Strand displacement binding of a duplex-forming homopurine PNA to a homopyrimidine duplex DNA target

Journal of the American Chemical Society

Volumn 118, Issue 9, 1996, Pages 2287-2288

  Nielsen, Peter E ^a   Christensen, Leif ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

DOUBLE STRANDED DNA; NUCLEIC ACID; PEPTIDE DERIVATIVE;

ARTICLE; DNA BINDING;

EID: 0029963160     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja953125q     Document Type: Article

References (23)

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2. Dervan, P. B. Nature 1992, 359, 87-88. Hélène, C. Curr. Opin. Biotechnol. 1993, 4, 29-36. Frank-Kamentskii, M. D.; Mirkin, S. M. Annu. Rev. Biochem. 1995, 64, 65-95.
3. Dervan, P. B. Nature 1992, 359, 87-88. Hélène, C. Curr. Opin. Biotechnol. 1993, 4, 29-36. Frank-Kamentskii, M. D.; Mirkin, S. M. Annu. Rev. Biochem. 1995, 64, 65-95.
4. (a) Nielsen, P. E.; Egholm, M.; Berg, R. H.; Buchardt, O. Science 1991, 254, 1497-1500.
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14. PNAs were synthesized according to the following: Dueholm, K. L.; Egholm, M.; Behrens, C.; Christensen, L.; Hansen, H. F.; Vulpius, T.; Petersen, K.; Berg, R. H.; Nielsen, P. E.; Buchardt, O. J. Org. Chem. 1994, 59, 5767-5773. Christensen, L.; Fitzpatrick, R.; Gildea, B.; Petersen, K. H.; Hansen, H. F.; Koch, T.; Egholm, M.; Buchardt, O.; Nielsen, P. E.; Coull, J.; Berg, R. H. J. Pept. Sci. 1995, 3, 175-183.
15. PNAs were synthesized according to the following: Dueholm, K. L.; Egholm, M.; Behrens, C.; Christensen, L.; Hansen, H. F.; Vulpius, T.; Petersen, K.; Berg, R. H.; Nielsen, P. E.; Buchardt, O. J. Org. Chem. 1994, 59, 5767-5773. Christensen, L.; Fitzpatrick, R.; Gildea, B.; Petersen, K. H.; Hansen, H. F.; Koch, T.; Egholm, M.; Buchardt, O.; Nielsen, P. E.; Coull, J.; Berg, R. H. J. Pept. Sci. 1995, 3, 175-183.
16. An analysis of seven PNA-DNA decamer complexes indicates the thermal stability of these (to a first approximation) to be linearly correlated to the purine content of the PNA strand, and not, as seen for DNA-DNA duplexes, directly correlated to the total G-C content.
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22. 2 triplex binding to the target.
23. At higher PNA concentrations both the diethyl pyrocarbonate and the permanganate hypersensitivity extended partly through the short (30 base pairs) and thus easily denaturable end of the DNA fragment (Figure 2, Ianes 7 and 15). We ascribe this to unspecific PNA binding induced by the target-specific PNA binding.