Measurement of acid polysaccharides (APS) associated with microphytobenthos in salt marsh sediments

Aquatic Microbial Ecology

Volumn 54, Issue 2, 2009, Pages 185-198

  Thornton, Daniel C O ^a   Visser, Lindsey A ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

Alcian blue; Colloidal carbohydrate; EPS; Exopolymers; Gulf of Mexico; Microphytobenthos; Subtropics

Indexed keywords

EID: 61949258066     PISSN: 09483055     EISSN: None     Source Type: Journal    
DOI: 10.3354/ame01265     Document Type: Article

References (42)

1. Abdullahi AS, Underwood GJC, Gretz MR (2006) Extracellular matrix assembly in diatoms (Bacillariophyceae). V. Environmental effects on polysaccharide synthesis in the model diatom, Phaeodactylum tricornutum. J Phycol 42:363-378
2. Bellinger BJ, Abdullahi AS, Gretz MR, Underwood GJC (2005) Biofilm polymers: relationship between carbohydrate biopolymers from estuarine mudflats and unialgal cultures of benthic diatoms. Aquat Microb Ecol 38:169-180
3. Brotas V, Caterino F (1995) Microphytobenthos primary production of Tagus estuary intertidal flats (Portugal). Neth J Aquat Ecol 29:333-339
4. Brotas V, Mendes CR, Cartaxana P (2007) Microphytobenthic biomass assessment by pigment analysis: comparison of spectrophotometry and high performance liquid chromatography methods. Hydrobiologia 587:19-24
5. Chiovitti A, Molino P, Crawford SA, Teng R, Spueck T, Wetherbee R (2004) The glucans extracted with warm water from diatoms are mainly derived from intracellular chrysolaminaran and not extracellular polysaccharides. Eur J Phycol 39:117-128
6. Claquin P, Probert I, Lefebvre S, Veron B (2008) Effects of temperature on photosynthetic parameters and TEP production in eight species of marine microalgae. Aquat Microb Ecol 51:1-11
7. Crayton MA (1982) A comparative cytochemical study of volvocacean matrix polysaccharides. J Phycol 18:336-344
8. De Brouwer JFC, Stal LJ (2004) Does warm-water extraction of benthic diatoms yield extracellular polymeric substances or does it extract intracellular chrysolaminaran? Eur J Phycol 39:129-131
9. De Brouwer JFC, Wolfstein K, Stal LJ (2002) Physical characterization and diel dynamics of different fractions of extracellular polysaccharides in an axenic culture of a benthic diatom. Eur J Phycol 37:37-44
10. De Jonge VN, Colijn F (1994) Dynamics of microphytobenthos biomass in the Ems Estuary. Mar Ecol Prog Ser 104:185-196
11. De Jonge VN, Van Beusekom JEE (1992) Contribution of resuspended microphytobenthos to total phytoplankton in the Ems Estuary and its possible role for grazers. Neth J Sea Res 30:91-105
12. Dubois M, Gilles KA, Hamilton JK, Rebers PA, Smith F (1956) Colorimetric method for determination of sugars and related substances. Anal Chem 28:350-356
13. Falkowski P, Scholes RJ, Boyle E, Canadell J and others (2000) The global carbon cycle: a test of our knowledge of earth as a system. Science 290:291-296
14. Glud RN, Kühl M, Wenzhöfer F, Rysgaard S (2002) Benthic diatoms of a high Arctic fjord (Young Sound, NE Greenland): importance for ecosystem primary production. Mar Ecol Prog Ser 238:15-29
15. Guarini JM, Gros P, Blanchard G, Richard P, Fillon A (2004) Benthic contribution to pelagic microalgal communities in two semi-enclosed, European-type littoral ecosystems (Marennes-Oleron Bay and Aiguillon Bay, France). J Sea Res 52:241-258
16. Hanlon ARM, Bellinger B, Haynes K, Xiao G and others (2006) Dynamics of extracellular polymeric substance (EPS) production and loss in an estuarine, diatom-dominated, microalgal biofilm over a tidal emersion-immersion period. Limnol Oceanogr 51:79-93
17. Haynes K, Hofmann TA, Smith CJ, Ball AS, Underwood GJC, Osborn AM (2007) Diatom-derived carbohydrates as factors affecting bacterial community composition in estuarine sediments. Appl Environ Microbiol 73:6112-6124
18. Holland AF, Zingmark RG, Dean JM (1974) Quantitative evidence concerning stabilization of sediments by marine benthic diatoms. Mar Biol 27:191-196
