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Volumn 81, Issue 2, 2009, Pages 699-704

Segregation of molecules in lipid bilayer spreading through metal nanogates

Author keywords

[No Author keywords available]

Indexed keywords

DEVICE TECHNOLOGIES; EXTERNAL FIELDS; FILTERING EFFECTS; MOLECULAR TRANSPORTS; NANO GATES; PERIODIC ARRAYS; QUANTITATIVE ANALYSIS; SELF-SPREADING; SPREADING DYNAMICS; STRUCTURAL CHANGES;

EID: 60549101334     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac802130e     Document Type: Article
Times cited : (27)

References (38)
  • 1
  • 24
    • 64349106691 scopus 로고    scopus 로고
    • For quantitative intensity analysis, at least 50 line profiles were averaged on each channel. The smoothing process, by which three data points are averaged with their neighbors, was done for each averaged profile. After the averaging and smoothing procedures, three additional calibrations were performed. The first of these was a background calibration, in which the background intensity was simply subtracted from the profile. The second was an area calibration, in which an underestimation in the fluorescence intensity due to the presence of nonwettable gold architecture, which is involved during the averaging processes, was calibrated by dividing the profile by an area fraction of bare glass region. The area fractions were 0.833 (CH500, 0.792 (CH200, 0.773 (CH100, and 0.767 CH75, The last was a calibration for photobleaching. The intensity lowering due to the photobleaching is time-dependent. The line profiles were calibrated by the calibration curve between fluorescence intensity
    • For quantitative intensity analysis, at least 50 line profiles were averaged on each channel. The smoothing process, by which three data points are averaged with their neighbors, was done for each averaged profile. After the averaging and smoothing procedures, three additional calibrations were performed. The first of these was a background calibration, in which the background intensity was simply subtracted from the profile. The second was an area calibration, in which an underestimation in the fluorescence intensity due to the presence of nonwettable gold architecture, which is involved during the averaging processes, was calibrated by dividing the profile by an area fraction of bare glass region. The area fractions were 0.833 (CH500), 0.792 (CH200), 0.773 (CH100), and 0.767 (CH75). The last was a calibration for photobleaching. The intensity lowering due to the photobleaching is time-dependent. The line profiles were calibrated by the calibration curve between fluorescence intensity and photoirradiation time that was acquired using of supported membrane.
  • 26
    • 64349086114 scopus 로고    scopus 로고
    • gate × 100 molecules.
    • gate × 100 molecules.


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.