Measurement of acid polysaccharides in marine and freshwater samples using alcian blue

Limnology and Oceanography: Methods

Volumn 5, Issue FEB, 2007, Pages 73-87

  Thornton, Daniel C O ^a   Fejes, Elizabeth M ^a   Dimarco, Steven F ^a   Clancy, Kristen M ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

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[No Author keywords available]

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PROTISTA;

EID: 44649111211     PISSN: 15415856     EISSN: 15415856     Source Type: Journal    
DOI: 10.4319/lom.2007.5.73     Document Type: Article

References (45)

1. Abdullahi, A. S., G. J. C. Underwood, and M. R. Gretz. 2006. Extracellular matrix assembly in diatoms (Bacillariophyceae) versus environmental effects on polysaccharide synthesis in the model diatom, Phaeodactylum tricornutum. J. Phycol. 42:363-378.
2. Alldredge, A. L., U. Passow, and B. E. Logan. 1993. The abundance and significance of a class of large, transparent organic particles in the ocean. Deep Sea Res. 140:1131-1140.
3. Apoya-Horton, M. D., L. Yin, G.J. C. Underwood, and M. R. Gretz. 2006. Movement modalities and responses to environmental changes of the mudflat diatom Cylindrotheca closterium (Bacillariophyceae). J. Phycol. 42:379-390.
4. Berger, B., B. Hoch, G. Kavka, and G. J. Herndl. 1996. Bacterial colonization of suspended solids in the River Danube. Aquat. Microb. Ecol. 10:37-44.
5. Buesseler, K., L. Ball, J. Andrews, C. Benitez-Nelson, R. Belastock, F. Chai, and Y. Chao. 1998. Upper ocean export of particulate organic carbon in the Arabian Sea derived from thorium234. Deep Sea Res. II 45:2461-2487.
6. 234Th as a POC proxy. Mar. Chem. 100:213-233.
7. Chin, W. -C., M. V. Orellana, and P. Verdugo. 1998. Spontaneous assembly of marine dissolved organic matter into polymer gels. Nature 391:568-572.
8. Corzo, A., J. A. Morillo, and S. Rodriguez. 2000. Production of transparent exopolymer particles (TEP) in cultures of Chaetoceros calcitrans under nitrogen limitation. Aquat. Microb. Ecol. 23:63-72.
9. Crayton, M. A. 1982. A comparative cytochemical study of volvocean matrix polysaccharides. J. Phycol. 18:336-344.
10. Dubois, M., K. A. Gilles, J. K. Hamilton, P. A. Rebers, and F. Smith. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28:350-356.
11. Engel, A. 2000. The role of transparent exopolymer particles (TEP) in the increase in apparent stickiness (α) during the decline of a diatom bloom. J. Plankton Res. 22:485-497.
12. Falkowski, P., R. J. Scholes, E. Boyle, J. Canadell, D. Canfield, J. Elser, N. Gruber, H. Hibbard, P. Högberg, S. Linder, F. T. Mackenzie, B. Moore III, T. Pedersen, Y. Rosenthal, S. Seitzinger, V. Smetacek, and W. Steffen. 2000. The global carbon cycle: A test of our knowledge of Earth as a system. Science. 290:291-296
13. Grossart, H. -P., M. Simon, and B. E. Logan. 1997. Formation of macroscopic organic aggregates (lake snow) in a large lake: The significance of transparent exopolymer particles, phytoplankton, and zooplankton. Limnol. Oceanogr. 42:1651-1659.
14. _, T. Berman, M. Simon, and K. Pohlmann. 1998. Occurrence and microbial dynamics of macroscopic organic aggregates (lake snow) in Lake Kinneret, Israel, in fall. Aquat. Microb. Ecol. 14:59-67.
15. 234Th scavenging and its relationship to acid polysaccharide abundance in the Gulf of Mexico. Mar. Chem. 78:103-119.
16. Harrison, P. J., R. E. Waters, and F. J. R. Taylor. 1980. A broad spectrum artificial seawater medium for coastal and open ocean phytoplankton. J. Phycol. 16:28-35.
17. Hayat, M. A. 2000. Positive staining, p. 242-260. In M. A. Hayat [ed.], Principles and techniques of electron microscopy: Biological applications. 4th ed. Cambridge Univ. Press.
18. Hong, Y., W. O. Smith, and A.-M. White. 1997. Studies on transparent exopolymeric particles (TEP) produced in the Ross Sea (Antarctica) and by Phaeocystis antartica (Prymnesiophyceae). J. Phycol. 33:368-376.
19. Hung, C. -C., L. Guo, P. H. Santschi, N. Alvarado-Quiroz, and J. M. Haye. 2003. Distributions of carbohydrate species in the Gulf of Mexico. Mar. Chem. 81:119-135.
