miR-15b and miR-16 are implicated in activation of the rat hepatic stellate cell: An essential role for apoptosis

Journal of Hepatology

Volumn 50, Issue 4, 2009, Pages 766-778

  Guo, Can Jie ^a   Pan, Qin ^a   Li, Ding Guo ^a   Sun, Hua ^a   Liu, Bo Wei ^a  

^a School of Medicine   (China)

Author keywords

Activation; Apoptosis; Bcl 2; Caspase; Gene ontology; Hepatic stellate cells; miR 15b; miR 16

Indexed keywords

CASPASE 3; CASPASE 8; CASPASE 9; LIPOCORTIN 5; MICRORNA; PROTEIN BCL 2;

ANIMAL CELL; APOPTOSIS; ARTICLE; CELL ACTIVATION; CONTROLLED STUDY; DOWN REGULATION; FLOW CYTOMETRY; GENE EXPRESSION PROFILING; GENETIC ANALYSIS; LIVER CELL; MALE; MICROARRAY ANALYSIS; NICK END LABELING; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RAT; REAL TIME POLYMERASE CHAIN REACTION; STELLATE CELL; UPREGULATION;

ACTINS; ANIMALS; APOPTOSIS; DESMIN; DNA PRIMERS; DOWN-REGULATION; FLOW CYTOMETRY; HEPATIC STELLATE CELLS; IN SITU NICK-END LABELING; MALE; MICROARRAY ANALYSIS; MICRORNAS; RATS; RATS, SPRAGUE-DAWLEY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; UP-REGULATION;

EID: 61849096150     PISSN: 01688278     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jhep.2008.11.025     Document Type: Article

References (27)

