Molecular mechanisms of bacterial virulence elucidated using a Pseudomonas aeruginosa-Caenorhabditis elegans pathogenesis model

Cell

Volumn 96, Issue 1, 1999, Pages 47-56

  Mahajan Miklos, Shalina ^a   Tan, Man Wah ^a,b   Rahme, Laurence G ^a   Ausubel, Frederick M ^a  

^a MASSACHUSETTS GENERAL HOSPITAL   (United States)

^b HARVARD UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTHRANILIC ACID; BACTERIAL DNA; PHENAZINE; PYOCYANINE;

ARTICLE; BACTERIAL MUTATION; BACTERIAL VIRULENCE; CAENORHABDITIS ELEGANS; DNA SEQUENCE; GRAM NEGATIVE INFECTION; HOST RESISTANCE; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; OPPORTUNISTIC INFECTION; OXIDATIVE STRESS; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA;

ANIMALIA; BACTERIA (MICROORGANISMS); CAENORHABDITIS ELEGANS; NEGIBACTERIA; PSEUDOMONAS; PSEUDOMONAS AERUGINOSA;

EID: 0033534534     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0092-8674(00)80958-7     Document Type: Article

References (50)

1. Anderson, D.M., and Mills, D. (1985). The use of transposon mutagenesis in the isolation of nutritional and virulence mutants in two pathovars of Pseudomonas syringae. Phytopath. 75, 104-108.
2. Andrew, P.A., and Nicholas, W.L. (1976). Effect of bacteria on dispersal of Caenorhabditis elegans (Rhabditidae). Nematologica 22, 451-461.
3. Arrigo, A.P. (1998). Small stress proteins: chaperones that act as regulators of intracellular redox state and programmed cell death. Biol. Chem. 379, 19-26.
4. Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., and Struhl, K. (1996). Current Protocols in Molecular Biology (New York: John Wiley and Sons).
5. Brenner, S. (1974). The genetics of Caenorhabditis elegans. Genetics 77, 71-94.
6. Broeks, A., Janssen, H.W., Calafat, J., and Plasterk, R.H. (1995). A P-glycoprotein protects Caenorhabditis elegans against natural toxins. EMBO J. 14, 1858-1866.
7. Camhi, S.L, Lee, P., and Choi, A.M. (1995). The oxidative stress response. New Horizons 3, 170-182.
8. Donnenberg, M.S., and Kaper, J.B. (1991). Construction of an eae deletion mutant of enteropathogenic Escherichia coli by using a positive-selection suicide vector. Infect. Immun. 59, 4310-4317.
9. Doring, D. (1993). Chronic Pseudomonas aeruginosa lung infection in cystic fibrosis patients. In Pseudomonas aeruginosa as an Opportunistic Pathogen, M. Campa, ed. (New York: Plenum Press), pp. 245-273.
10. Essar, D.W., Eberly, L., Hadero, A., and Crawford, I.P. (1990). Identification and characterization of genes for a second anthranilate synthase in Pseudomonas aeruginosa: interchangeability of the two anthranilate synthases and evolutionary implications. J. Bacteriol. 172, 884-900.
11. Gish, W., and States, D.J. (1993). Identification of protein coding regions by database similarity search. Nat. Genet. 3, 266-272.
12. Hartman, P.S., and Herman, R.K. (1982). Radiation-sensitive mutants of Caenorhabditis elegans. Genetics 102, 159-178.
13. Higgins, C.F. (1992). ABC Transporters, from microorganisms to man. Annu. Rev. Cell Biol. 8, 67-113.
14. Higgins, C.F. (1995). The ABC of channel regulation. Cell 82, 693-696.
15. Ishii, N., Takahashi, K., Tomita, S., Keino, T., Honda, S., Yoshino, K., and Suzuki, K. (1990). A methyl viologen-sensitive mutant of the nematode Caenorhabditis elegans. Mutation Res. 237, 165-171.
