Universal and rapid method for purification of GFP-like proteins by the ethanol extraction

Protein Expression and Purification

Volumn 65, Issue 1, 2009, Pages 108-113

  Samarkina, Olga N ^a   Popova, Anastasia G ^a   Gvozdik, Elena Yu ^a   Chkalina, Anna V ^a   Zvyagin, Ivan V ^a   Rylova, Yulia V ^a   Rudenko, Natalia V ^a   Lusta, Konstantin A ^a   Kelmanson, Ilya V ^b   Gorokhovatsky, Andrey Yu ^a   Vinokurov, Leonid M ^a  

^a SHEMYAKIN OVCHINNIKOV INSTITUTE OF BIOORGANIC CHEMISTRY   (Russian Federation)

^b Evrogen   (Russian Federation)

Author keywords

Absorption spectra; Butyl Sepharose chromatography; Chromophore maturation; GFP like fluorescent proteins; Green fluorescent protein; Organic extraction; Recombinant protein expression and purification; Two phase water organic system

Indexed keywords

ALCOHOL; GREEN FLUORESCENT PROTEIN; RECOMBINANT PROTEIN;

ANIMAL; ARTICLE; BIOSYNTHESIS; CHEMISTRY; ISOLATION AND PURIFICATION;

ANIMALS; ETHANOL; GREEN FLUORESCENT PROTEINS; RECOMBINANT PROTEINS;

ESCHERICHIA COLI;

EID: 60349121424     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.11.008     Document Type: Article

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