19. Hung CC, Guo LD, Santschi PH, Alvarado-Quiroz N, Haye JM (2003) Distributions of carbohydrate species in the Gulf of Mexico. Mar Chem 81:119-135
20. Jahnke RA, Nelson JR, Marinelli RL, Eckman JE (2000) Benthic flux of biogenic elements on the southeastern US continental shelf: influence of pore water advective transport and benthic microalgae. Cont Shelf Res 20:109-127
21. MacIntyre HL, Geider RJ, Miller DC (1996) Microphytobenthos: the ecological role of the 'secret garden' of unvegetated, shallow-water marine habitats.1. Distribution, abundance and primary production. Estuaries 19:186-201
22. 13C-labeling study. Limnol Oceanogr 45:1224-1234
23. Passow U (2002) Transparent exopolymer particles (TEP) in aquatic environments. Prog Oceanogr 55:287-333
24. Passow U, Alldredge AL (1995) A dye-binding assay for the spectrophotometric measurement of transparent exopolymer particles (TEP). Limnol Oceanogr 40:1326-1335
25. Paterson DM (1989) Short-term changes in the erodibility of intertidal cohesive sediments related to the migratory behavior of epipelic diatoms. Limnol Oceanogr 34:223-234
26. Pinckney JL, Lee AR (2008) Spatiotemporal patterns of subtidal benthic microalgal biomass and community composition in Galveston Bay, Texas, USA. Estuaries Coasts 31:444-454
27. Pinckney JL, Carman KR, Lumsden SE, Hymel SN (2003) Microalgal-meiofaunal trophic relationships in muddy intertidal estuarine sediments. Aquat Microb Ecol 31:99-108
28. Ramus J (1977) Alcian blue - quantitative aqueous assay for algal acid and sulfated polysaccharides. J Phycol 13:345-348
29. Santos PJP, Castel J, Souza-Santos LP (1997) Spatial distribution and dynamics of microphytobenthos biomass in the Gironde estuary (France). Oceanol Acta 20:549-556
30. 234Th and POC export fluxes by marine organisms. Geophys Res Lett 30, 1044, doi:10.1029/2002GL016046
31. Smith DJ, Underwood GJC (1998) Exopolymer production by intertidal epipelic diatoms. Limnol Oceanogr 43:1578-1591
32. Stal LJ (2003) Microphytobenthos, their extracellular polymeric substances, and the morphogenesis of intertidal sediments. Geomicrobiol J 20:463-478
33. Stal LJ, Vangemerden H, Krumbein WE (1984) The simultaneous assay of chlorophyll and bacteriochlorophyll in natural microbial communities. J Microbiol Methods 2:295-306
34. Takai N, Yorozu A, Tanimoto T, Hoshika A, Yoshihara K (2004) Transport pathways of microphytobenthos-originating organic carbon in the food web of an exposed hard bottom shore in the Seto Inland Sea, Japan. Mar Ecol Prog Ser 284:97-108
35. Thornton DCO, Dong LF, Underwood GJC, Nedwell DB (2002) Factors affecting microphytobenthic biomass, species composition and production in the Colne Estuary (UK). Aquat Microb Ecol 27:285-300
36. Thornton DCO, Fejes EM, DiMarco SF, Clancy KM (2007) Measurement of acid polysaccharides in marine and freshwater samples using alcian blue. Limnol Oceanogr Methods 5:73-87
37. Tolhurst TJ, Defew EC, De Brouwer JFC, Wolfstein K, Stal LJ, Paterson DM (2006) Small-scale temporal and spatial variability in the erosion threshold and properties of cohesive intertidal sediments. Cont Shelf Res 26:351-362
38. Underwood GJC, Kromkamp J (1999) Primary production by phytoplankton and microphytobenthos in estuaries. Adv Ecol Res 29:93-153
39. Underwood GJC, Paterson DM (2003) The importance of extracellular carbohydrate production by marine epipelic diatoms. Adv Bot Res 40:183-240
40. Underwood GJC, Paterson DM, Parkes RJ (1995) The measurement of microbial carbohydrate exopolymers from intertidal sediments. Limnol Oceanogr 40:1243-1253
41. Verdugo P, Alldredge AL, Azam F, Kirchman DL, Passow U, Santschi PH (2004) The oceanic gel phase: a bridge in the DOM-POM continuum. Mar Chem 92:67-85
42. Werner U, Blazejak A, Bird P, Eickert G and others (2008) Microbial photosynthesis in coral reef sediments (Heron Reef, Australia). Estuar Coast Shelf Sci 76:876-888