16. Ishii, N., Suzuki, N., Hartman, P.S., and Suzuki, K. (1993). The radiation-sensitive mutant rad-8 of Caenorhabditis elegansis hypersensitive to the effects of oxygen on aging and development. Mech. Ageing Dev. 68, 1-10.
17. Ishii, N., Fujii, M., Hartman, P.S., Tsuda, M., Yasuda, K., SenooMatsuda, N., Yanase, S., Ayusawa, D., and Suzuki, K. (1998). A mutation in succinate dehydrogenase cytochrome b causes oxidative stress and ageing in nematodes. Nature 394, 694-697.
18. Johnson, T.E. (1990). Increased life-span of age- 7 mutants in Caenorhabditis elegans and lower Gompertz rate of aging. Science 249, 908-912.
19. Kennedy, E.P. (1996). Membrane-derived oligosaccharides (periplasmic β-D-glucans) of Escherichia coli. In Escherichia coli and Salmonella, F.C. Neidhardt, ed. (Washington, D.C.: American Society for Microbiology Press), pp. 1064-1071.
20. Kimura, K.D., Tissenbaum, H.A., Liu, Y., and Ruvkun, G. (1997). daf-2, an insulin-receptor-like gene that regulates longevity and diapause in Caenorhabditis elegans. Science 277, 942-946.
21. King, E.O., Ward, M.K., and Raney, D.E. (1954). Two simple media for the demonstration of phycocyanin and fluorescin. J. Lab. Clin. Med. 44, 301-307.
22. Larsen, P.L (1993). Aging and resistance to oxidative damage in Caenorhabditis elegans. Proc. Natl. Acad. Sci. USA 90, 8905-8909.
23. Li, X.-Z., Nikaido, H., and Poole, K. (1995). Role of MexA-MexB-OpiM in antibiotic efflux in Pseudomonas aeruginosa. Antimicrob. Agents Chemother. 39, 1948-1953.
24. Lindquist, S. (1992). Heat-shock proteins and stress tolerance in microorganisms. Curr. Opin. Genet. Dev. 2, 748-755.
25. Loubens, I., Debarbieux, L, Bohin, A., Lacroix, J.-M., and Bohin, J.-P. (1993). Homology between a genetic locus (mdoA) involved in the osmoregulated biosynthesis of periplasmic glucans in Escherichia coli and a genetic locus (hrpM) controlling pathogenicity of Pseudomonas syringae. Mol. Microbiol. 10, 329-340.
26. Manoil, C., and Beckwith, J. (1985). Tnpho/4: a transposon probe for protein export signals. Proc. Natl. Acad. Sci. USA 82, 8129-8133.
27. Mavrodi, D.V., Ksenzenko, V.N., Bonsall, R.F., Cook, R.J., Boronin, A.M., and Thomashow, L.S. (1998). A seven-gene locus for synthesis of phenazine-1-carboxylic acid by Pseudomonas fluorescens 2-79. J. Bacteriol. 180, 2541-2548.
28. Medzhitov, R., and Janeway, C.A., Jr. (1998). An ancient system of host defense. Curr. Opin. Immunol. 10, 12-15.
29. Miller, J.H. (1972). Experiments in Molecular Genetics, 2nd Edition (Cold Spring Harbor, New York: Cold Spring Harbor Laboratory).
30. Miller, J.F., Mekalanos, J.J., and Falkow, S. (1989). Coordinate regulation and sensory transduction in the control of bacterial virulence. Science 243, 916-922.
31. Morris, J.Z., Tissenbaum, H.A., and Ruvkun, G.A. (1996). A phosphatidylinositol-3-OH kinase family member regulating longevity and diapause in Caenorhabditis elegans. Nature 382, 536-539.
32. Mukhopadhyay, P., Williams, J., and Mills, D. (1988). Molecular analysis of a pathogenicity locus in Pseudomonas syringae pv. syringae. J. Bacteriol. 170, 5479-5488.
33. Neidhardt, F.C., VanBogelen, R.A., and Vaughn, V. (1984). The genetics and regulation of heat-shock proteins. Annu. Rev. Genet. 18, 295-329.