1. Aleffi S., Petrai I., Bertolani C., Parola M., Colombatto S., Novo E., et al. Upregulation of proinflammatory and proangiogenic cytokines by leptin in human hepatic stellate cells. Hepatology 42 (2005) 1339-1348
2. Friedman S.L. Mechanisms of disease: mechanisms of hepatic fibrosis and therapeutic implications. Nat Clin Pract Gastroenterol Hepatol 1 (2004) 98-105
3. Kruglov E.A., Correa P.R., Arora G., Yu J., Nathanson M.H., and Dranoff J.A. Molecular basis for calcium signaling in hepatic stellate cells. Am J Physiol Gastrointest Liver Physiol 292 (2007) 975-982
4. Jiang F., Parsons C.J., and Stefanovic B. Gene expression profile of quiescent and activated rat hepatic stellate cells implicates Wnt signaling pathway in activation. J Hepatol 45 (2006) 401-409
5. Chitwood D.H., and Timmermans M.C. Target mimics modulate miRNAs. Nat Genet 39 (2007) 935-936
6. Ambros V. The functions of animal microRNAs. Nature 431 (2004) 350-355
7. Chen C.Z., Li L., Lodish H.F., and Bartel D.P. MicroRNAs modulate hematopoietic lineage differentiation. Science 303 (2004) 83-86
8. Roldo C., Missiaglia E., Hagan J.P., Falconi M., Capelli P., Bersani S., et al. MicroRNA expression abnormalities in pancreatic endocrine and acinar tumors are associated with distinctive pathologic features and clinical behavior. J Clin Oncol 24 (2006) 4677-4684
9. Zhao J.J., Hua Y.J., Sun D.G., Meng X.X., Xiao H.S., and Ma X. Genome-wide microRNA profiling in human fetal nervous tissues by oligonucleotide microarray. Childs Nerv Syst 22 (2006) 1419-1425
10. Friedman S.L., and Roll F.J. Isolation and culture of hepatic lipocytes, Kupffer cells, and sinusoidal endothelial cells by density gradient centrifugation with Stractan. Anal Biochem 161 (1987) 207-218
11. Ying-Bin H., Ding-Guo L., and Han-Ming L. Modified synthetic siRNA targeting tissue inhibitor of metalloproteinase-2 inhibits hepatic fibrogenesis in rats. J Gene Med 9 (2007) 217-229
12. Cassiman D., Denef C., Desmet V., and Roskams T. Human and rat hepatic stellate cells express neurotrophins and neurotrophin receptors. Hepatology 33 (2001) 148-158
13. Chen C., Ridzon D.A., Broomer A.J., Zhou Z., Lee D.H., Nguyen J.T., et al. Real-time quantification of microRNAs by stem-loop RT-PCR. Nucleic Acids Res 33 (2005) e179
14. Livak K.J., and Schmittgen T.D. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T))method. Methods 25 (2001) 402-408
15. Gene Ontology Consortium. The Gene Ontology (GO) project in 2006. Nucleic Acids Res 2006;34:322-326.
16. Lim L.P., Lau N.C., Garrett-Engele P., Grimson A., Schelter J.M., Castle J., et al. Microarray analysis shows that some microRNAs downregulate large numbers of target mRNAs. Nature 433 (2005) 769-773
17. Jiang P., Yan P.K., Chen J.X., Zhu B.Y., Lei X.Y., and Yin W.D. High density lipoprotein 3 inhibits oxidized low density lipoproteininduced apoptosis via promoting cholesterol efflux in RAW264.7 cells. Acta Pharmacologica Sinica 27 (2006) 151-157
18. Gold R., Schmied M., Giegerich G., Breitschopf H., Hartung H.P., and Toyka K.V. Differentiation between cellular apoptosis and necrosis by the combined use of in situ tailing and nick translation techniques. Lab Invest 71 (1994) 219-225
19. Boersma A.W., Nooter K., Oostrum R.G., and Stoter G. Quantification of apoptotic cells with fluorescein isothiocyanate-labeled annexin V in chinese hamster ovary cell cultures treated with cisplatin. Cytometry 24 (1996) 123-130
20. Inagaki Y., Kushida M., Higashi K., Itoh J., Higashiyama R., Hong Y.Y., et al. Cell type-specific intervention of transforming growth factor beta/Smad signaling suppresses collagen gene expression and hepatic fibrosis in mice. Gastroenterology 129 (2005) 259-268
21. Lee J.I., Lee K.S., Paik Y.H., Nyun Park Y., Han K.H., Chon C.Y., et al. Apoptosis of hepatic stellate cells in carbon tetrachloride induced acute liver injury of the rat: analysis of isolated hepatic stellate cells. J Hepatol 39 (2003) 960-966
22. Schnabl B., Purbeck C.A., Choi Y.H., Hagedorn C.H., and Brenner D. Replicative senescence of activated human hepatic stellate cells is accompanied by a pronounced inflammatory but less fibrogenic phenotype. Hepatology 37 (2003) 653-664
23. Novo E., Marra F., Zamara E., Valfrè di Bonzo L., Monitillo L., Cannito S., et al. Overexpression of Bcl-2 by activated human hepatic stellate cells: resistance to apoptosis as a mechanism of progressive hepatic fibrogenesis in humans. Gut 55 (2006) 1174-1182
24. Kawada N. Human hepatic stellate cells are resistant to apoptosis: implications for human fibrogenic liver disease. Gut 55 (2006) 1073-1074
25. Cimmino A., Calin G.A., Fabbri M., Iorio M.V., Ferracin M., and Shimizu M. miR-15 and miR-16 induce apoptosis by targeting BCL2. Proc Natl Acad Sci USA 102 (2005) 13944-13949
26. Xia L., Zhang D., Du R., Pan Y., Zhao L., Sun S., et al. miR-15b and miR-16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells. Int J Cancer 123 (2008) 372-379
27. Wang X.Z., Zhang S.J., Chen Y.X., Chen Z.X., Huang Y.H., and Zhang L.J. Effects of platelet-derived growth factor and interleukin-10 on Fas/Fas-ligand and Bcl-2/Bax mRNA expression in rat hepatic stellate cells in vitro. World J Gastroenterol 10 (2004) 2706-2710