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Volumn , Issue 10, 2009, Pages 1225-1227

Strand invasion of conventional PNA to arbitrary sequence in DNA assisted by single-stranded DNA binding protein

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EID: 60849110279     PISSN: 13597345     EISSN: None     Source Type: Journal    
DOI: 10.1039/b813975j     Document Type: Article
Times cited : (12)

References (24)
  • 19
    • 0016609346 scopus 로고
    • SSB was previously used to facilitate the analysis of target DNA using combination of PNA opener (ref. 3) and polymerase, in which SSB promoted strand-displacement by the polymerase;
    • J. Weiner L. Bertsch A. Kornberg J. Biol. Chem. 1975 250 1972 1980
    • (1975) J. Biol. Chem. , vol.250 , pp. 1972-1980
    • Weiner, J.1    Bertsch, L.2    Kornberg, A.3
  • 21
    • 0037432326 scopus 로고    scopus 로고
    • The PNA strands bearing l-lysine residue at its C- and N-terminus were synthesized by Boc-chemistry, purified by the reversed-phase HPLC, and characterized by MALDI-TOF MS (Table S1 and Fig. S1, ESI). The target 203-mer dsDNA was prepared by PCR from pBR322 plasmid (C1724-G1926, Fig. S2, ESI). Gel-shift assay was achieved by 5%-nondenaturing polyacrylamide gel electrophoresis in TBE buffer at 25°C The weak band below the invasion complex in lane 4 (and lane 7) of Fig. 2 is associated with a non-specific complex between the dsDNA and SSB which is formed in equilibrium in the solutions. Consistently, this band is also observed in lane 3 where only dsDNA and SSB are mixed
    • H. B. Gamper C. J. Nulf D. R. Corey E. B. Kmiec Biochemistry 2003 42 2643 2655
    • (2003) Biochemistry , vol.42 , pp. 2643-2655
    • Gamper, H.B.1    Nulf, C.J.2    Corey, D.R.3    Kmiec, E.B.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.