34. Ohman, D.E., Goldberg, J.B., and Flynn, J.L. (1990). Molecular analysis of the genetic switch activating alginate production. In Pseudomonas: Biotransformations, Pathogenesis and Evolving Biotechnology, S. Silver, A.M. Chakrabarty, B.lglewski, and S. Kaplan, eds. (Washington, D.C.: American Society for Microbiology), pp. 28-35.
35. Poole, K. (1994). Bacterial multidrug resistance-emphasis on efflux mechanisms and Pseudomonas aeruginosa. J. Antimicrob. Chemother. 34, 453-456.
36. Poole, K., Heinrichs, D.E., and Neshat, S. (1993). Cloning and sequence analysis of an EnvCD homologue in Pseudomonas aeruginosa: regulation by iron and possible involvement in the secretion of the siderophore pyoverdine. Mol. Microbiol. 10, 529-544.
37. Rahme, LG., Stevens, E.J., Wolfort, S.F., Shao, J., Tompkins, R.G., and Ausubel, F.M. (1995). Common virulence factors for bacterial pathogenicity in plants and animals. Science 268, 1899-1902.
38. Rahme, LG., Tan, M.-W., Le, L, Wong, S.M., Tompkins, R.G., Calderwood, S.B., and Ausubel, F.M. (1997). Use of model plant hosts to identify Pseudomonas aeruginosa virulence factors. Proc. Natl. Acad. Sci. USA 94, 13245-13250.
39. Sage, A.E., Vasil, A.I., and Vasil, M.L. (1997). Molecular characterization of mutants affected in the osmoprotectant-dependent induction of phospholipase C in Pseudomonas aeruginosa PAO1. Mol. Microbiol. 23, 43-56.
40. Schinkel, A.H., Smit, J.J.M., van Tellingen, O., Beijnen, J.H., Wagenaar, E., van Deetmer, L, Mol, C.A.A.M., van der Valk, M.A., Robanus-Maandag, E.C., te Riele, H.P.J., et al. (1994). Disruption of the mouse mdrla P-glycoprotein gene leads to a deficiency in the blood-brain barrier and to increased sensitivity to drugs. Cell 77, 491-502.
41. Sorensen, R.U., and Joseph, F.J. (1993). Phenazine pigments in Pseudomonas aeruginosa infection. In Pseudomonas aeruginosa as an Opportunistic Pathogen, M. Campa, M. Bendenelli, and H. Friedman, eds. (New York: Plenum Press), pp. 43-57.
42. Strauss, E.J., and Falkow, S. (1997). Microbial pathogenesis: genomics and beyond. Science 276, 707-711.
43. Strom, M.S., and Lory, S. (1986). Cloning and expression of the pilin gene of Pseudomonas aeruginosa PAK in Escherichia coli. J. Bacteriol. 165, 367-372.
44. Sulston, J., and Hodgkin, J. (1988). Methods. In The Nematode Caenorhabditis elegans, W.B. Wood, ed. (Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press), pp. 587-606.
45. Tan, M.-W., Mahajan-Miklos, S., and Ausubel, F.M. (1999) Killing of Caenorhabditis elegans by Pseudomonas aeruginosa used to model mammalian bacterial pathogenesis. Proc. Natl. Acad. Sci. USA, in press.
46. Thiebaut, F., Tsuruo, T., Hamada, H., Gottesman, M.M., Pastan, I., and Willingham, M.C. (1987). Cellular localization of the multidrug resistance gene product in normal human tissues. Proc. Natl. Acad. Sei. USA. 84, 7735-7738.
47. Turner, J.M., and Messenger, A.J. (1986). Occurence, biochemistry and physiology of phenazine pigment production. Adv. Microb. Physiol. 27, 211-273.
48. Vanfleteren, J.R. (1993). Oxidative stress and aging in Caenorhabditis elegans. Biochem. J. 292, 605-608.
49. Wilson, R., Sykes, D.A., Watson, D., Rutman, A., Taylor, G.W., and Cole, P.J. (1988). Measurement of Pseudomonas aeruginosa phenazine pigments in sputum and assessment of their contribution to sputum sol toxicity for respiratory epithelium. Infect. Immun. 56, 2515-2517.
50. Wood, R.E. (1976). Pseudomonas the compromised host. Hosp. Pract. 11, 91-100.