Evaluation of recombinant green fluorescent protein, under various culture conditions and purification with HiTrap hydrophobic interaction chromatography resins

Applied Biochemistry and Biotechnology - Part A Enzyme Engineering and Biotechnology

Volumn 114, Issue 1-3, 2004, Pages 453-468

  Penna, Thereza Christina Vessoni ^a   Ishii, Marina ^a   Junior, Adalberto Pessoa ^a   Nascimento, Laura De Oliveira ^a   De Souza, Luciana Cambricoli ^a   Cholewa, Olivia ^b  

^a UNIVERSITY OF SÃO PAULO   (Brazil)

^b MOLECULAR PROBES INC   (United States)

Author keywords

GFPuv; Hydrophobic interaction chromatography; Recombinant green fluorescent protein; Sodium dodecylsulfate polyacrylamide gel electrophoresis; Three phase partitioning extraction

Indexed keywords

CELLS; CHROMATOGRAPHY; ESCHERICHIA COLI; FLUORESCENCE; MOLECULAR WEIGHT; RESINS;

GREEN FLUORESCENT PROTEINS; PROTEIN EXTRACTS; THREE-PHASE PARTITIONING (TPP);

PROTEINS;

DODECYL SULFATE SODIUM; GREEN FLUORESCENT PROTEIN; RECOMBINANT PROTEIN; RESIN; ISOPROPYL THIOGALACTOSIDE; PHOTOPROTEIN; PROTEIN;

ANIMAL CELL; BACTERIAL GROWTH; CONFERENCE PAPER; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; BIOTECHNOLOGY; CHEMISTRY; CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PH; PLASMID; TEMPERATURE; TIME;

ANIMALIA; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

ANIMALS; BIOTECHNOLOGY; CHROMATOGRAPHY; CHROMATOGRAPHY, AFFINITY; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GREEN FLUORESCENT PROTEINS; HYDROGEN-ION CONCENTRATION; ISOPROPYL THIOGALACTOSIDE; LUMINESCENT PROTEINS; PLASMIDS; PROTEINS; RECOMBINANT PROTEINS; TEMPERATURE; TIME FACTORS;

EID: 11244308944     PISSN: 02732289     EISSN: None     Source Type: Journal    
DOI: 10.1385/ABAB:114:1-3:453     Document Type: Conference Paper

References (11)

1. Chalfie, M. and Kain, S. (1998), in Green Fluorescent Protein Properties: Applications and Protocols. Chalfie, M. and Kain, S., eds., Wiley-Liss, New York, NY, pp. 3-75.
2. Wheelwright, S. M. (1991), Protein Purification, Design and Scale Up of Downstream Processing. Wheelwright, S. M., ed., John Wiley & Sons, New York, NY, pp. 154-162.
3. nd Ed., Nolan, C., ed., Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, pp. 1.74-1.84.
4. Vessoni Penna, T. C., Chiarini, E., Machoshvili, I. A., Ishii, M., and Pessoa Jr., A. (2002), Appl. Biochem. Biotechnol. 98-100, 791-802.
5. Denninson, C., and Lovrien, R. (1997), Protein Expr. Purif. 11, 149-161.
6. Sharma, A. and Gupta, M. N. (2001), Process Biochem. 37, 193-196.
7. Vessoni Penna, T. C., Chiarini, E., and Pessoa Jr., A. (2003), Appl. Biochem. Biotechnol. 105-108, 481-491.
8. Drapper, N. and Smith, H. (1981), Applied Regression Analysis. John Wiley, New York, NY.
9. rd Ed., Cantor, C. R., ed., Springer-Verlag, New York, NY, pp. 39-89, 324-325.
10. Shi, H. and Su, W. W. (2001), Enzyme Microb. Technol. 28, 25-34.
11. Yakhnin, A. V., Vinokurov, L. M., Surin, A. K., and Alalhov, Y. B. (1998), Protein Expr. Purif. 14, 382-386.