304.