Microphytobenthic biomass assessment by pigment analysis: Comparison of spectrophotometry and High Performance Liquid Chromatography methods

Hydrobiologia

Volumn 587, Issue 1, 2007, Pages 19-24

  Brotas, Vanda ^a   Mendes, Carlos Rafel ^a   Cartaxana, Paulo ^a  

^a UNIVERSITY OF LISBON   (Portugal)

Author keywords

Biomass; Chlorophyll a; HPLC; Microphytobenthos; Spectrophotometry; Water content

Indexed keywords

BIOMASS; ESTUARIES; PHOTOSYNTHESIS; PIGMENTS; SPECTROPHOTOMETRY; WATER CONTENT;

CHLOROPHYLL A; HPLC; MICROPHYTOBENTHOS; POSITIVE CORRELATION; ROUTINE ANALYSIS;

LIQUID CHROMATOGRAPHY;

ASSESSMENT METHOD; BIOMASS; CHLOROPHYLL A; COMPARATIVE STUDY; CONCENTRATION (COMPOSITION); LIQUID CHROMATOGRAPHY; PHYTOBENTHOS; PIGMENT; SPECTROPHOTOMETRY; WATER CONTENT;

EURASIA; EUROPE; LISBOA [PORTUGAL]; PORTUGAL; SOUTHERN EUROPE; TAGUS ESTUARY;

EID: 34250747875     PISSN: 00188158     EISSN: 15735117     Source Type: Journal    
DOI: 10.1007/s10750-007-0680-z     Document Type: Conference Paper

References (25)

1. Barlow, R. G., Y. Collos, S. Y. Maestrini & S. Roy, 1990. Microphytobenthic pigments in a salt marsh pond determined by HPLC and spectrophotometry. Marine Microbial Food Webs 4: 117-128.
2. Brotas, V., T. Cabrita, A. Portugal, J. Serôdio & F. Catarino, 1995. Spatio-temporal distribution of the microphytobenthic biomass in intertidal flats of Tagus estuary (Portugal). Hydrobiologia 300-301: 93-104.
3. Brotas, V. & M. R. Plante-Cuny, 1996. Identification et quantification des pigments chlorophylliens et caroténoïdes des sédiments marins: un protocole d'analyse par HPLC. Oceanologica Acta 19: 623-634.
4. Brotas, V. & M. R. Plante-Cuny, 1998. Spatial and temporal patterns of microphytobenthic taxa of estuarine tidal flats in the Tagus estuary (Portugal) using pigment analysis by HPLC. Marine Ecology Progress Series 171: 43-57.
5. Brotas, V. & M. R. Plante-Cuny, 2003. The use of HPLC pigment analysis to study microphytobenthos communities. Acta Oecologica 24: S109-S115.
6. Cahoon, L. B., J. E. Nearhoof & C. L. Tilton, 1999. Sediment grain size effect on benthic microalgal biomass in shallow aquatic ecosystems. Estuaries 22: 735-741.
7. Cartaxana, P. & V. Brotas, 2003. Effects of extraction on HPLC quantification of major pigments from benthic microalgae. Archiv für Hydrobiologie 157: 339-349.
8. Colijn, F. & K. S. Dijkema, 1981. Species composition of benthic diatoms and distribution of chlorophyll a on an intertidal flat in the Dutch Wadden Sea. Marine Ecology Progress Series 4: 9-21.
9. Daemen, E. A. M. J., 1986. Comparison of methods for the determination of chlorophyll in estuarine sediments. Netherlands Journal of Sea Research 20: 21-28.
10. De Jonge, D. J., 1992. Physical Processes and dynamics of microphytobenthos in the Ems estuary (The Netherlands). [PhD Thesis]. University of Groningen, Groningen.
11. Folk, R. L., 1954. The distinction between grain size and mineral composition in sedimentary-rock nomenclature. Journal of Geology 62: 344-359.
12. Honeywill, C., D. M. Paterson & S. E. Hagerthey, 2002. Determination of microphytobenthic biomass using pulse-amplitude modulated minimum fluorescence. European Journal of Phycology 37: 485-492.
13. 2 in higher plants, algae and natural phytoplankton. Biochemical Physiological Pflanzen 167: 191-194.
14. 3 of marine phytoplankton by reversed-phase-C18-high-performance liquid chromatography. Journal of Phycology 28: 708-712.
15. Kromkamp, J. C., E. P. Morris, R. M. Forster, C. Honeywill, S. Hagerthey & D. M. Paterson, 2006. Optical measures of intertidal sediments: relationship of surface sediment chlorophyll concentration with hyper-spectral reflectance or chlorophyll fluorescence. Estuaries and Coasts 29: 183-196.
16. Lorenzen, C. J., 1967. Determination of chlorophyll and pheo-pigments: spectrophotometric equations. Limnology and Oceanography 12: 343-346.
17. Lorenzen, C. J. & S. W. Jeffrey, 1980. Determination of chlorophyll in seawater: report of intercalibration tests. UNESCO Technical Papers in Marine Science 35: 1-20.
18. Neveux, J., D. Delmas, J. C. Romano, P. Algarra, L. Ignatiades, A. Herbland, P. Morand, A. Neori, D. Bonin, J. Barbe, A. Sukenik & T. Berman, 1990. Comparison of chlorophyll and phaeopigment determinations by spectrophotometric, fluorometric, spectrofluorometric and HPLC methods. Marine Microbial Food Webs 4: 217-238.
19. Perkins, R. G., C. Honeywill, M. Consalvey, H. A. Austin, T. J. Tolhurst & D. M. Paterson, 2003. Changes in microphytobenthic chlorophyll a and EPS resulting from sediment compaction due to de-watering: opposing patterns in concentration and content. Continental Shelf Research 23: 575-586.
20. Pinckney, J., R. Papa & R. Zingmark, 1994. Comparison of high-performance liquid chromatographic, spectrophotometric, and fluorometric methods for determining chlorophyll a concentrations in estuarine sediments. Journal of Microbiological Methods 19: 59-66.
21. Plante-Cuny, M. R., C. Barranguet, D. Bonin & C. Grenz, 1993. Does chlorophyllide a reduce reliability of chlorophyll a measurements in marine coastal sediments? Aquatic Science 55: 19-30.
22. Riaux-Gobin, C. & P. Bourgoin, 2002. Microphytobenthos biomass at Kerguelen's Land (Subantarctic Indian Ocean): repartition variability during austral summers. Journal of Marine Systems 32: 295-306.
23. Riaux-Gobin, C., C. A. Llewellyn & B. Klein, 1987. Microphytobenthos from two subtidal sediments from North Brittany. II. Variations of pigment compositions and concentrations determined by HPLC and conventional techniques. Marine Ecology Progress Series 40: 275-283.
24. Underwood, G. F. C. & J. Kromkamp, 1999. Primary production by phytoplankton and microphytobenthos in estuaries. Advances in Ecological Research 29: 93-153.
25. Varela, M., 1981. El problema de la determinación de clorofila a en el fitomicrobentos: discusión sobre la metodología. Oecologia Aquatica 5: 7-19.