20. Kiene, R. P., and L. J. Linn. 1999. Filter-type and sample handling affect determination of organic substrate uptake by bacterioplankton. Aquat. Microb. Ecol. 27:285-300.
21. Kiørboe, T., P. Tiselius, B. Mitchell-Innes, J. L. S. Hansen, A. W. Visser, and X. Mari. 1998. Intensive aggregate formation with low vertical flux during an upwelling-induced diatom bloom. Limnol. Oceanogr. 43:104-116.
22. Mari, X. 1999. Carbon content and C:N ratio of transparent exopolymeric particles (TEP) produced by bubbling exudates of diatoms. Mar. Ecol. Prog. Ser. 183:59-71.
23. McKee, M. P.J. E. Ward, B. A. MacDonald, and B. A. Holohan. 2005. Production of transparent exopolymer particles by the eastern oyster Crassostrea virginica. Mar. Ecol. Prog. Ser. 183:59-71.
24. Mopper, K., J. A. Zhou, K. S. Ramana, U. Passow, H. G. Dam, and D. T. Drapeau. 1995. The role of surface-active carbohydrates in the flocculation of a diatom bloom in a mesocosm. Deep Sea Res. 42:47-73.
25. Parsons, T. R., Y. Maita, and C. M. Lalli. 1984. A manual of chemical and biological methods for seawater analysis. Pergamon Press.
26. Passow, U. 2000. Formation of transparent exopolymer particles, TEP, from dissolved precursor material. Mar. Ecol. Prog. Ser. 192:1-11.
27. _. 2002a. Transparent exopolymer particles (TEP) in aquatic environments. Prog. Oceanogr. 55:287-333.
28. _. 2002b. Production of transparent exopolymer particles (TEP) by phyto- and bacterioplankton. Mar. Ecol. Prog. Ser. 236:1-12.
29. _ and A. L. Alldredge. 1994. Distribution, size and bacterial colonization of transparent exopolymer particles (TEP) in the ocean. Mar. Ecol. Prog. Ser. 113:185-198.
30. _, _, and B. E. Logan. 1994. The role of particulate carbohydrate exudates in the flocculation of diatom blooms. Deep Sea Res. 41:335-357.
31. _ and _. 1995. A dye-binding assay for the spectrophotometric measurement of transparent exopolymer particles (TEP). Limnol. Oceanogr. 40:1326-1335.
32. 234Th complexation to marine organic matter. Limnol. Oceanogr. 47:367-377.
33. Ramaiah, N., T. Yoshikawa, and K. Furuya. 2001. Temporal variations in transparent exopolymer particles (TEP) associated with a spring diatom bloom in a subarctic ria in Japan. Mar. Ecol. Prog. Ser. 212:79-88.
34. Ramus, J. 1977. Alcian blue: a quantitative aqueous assay for algal acid and sulphated polysaccharides. J. Phycol. 13: 345-348.
35. 234Th and POC export fluxes by marine organisms. Geophys. Res. Lett. 30:1044.
36. Schuster, S., and G. J. Herndl. 1995. Formation and significance of transparent exopolymer particles in the Northern Adriatic Sea. Mar. Ecol. Prog. Ser. 124:227-236.
37. Scott J. E., G. Quintarelli, and M. C. Dellovo. 1964. The chemical and histochemical properties of alcian blue I. the mechanisms of alcian blue staining. Histochemie 4:73-85.
38. Thornton, D. C. O. 2002. Diatom aggregation in the sea: mechanisms and ecological implications. Eur. J. Phycol. 37:149-161.
39. _. 2004. Formation of transparent exopolymeric particles (TEP) from macroalgal detritus. Mar. Ecol. Prog. Ser. 282:1-12.
40. _ and B. Thake. 1998. Effect of temperature on the aggregation of Skeletonema costatum (Bacillariophyceae) and the implication for carbon flux in coastal waters. Mar. Ecol. Prog. Ser. 174:223-237.
41. Underwood, G. J. C., M. Boulcott, C. A. Raines, and K. Waldron. 2004. Environmental effects on exopolymer production by marine benthic diatoms-dynamics, changes in composition and pathways of production. J. Phycol. 40:293-304.
42. Verdugo, P., A. L. Alldredge, F. Azam, D. L. Kirchman, U. Passow, and P. H. Santschi. 2004. The oceanic gel phase: a bridge in the DOM-POM continuum. Mar. Chem. 92:67-85.
43. Worm, J., and M. Søndergaard. 1998. Alcian blue-stained particles in a eutrophic lake. J. Plankton Res. 20:179-186.
44. Wustman, B. A., M. R. Gretz, and K. D. Hoagland. 1997. Extra-cellular matrix assembly in diatoms (Bacillariophyceae). A model of adhesives based on chemical characterization and localization of polysaccharides from the marine diatom Achnanthes longipes and other diatoms. Plant Physiol. 113:1059-1069.
45. Zar, J. H. 1996. Biostatistical analysis, 3rd ed. Prentice Hall International.