Modeling Pseudomonas aeruginosa pathogenesis in plant hosts

Nature Protocols

Volumn 4, Issue 2, 2009, Pages 117-124

  Starkey, Melissa ^a,b,c   Rahme, Laurence G ^a,b,c  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c Shriners Burns Institute   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

VIRULENCE FACTOR;

ARABIDOPSIS; ARTICLE; BACTERIAL GROWTH; BACTERIAL PLANT DISEASE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; LETTUCE; NONHUMAN; PLANT LEAF; PLANT PATHOGEN INTERACTION; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; QUALITATIVE ANALYSIS; QUANTITATIVE ANALYSIS;

ANIMALS; ARABIDOPSIS; LETTUCE; MICE; PLANT DISEASES; PLANT LEAVES; PSEUDOMONAS AERUGINOSA; VIRULENCE;

ARABIDOPSIS; BACTERIA (MICROORGANISMS); LACTUCA; PSEUDOMONAS AERUGINOSA;

EID: 59849121929     PISSN: 17542189     EISSN: 17502799     Source Type: Journal    
DOI: 10.1038/nprot.2008.224     Document Type: Article

References (38)

1. Rahme, L.G. et al. Common virulence factors for bacterial pathogenicity in plants and animals. Science 268, 1899-1902 (1995).
2. Nordmann, P., Naas, T., Fortineau, N. & Poirel, L. Superbugs in the coming new decade; multidrug resistance and prospects for treatment of Staphylococcus aureus, Enterococcus spp. and Pseudomonas aeruginosa in 2010. Curr. Opin. Microbiol. 10, 436-440 (2007).
3. Ausubel, F.M. Are innate immune signaling pathways in plants and animals conserved? Nat. Immunol. 6, 973-979 (2005).
4. Rahme, L.G. et al. Common virulence factors for bacterial pathogenicity in plants and animals. Science 268, 1899-1902 (1995).
5. Apidianakis, Y. et al. Involvement of skeletal muscle gene regulatory network in susceptibility to wound infection following trauma. PLoS ONE 2, e1356 (2007).
6. Apidianakis, Y. et al. Profiling early infection responses: Pseudomonas aeruginosa eludes host defenses by suppressing antimicrobial peptide gene expression. Proc. Natl. Acad. Sci. USA 102, 2573-2578 (2005).
7. Mahajan-Miklos, S., Tan, M.-W., Rahme, L.G. & Ausubel, F.M. Molecular mechanisms of bacterial virulence elucidated using a Pseudomonas aeruginosa-Caenorhabditis elegans pathogenesis model. Cell 96, 47-56 (1999).
8. Cosson, P. et al. Pseudomonas aeruginosa virulence analyzed in a Dictyostelium discoideum host system. J. Bacteriol. 184 3027-3033 (2002).
9. Mahajan-Miklos, S., Rahme, L.G. & Ausubel, F.M. Elucidating the molecular mechanisms of bacterial virulence using non-mammalian hosts. Mol. Microbiol. 37, 981-988 (2000).
10. Lau, G.W. et al. The Drosophila melanogaster toll pathway participates in resistance to infection by the gram-negative human pathogen Pseudomonas aeruginosa. Infect. Immun. 71, 4059-4066 (2003).
11. Hoffmann, J.A. The immune response of Drosophila. Nature 426 33-38 (2003).
12. Hoffmann, J.A. et al. Phylogenetic perspectives in innate immunity. Science 284, 1313-1318 (1999).
13. Shan, L., He, P. & Sheen, J. Intercepting host MAPK signaling cascades by bacterial type III effectors. Cell Host Microbe 1, 167-174 (2007).
14. Apostol, I., Heinstein, P.F. & Low, P.S. Rapid stimulation of an oxidative burst during elicitation of cultured plant cells: Role in defense and signal transduction. Plant Physiol. 90, 109-116 (1989).
15. 2-generation in membrane fractions isolated from wounded potato tubers inoculated with Phytophthora infestans. Physiol. Plant Pathol. 27, 311-322 (1985).
16. Ohashi, Y., Brickman, J.M., Furman, E., Middleton, B. & Carey, M. Modulating the potency of an activator in a yeast in vitro transcription system. Mol. Cell Biol. 14, 2731-2739 (1994).
17. Sutherland, M.W. The generation of oxygen radicals during host plant responses to infection. Physiol. Mol. Plant Pathol. 39, 79-94 (1991).
18. Hunter, P. Common defences. EMBO Rep. 6, 504-507 (2005).
19. Broekaert, W.F., Terras, F.R., Cammue, B.P. & Osborn, R.W. Plant defensins: Novel antimicrobial peptides as components of the host defense system. Plant Physiol. 108, 1353-1358 (1995).
20. Ganz, T. & Lehrer, R.I. Defensins. Curr. Opin. Immunol. 6, 584-589 (1994).
21. van Baarlen, P., van Belkum, A. & Thomma, B.P.H.J. Disease induction by human microbial pathogens in plant-model systems: Potential, problems and prospects. Drug Discov. Today 12, 167-173 (2007).
22. Jha, A.K., Bais, H.P. & Vivanco, J.M. Enterococcus faecalis mammalian virulence-related factors exhibit potent pathogenicity in the Arabidopsis thaliana plant model. Infect Immun. 73, 464-475 (2005).
23. Prithiviraj, B., Bais, H.P., Jha, A.K. & Vivanco, J.M. Staphylococcus aureus pathogenicity on Arabidopsis thaliana is mediated either by a direct effect of salicylic acid on the pathogen or by SA-dependent, NPR1-independent host responses. Plant J. 42, 417-432 (2005).
24. Rahme, L.G. et al. Use of model plant hosts to identify Pseudomonas aeruginosa virulence factors. Proc. Natl. Acad. Sci. USA 94, 13245-13250 (1997).
25. Rahme, L.G. et al. Plants and animals share functionally common bacterial virulence factors. Proc. Natl. Acad. Sci. USA 97, 8815-8821 (2000).
26. Dong, X., Mindrinos, M., Davis, K.R. & Ausubel, F.M. Induction of Arabidopsis defense genes by virulent and Avirulent Pseudomonas syringae strains and by a cloned Avirulence gene. Plant Cell 3, 61-72 (1991).
27. Katagiri, F., Thilmony, R. & He, S.Y. The Arabidopsis thaliana-Pseudomonas syringae interaction. The Arabidopsis Book Rockville, MD: American Society of Plant Biologists. DOI: 10.1199/tab.0039 (2002).
28. Plotnikova, J.M., Rahme, L.G. & Ausubel, F.M. Pathogenesis of the human opportunistic pathogen Pseudomonas aeruginosa PA14 in Arabidopsis. Plant Physiol. 124, 1766-1774 (2000).
29. Walker, T.S. et al. Pseudomonas aeruginosa-plant root interactions. Pathogenicity, biofilm formation, and root exudation. Plant Physiol. 134, 320-331 (2004).
30. Baldini, R.L., Lau, G.W. & Rahme, L.G. Use of plant and insect hosts to model bacterial pathogenesis. Methods Enzymol. 358, 3-13 (2002).
31. Silo-Suh, L., Suh, S.-J., Sokol, P.A. & Ohman, D.E. A simple alfalfa seedling infection model for Pseudomonas aeruginosa strains associated with cystic fibrosis shows AlgT (sigma-22) and RhlR contribute to pathogenesis. Proc. Natl. Acad. Sci. USA 99, 15699-15704 (2002).
32. Attila, C. et al. Pseudomonas aeruginosa PA01 virulence factors and poplar tree response in the rhizosphere. Microbial Biotechnol. 1, 17-29 (2008).
33. Kaplan, E. & Meier, P. Nonparametric estimation from incomplete observations. J. Am. Stat. Assoc. 53, 457-481 562-563 (1958).
34. Jander, G., Rahme, L.G. & Ausubel, F.M. Positive correlation between virulence of Pseudomonas aeruginosa mutants in mice and insects. J. Bacteriol. 182, 3843-3845 (2000).
35. Miyata, S., Casey, M., Frank, D.W., Ausubel, F.M. & Drenkard, E. Use of the Galleria mellonella caterpillar as am odel host to study the role of the type III secretion system in Pseudomonas aeruginosa pathogenesis. Infect. Immun. 71, 2404-2413 (2003).
36. Fauvarque, M.O. et al. Role and activation of type III secretion system genes in Pseudomonas aeruginosa-induced Drosophila killing. Microb. Pathog. 32, 287-295 (2002).
37. Liberati, N.T. et al. An ordered, nonredundant library of Pseudomonas aeruginosa strain PA14 transposon insertion mutants. Proc. Natl. Acad. Sci. USA 103, 2833-2838 (2006).
38. Jacobs, M.A. et al. Comprehensive transposon mutant library of Pseudomonas aeruginosa. Proc. Natl. Acad. Sci. USA 100, 14339-14344 (2003).